Alteration in gut microbiota associated with hepatitis B and non-hepatitis virus related hepatocellular carcinoma

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/06/21

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

30675188

DOI Digital object identifier for electronic documents.

10.1186/s13099-018-0281-6

URI

Authors

Liu Q, Li F, Zhuang Y, Xu J, Wang J, Mao X, Zhang Y, Liu X

Journal

Gut pathogens

Year

2019

Keywords:

Dysbiosis, Gut microbiome, HBV, Hepatocellular carcinoma, Liver cancer

Background: The onset of hepatocellular carcinoma (HCC) ranked fifth malignancies all over the world. Increasing evidences showed that the distribution of HCC was related to the incidence of chronic hepatitis B virus (HBV) infection and other factors, such as alcoholism, aflatoxin B1 ingestion and obesity. Recent studies demonstrated that gut dysbiosis plays an important role in liver diseases. However, the researches on gut microbiota of HBV and non-HBV non-HCV related HCC have not been reported. In this study, we investigated the differences between the gut microbiota of HBV related HCC (B-HCC) and non-HBV non-HCV related HCC (NBNC-HCC), finally found some potential bacteria, linking different pathological mechanism of both types of HCCs. Results: We carried out 16S rRNA analyses in a cohort of 33 healthy controls, 35 individuals with HBV related HCC (B-HCC) and 22 individuals with non-HBV non-HCV (NBNC) related HCC (NBNC-HCC). We found that the species richness of fecal microbiota of B-HCC patients was much higher than other two groups. Interestingly, the feces of NBNC-HCC patients harbored more potential pro-inflammatory bacteria (Escherichia-Shigella, Enterococcus) and reduced levels of Faecalibacterium, Ruminococcus, Ruminoclostridium which results in decrease potential of anti-inflammatory short-chain fatty acids. The feces of NBNC-HCC patients had relatively fewer abundance of multiple biological pathways related to amino acid and glucose metabolism, but high level of transport and secretion in some types. However, the B-HCC patients had opposite results of bacterial composition and associated multiple biological pathways versus NBNC-HCC patients. Meanwhile, we found that aberrant network of gut microbiota occurred differently in B-HCC and NBNC-HCC patients. Conclusions: Our study indicated that B-HCC and NBNC-HCC patients showed differential abundance of bacteria involved in different functions or biological pathways. We suggested the modification of specific gut microbiota may provide the therapeutic benefit for B-HCC and NBNC-HCC.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/06/21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Peace Sandy

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Hepatocellular carcinoma adult hepatoma,adult primary hepatocellular carcinoma,cancer, hepatocellular,carcinoma of liver,carcinoma of liver cells,carcinoma of the liver cells,carcinoma, hepatocellular, malignant,HCC,hepatoblastoma,hepatoblastoma caused by somatic mutation,hepatocellular adenocarcinoma,hepatocellular cancer,hepatocellular carcinoma,hepatoma,liver and intrahepatic bile duct carcinoma,liver cancer,liver carcinoma,liver cell cancer (hepatocellular carcinoma),liver cell carcinoma,primary carcinoma of liver cells,primary carcinoma of the liver cells,Hepatocellular carcinoma

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: non-HBV non-HCV related HCC

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: HCC patients whose disease is not related to hepatitis B/hepatitis virus

Group 0 sample size Number of subjects in the control (unexposed) group: 33

Group 1 sample size Number of subjects in the case (exposed) group: 22

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: increased

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Curator: Fatima Zohra

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Source: Figure 3

Description: Differential microbial abundance between healthy controls and non-hepatitis virus related hepatocellular carcinoma patients

Abundance in Group 1: increased abundance in non-HBV non-HCV related HCC

NCBI	Quality Control	Links
Anaerotruncus

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Signature 2

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Source: Figure 3

Description: Differential microbial abundance comparing healthy controls to non-hepatitis virus related hepatocellular carcinoma patients

Abundance in Group 1: decreased abundance in non-HBV non-HCV related HCC

NCBI	Quality Control	Links
Eubacterium ventriosum
Lachnospira
Lachnospiraceae
Megamonas

Revision editor(s): Claregrieve1, WikiWorks

Experiment 2

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Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: HBV-related HCC patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: HBV-related HCC patients

Group 1 sample size Number of subjects in the case (exposed) group: 35

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/06/21

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure 3

Description: Differential microbial abundance comparing healthy controls to HBV-related hepatocellular carcinoma patients

Abundance in Group 1: increased abundance in HBV-related HCC patients

NCBI	Quality Control	Links
Anaerotruncus
Oscillospiraceae
Phascolarctobacterium
Prevotella

Revision editor(s): Claregrieve1, WikiWorks

Experiment 3

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Curated date: 2021/01/10

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Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: HBV related HCC patients

Group 1 name Corresponds to the case (exposed) group for case-control studies: non-HBV non-HCV patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: HCC patients whose disease is not related to hepatitis B/hepatitis virus