Shift in skin microbiota of Western European women across aging

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Jugé R, Rouaud-Tinguely P, Breugnot J, Servaes K, Grimaldi C, Roth MP, Coppin H, Closs B
Journal
Journal of applied microbiology
Year
2018
Keywords:
16S rRNA gene sequencing, Western European women, age-related changes, alpha diversity, beta diversity, microbiota, skin
AIMS: The objective of our study was to compare the microbiota diversity between two different age groups of Western European women. METHODS AND RESULTS: Skin-swab samples were collected directly on the forehead of 34 healthy Western European women: 17 younger (21-31 years old) and 17 older individuals (54-69 years old). Bacterial communities were evaluated using the 16S rRNA gene sequencing. Data revealed a higher alpha diversity on the skin of older individuals compared with younger ones. Overall microbiota structure was different between the two age groups, as demonstrated by beta diversity analysis, which also highlighted a high interpersonal variation within older individuals. Furthermore, taxonomic composition analysis showed both an increase in Proteobacteria and a decrease in Actinobacteria on the older skin. At the genus level, older skin exhibited a significant increase in Corynebacterium and a decrease in Propionibacterium relative abundance. CONCLUSIONS: Our study revealed a shift in the distribution of skin microbiota during chronological aging in Western European women. SIGNIFICANCE AND IMPACT OF STUDY: Altogether these results could become the basis to develop new approaches aiming to rebalance the skin microbiota, which is modified during the aging process.

Experiment 1


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects
France
Host species Species from which microbiome was sampled (if applicable)
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Skin of body Entire integument,Entire skin,Integument,Integumental organ,Pelt,Skin,Skin organ,Skin of body
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
age age
Group 0 name Corresponds to the control (unexposed) group for case-control studies
younger age
Group 1 name Corresponds to the case (exposed) group for case-control studies
older age European women
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
aging
Group 0 sample size Number of subjects in the control (unexposed) group
17
Group 1 sample size Number of subjects in the case (exposed) group
17
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
unchanged
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 4, Figure S1, Figure s2

Description: Taxonomic analysis of forehead microbiota using LEfSe between older and younger european women

Abundance in Group 1: increased abundance in older age European women

NCBI Quality ControlLinks
Arthrobacter
Sneathia
Moraxellaceae
Pseudomonadales
Corynebacterium
Halomonadaceae
Halomonas
Oceanospirillales
Neisseriaceae
Neisseriales
Betaproteobacteria
Pseudomonadota

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 4, Figure S1, Figure s2

Description: Taxonomic analysis of forehead microbiota using LEfSe between older and younger european women

Abundance in Group 1: decreased abundance in older age European women

NCBI Quality ControlLinks
Propionibacteriaceae
Propionibacterium
Propionibacteriales

Revision editor(s): WikiWorks