Postmenopause as a key factor in the composition of the Endometrial Cancer Microbiome (ECbiome)

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Reviewed Marked as Reviewed by Atrayees on 2023-7-25
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Walsh DM, Hokenstad AN, Chen J, Sung J, Jenkins GD, Chia N, Nelson H, Mariani A, Walther-Antonio MRS
Journal
Scientific reports
Year
2019
Incidence rates for endometrial cancer (EC) are rising, particularly in postmenopausal and obese women. Previously, we showed that the uterine and vaginal microbiome distinguishes patients with EC from those without. Here, we sought to examine the impact of patient factors (such as menopause status, body mass index, and vaginal pH) in the microbiome in the absence of EC and how these might contribute to the microbiome signature in EC. We find that each factor independently alters the microbiome and identified postmenopausal status as the main driver of a polymicrobial network associated with EC (ECbiome). We identified Porphyromas somerae presence as the most predictive microbial marker of EC and we confirm this using targeted qPCR, which could be of use in detecting EC in high-risk, asymptomatic women. Given the established pathogenic behavior of P. somerae and accompanying network in tissue infections and ulcers, future investigation into their role in EC is warranted.

Experiment 1


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Lwaldron, WikiWorks

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Lower part of vagina Caudal vagina,Lower third of vagina,Lower vagina,Perineal part of vagina,Sinus vagina,Vagina lower part,Vaginal bulb,Lower part of vagina
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
endometrial cancer cancer of endometrium,endometrial Ca,endometrial cancer,endometrial neoplasm,endometrium cancer,malignant endometrial neoplasm,malignant endometrium neoplasm,malignant neoplasm of endometrium,neoplasm of endometrium,primary malignant neoplasm of endometrium,tumor of endometrium
Group 0 name Corresponds to the control (unexposed) group for case-control studies
pre
Group 1 name Corresponds to the case (exposed) group for case-control studies
post
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
patients undergoing hysterectomy
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
2 weeks

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V5
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
Linear Regression
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.1
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
vaginal pH, menopause, body mass index

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Figure 2, Table S1

Description: Bacterial OTUs differentially enriched among patients without EC by menopause status

Abundance in Group 1: increased abundance in post

NCBI Quality ControlLinks
candidate phylum NAG2

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Figure 2, Table S1

Description: Bacterial OTUs differentially enriched among patients without EC by menopause status

Abundance in Group 1: decreased abundance in post

NCBI Quality ControlLinks
Dialister
Peptoniphilus
Porphyromonas
Campylobacter
Actinomyces
Tessaracoccus
Atopobium
Peptococcus
Bacteroides
Tepidimicrobium
Clostridium
Luteococcus
Gallicola
Blautia
Faecalibacterium
Varibaculum

Revision editor(s): WikiWorks

Experiment 2


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
benign
Group 1 name Corresponds to the case (exposed) group for case-control studies
endometrial cancer
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
patients undergoing hysterectomy for endometrial cancer
Group 0 sample size Number of subjects in the control (unexposed) group
75
Group 1 sample size Number of subjects in the case (exposed) group
66

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Figure 4

Description: Bacterial OTUs differentially enriched among patients with and without EC

Abundance in Group 1: increased abundance in endometrial cancer

NCBI Quality ControlLinks
Anaerococcus
Lactobacillus

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Figure 4

Description: Bacterial OTUs differentially enriched among patients with and without EC

Abundance in Group 1: decreased abundance in endometrial cancer

NCBI Quality ControlLinks
Lactobacillus
Porphyromonas
Prevotella
Finegoldia
Peptoniphilus
Fusobacterium
Campylobacter
Actinomyces
Peptococcus
Gallicola
Varibaculum
Arcanobacterium

Revision editor(s): WikiWorks