Gut microbiota dysbiosis is associated with Henoch-Schönlein Purpura in children

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2023/01/1

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

29525681

DOI Digital object identifier for electronic documents.

10.1016/j.intimp.2018.03.003

URI

Authors

Wang X, Zhang L, Wang Y, Liu X, Zhang H, Liu Y, Shen N, Yang J, Gai Z

Journal

International immunopharmacology

Year

2018

Keywords:

Dysbiosis, Henoch-Schönlein Purpura, IgA, Microbiota

BACKGROUND: Alterations in the intestinal microbiota have been associated with the development of allergic diseases, such as asthma and food allergies. However, there is no report detailing the role of microbiota alterations in Henoch-Schönlein Purpura (HSP) development. METHOD: A total of 85 children with HSP and 70 healthy children were recruited for this study. Intestinal microbiota composition was analyzed by 16S rRNA gene-based pyrosequencing. Fecal microbial diversity and composition were compared. RESULT: We compared the gut microbiota of 155 subjects and found that children with HSP exhibited gut microbial dysbiosis. Lower microbial diversity and richness were found in HSP patients when compared to the control group. Based on an analysis of similarities, the composition of the microbiota in HSP patients was also different from that of the control group (r = 0.306, P = 0.001). The relative abundance of the bacterial genera Dialister (P < 0.0001), Roseburia (P < 0.0001), and Parasutterella (P < 0.0001) was significantly decreased in HSP children, while the relative abundance of Parabacteroides (P < 0.006) and Enterococcus (P < 0.0001) in these children was significantly increased. Based on Spearman correlation analysis, the LOS showed a significant negative (P < 0.05) correlation with the genera Paraprevotella and Roseburia. Meanwhile, IgA levels exhibited a significant negative (P < 0.01) correlation with the genus Bifidobacterium. CONCLUSIONS: Our results indicate that HSP is associated with significant compositional and structural changes in the gut microbiota. These results enhance the potential for future microbial-based therapies to improve the clinical outcome of HSP in children.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2023/01/1

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Henoch-Schoenlein purpura Allergic purpura,allergic purpura,Allergic purpura (disorder),Allergic purpura NOS (disorder),autoimmune purpura,Autoimmune purpura (disorder) [Ambiguous],Henoch-Schoenlein purpura,Henoch-Scholein purpura,Henoch-Schonlein Purpura,Henoch-Schonlein purpura,Henoch-Schonlein purpura (disorder),Henoch-Schönlein purpura,HSP,IgA vasculitis,Purpura, autoimmune,purpura, autoimmune,Purpura, Schoenlein-Henoch,Purpura: [allergic] or [Henoch-Schonlein allergy],Schoenlein-Henoch purpura,henoch-Schoenlein purpura

Group 0 name Corresponds to the control (unexposed) group for case-control studies: controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Henoch-Schönlein Purpura

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Henoch-Schönlein Purpura in children

Group 0 sample size Number of subjects in the control (unexposed) group: 70

Group 1 sample size Number of subjects in the case (exposed) group: 85

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V2

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 3

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Richness Number of species: decreased

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2023/01/1

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 6b

Description: The most differentially abundant taxa between Henoch-Schönlein Purpura (HSP) and controls

Abundance in Group 1: increased abundance in Henoch-Schönlein Purpura

NCBI	Quality Control	Links
Veillonella
Fusobacteriia
Fusobacteriota
Fusobacteriales
Enterococcus
Enterococcaceae
Veillonellaceae
Megamonas
Porphyromonadaceae
Parabacteroides

Revision editor(s): WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2023/01/1

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): Fatima, WikiWorks

Source: Figure 6b

Description: The most differentially abundant taxa between Henoch-Schönlein Purpura (HSP) and controls

Abundance in Group 1: decreased abundance in Henoch-Schönlein Purpura

NCBI	Quality Control	Links
Alcaligenaceae
Betaproteobacteria
Burkholderiales
Christensenellaceae
Clostridia
Dialister
Eubacteriales
Bacillota
Haemophilus
Klebsiella
Lachnospira
Lachnospira eligens
Lachnospiraceae
Parasutterella
Pasteurellaceae
Pasteurellales
Roseburia
Ruminiclostridium
Ruminococcus
Streptococcaceae
Streptococcus
Agathobacter rectalis

Revision editor(s): Fatima, WikiWorks