Decreased Diversity of the Oral Microbiota of Patients with Hepatitis B Virus-Induced Chronic Liver Disease: A Pilot Project

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/12

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

26606973

DOI Digital object identifier for electronic documents.

10.1038/srep17098

URI

Authors

Ling Z, Liu X, Cheng Y, Jiang X, Jiang H, Wang Y, Li L

Journal

Scientific reports

Year

2015

Increasing evidence suggests that altered gut microbiota is implicated in the pathogenesis of hepatitis B virus-induced chronic liver disease (HBV-CLD). However, the structure and composition of the oral microbiota of patients with HBV-CLD remains unclear. High-throughput pyrosequencing showed that decreased oral bacterial diversity was found in patients with HBV-CLD. The Firmicutes/Bacteroidetes ratio was increased significantly, which indicated that dysbiosis of the oral microbiota participated in the process of HBV-CLD development. However, the changing patterns of the oral microbiota in patients with HBV-induced liver cirrhosis (LC) were almost similar to patients with chronic hepatitis B (CHB). HBV infection resulted in an increase in potential H2S- and CH3SH-producing phylotypes such as Fusobacterium, Filifactor, Eubacterium, Parvimonas and Treponema, which might contribute to the increased oral malodor. These key oral-derived phylotypes might invade into the gut as opportunistic pathogens and contribute to altering the composition of the gut microbiota. This study provided important clues that dysbiosis of the oral microbiota might be involved in the development of HBV-CLD. Greater understanding of the relationships between the dysbiosis of oral microbiota and the development of HBV-CLD might facilitate the development of non-invasive differential diagnostic procedures and targeted treatments of HBV-CLD patients harbouring specific oral phylotypes.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/12

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Dental plaque Bacterial plaque,Plaque,Dental plaque,dental plaque

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Chronic hepatitis B virus infection chronic Hepatitis B,chronic hepatitis B infection,chronic hepatitis B virus infection,hepatitis B infection, chronic,hepatitis B, chronic,Chronic hepatitis B virus infection

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: chronic hepatitis B

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: chronic hepatitis B

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 10

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V3

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Simpson Estimator of species richness and species evenness: more weight on species evenness: increased

Richness Number of species: decreased

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/12

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3, Text

Description: LEfSe was used to identify the most differentially abundant taxa in healthy control and CHB patient samples

Abundance in Group 1: increased abundance in chronic hepatitis B

NCBI	Quality Control	Links
Enterobacteriaceae
Enterobacterales
Yersinia
Lysobacterales
Filifactor
Lysobacteraceae
Peptostreptococcaceae
Eubacteriales
Clostridia

Revision editor(s): WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/12

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 3, Text

Description: LEfSe was used to identify the most differentially abundant taxa in healthy control and CHB patient samples

Abundance in Group 1: decreased abundance in chronic hepatitis B

NCBI	Quality Control	Links
Leptotrichiaceae
Leptotrichia
Flavobacteriales
Flavobacteriaceae
Flavobacteriia
Capnocytophaga

Revision editor(s): WikiWorks

Experiment 2

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Curated date: 2021/01/10

Curator: WikiWorks

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Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: liver cirrhosis

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: Not specified