Postmenopause as a key factor in the composition of the Endometrial Cancer Microbiome (ECbiome)

Study information

Reviewed Marked as Reviewed by Atrayees on 2023-7-25

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

31844128

DOI Digital object identifier for electronic documents.

10.1038/s41598-019-55720-8

URI

Authors

Walsh DM, Hokenstad AN, Chen J, Sung J, Jenkins GD, Chia N, Nelson H, Mariani A, Walther-Antonio MRS

Journal

Scientific reports

Year

2019

Incidence rates for endometrial cancer (EC) are rising, particularly in postmenopausal and obese women. Previously, we showed that the uterine and vaginal microbiome distinguishes patients with EC from those without. Here, we sought to examine the impact of patient factors (such as menopause status, body mass index, and vaginal pH) in the microbiome in the absence of EC and how these might contribute to the microbiome signature in EC. We find that each factor independently alters the microbiome and identified postmenopausal status as the main driver of a polymicrobial network associated with EC (ECbiome). We identified Porphyromas somerae presence as the most predictive microbial marker of EC and we confirm this using targeted qPCR, which could be of use in detecting EC in high-risk, asymptomatic women. Given the established pathogenic behavior of P. somerae and accompanying network in tissue infections and ulcers, future investigation into their role in EC is warranted.

Experiment 1

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Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-29

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Lwaldron, WikiWorks, ChiomaBlessing

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Lower part of vagina Caudal vagina,Lower third of vagina,Lower vagina,Perineal part of vagina,Sinus vagina,Vagina lower part,Vaginal bulb,Lower part of vagina,lower part of vagina

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Postmenopausal Postmenopausal,postmenopausal

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Pre-menopausal patients

Group 1 name Corresponds to the case (exposed) group for case-control studies: Post-menopausal patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Post-menopausal patients without Endometrial cancer

Group 0 sample size Number of subjects in the control (unexposed) group: 49

Group 1 sample size Number of subjects in the case (exposed) group: 14

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 2 weeks

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V5

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Wald Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.1

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment: obesity, vaginal pH

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Richness Number of species: increased

Signature 2

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Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-29

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 2 + Table S1

Description: Bacterial OTUs differentially enriched among Post-menopausal patients without endometrial cancer compared to Pre-menopausal patients

Abundance in Group 1: increased abundance in Post-menopausal patients

NCBI	Quality Control	Links
Atopobium
Bacteroides
Blautia
Campylobacter
Clostridium
Dialister
Faecalibacterium
Gallicola
Luteococcus
Peptococcus
Peptoniphilus
Porphyromonas
Tepidimicrobium
Tessaracoccus
Varibaculum
Anaerococcus
unclassified Bacillota
Alloscardovia
Actinomyces
Moryella

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 2

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Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees, ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Endometrial cancer cancer of endometrium,endometrial Ca,endometrial cancer,endometrial neoplasm,endometrium cancer,malignant endometrial neoplasm,malignant endometrium neoplasm,malignant neoplasm of endometrium,neoplasm of endometrium,primary malignant neoplasm of endometrium,tumor of endometrium,Endometrial cancer

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Benign

Group 1 name Corresponds to the case (exposed) group for case-control studies: Endometrial cancer

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients undergoing hysterectomy for endometrial cancer

Group 0 sample size Number of subjects in the control (unexposed) group: 67

Group 1 sample size Number of subjects in the case (exposed) group: 57

Lab analysis

Statistical Analysis

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment: body mass index, menopause, vaginal pH

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Richness Number of species: unchanged

Signature 1

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Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4 + Table S2

Description: Bacterial OTUs differentially enriched among patients with endometrial cancer compared to Benign patients

Abundance in Group 1: decreased abundance in Endometrial cancer

NCBI	Quality Control	Links
Lactobacillus

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

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Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-29

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Figure 4 + Table S2

Description: Bacterial OTUs differentially enriched among patients with endometrial cancer compared to Benign patients

Abundance in Group 1: increased abundance in Endometrial cancer

NCBI	Quality Control	Links
Actinomyces
Arcanobacterium
Campylobacter
Finegoldia
Fusobacterium
Gallicola
Lactobacillus
Peptococcus
Peptoniphilus
Porphyromonas
Prevotella
Varibaculum
Anaerococcus

Revision editor(s): WikiWorks, ChiomaBlessing