Microbe-host interplay in atopic dermatitis and psoriasis

From BugSigDB
Reviewed Marked as Reviewed by Folakunmi on 2024-2-26
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Fyhrquist N, Muirhead G, Prast-Nielsen S, Jeanmougin M, Olah P, Skoog T, Jules-Clement G, Feld M, Barrientos-Somarribas M, Sinkko H, van den Bogaard EH, Zeeuwen PLJM, Rikken G, Schalkwijk J, Niehues H, Däubener W, Eller SK, Alexander H, Pennino D, Suomela S, Tessas I, Lybeck E, Baran AM, Darban H, Gangwar RS, Gerstel U, Jahn K, Karisola P, Yan L, Hansmann B, Katayama S, Meller S, Bylesjö M, Hupé P, Levi-Schaffer F, Greco D, Ranki A, Schröder JM, Barker J, Kere J, Tsoka S, Lauerma A, Soumelis V, Nestle FO, Homey B, Andersson B, Alenius H
Journal
Nature communications
Year
2019
Despite recent advances in understanding microbial diversity in skin homeostasis, the relevance of microbial dysbiosis in inflammatory disease is poorly understood. Here we perform a comparative analysis of skin microbial communities coupled to global patterns of cutaneous gene expression in patients with atopic dermatitis or psoriasis. The skin microbiota is analysed by 16S amplicon or whole genome sequencing and the skin transcriptome by microarrays, followed by integration of the data layers. We find that atopic dermatitis and psoriasis can be classified by distinct microbes, which differ from healthy volunteers microbiome composition. Atopic dermatitis is dominated by a single microbe (Staphylococcus aureus), and associated with a disease relevant host transcriptomic signature enriched for skin barrier function, tryptophan metabolism and immune activation. In contrast, psoriasis is characterized by co-occurring communities of microbes with weak associations with disease related gene expression. Our work provides a basis for biomarker discovery and targeted therapies in skin dysbiosis.

Experiment 1


Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Folakunmi

Subjects

Location of subjects
Finland
Germany
United Kingdom
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Skin of body Entire integument,Entire skin,Integument,Integumental organ,Pelt,Skin,Skin organ,Skin of body,skin of body
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Atopic eczema allergic,allergic dermatitis,allergic form of dermatitis,Atopic Dermatitides,Atopic Dermatitis,Atopic dermatitis,atopic dermatitis and related conditions,Atopic dermatitis and related conditions (disorder),atopic eczema,Atopic Neurodermatitides,Atopic Neurodermatitis,Atopic neurodermatitis,Besnier's prurigo,Dermatitides, Atopic,Dermatitis, Atopic,Disseminated Neurodermatitides,Disseminated Neurodermatitis,eczema,Eczema, Atopic,Eczema, Infantile,eczematous dermatitis,Infantile Eczema,Neurodermatitides, Atopic,Neurodermatitides, Disseminated,Neurodermatitis, Atopic,Neurodermatitis, Disseminated,OTHER ATOPIC DERMATITIS,Other atopic dermatitis and related conditions,Atopic eczema
Group 0 name Corresponds to the control (unexposed) group for case-control studies
healthy control
Group 1 name Corresponds to the case (exposed) group for case-control studies
adult with atopic dermatitis
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
adult with moderate-to-severe chronic atopic dermatitis
Group 0 sample size Number of subjects in the control (unexposed) group
115
Group 1 sample size Number of subjects in the case (exposed) group
82
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
2 weeks

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks, Folakunmi

Source: Figure 1c

Description: Differentially abundant OTUs among adults with atopic dermatitis (AD) and healthy controls

Abundance in Group 1: increased abundance in adult with atopic dermatitis

NCBI Quality ControlLinks
Staphylococcus aureus

Revision editor(s): WikiWorks, Folakunmi

Signature 2

Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks, Folakunmi

Source: Figure 1c

Description: Differentially abundant OTUs among adults with atopic dermatitis (AD) and healthy controls

Abundance in Group 1: decreased abundance in adult with atopic dermatitis

NCBI Quality ControlLinks
Burkholderia sp.
Cutibacterium acnes
Finegoldia sp.
Lactobacillus sp.
Roseateles sp.
Staphylococcus sp.

Revision editor(s): WikiWorks, Folakunmi

Experiment 2


Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Folakunmi

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Psoriasis Other and unspecified pityriasis,OTHER PSORIASIS,Other psoriasis and similar disorders,Other psoriasis and similar disorders (disorder),Other psoriasis and similar disorders excluding psoriatic arthropathy,Palmoplantaris Pustulosis,PITYRIASIS NEC & NOS,PSORIAS RELATED DIS NEC,Psoriases,psoriasis,Psoriasis and similar disorders,Psoriasis and similar disorders (disorder),Psoriasis and similar disorders (navigational concept),Psoriasis and similar disorders NOS,Psoriasis and similar disorders NOS (disorder),Pustular Psoriasis of Palms and Soles,PUSTULAR PSORIASIS OF PALMS SOLES,Pustulosis of Palms and Soles,PUSTULOSIS OF PALMS SOLES,Pustulosis Palmaris et Plantaris,Psoriasis
Group 1 name Corresponds to the case (exposed) group for case-control studies
adult with psoriasis
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
adult with psoriasis
Group 1 sample size Number of subjects in the case (exposed) group
119

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks, Folakunmi

Source: Figure 1c

Description: Differentially abundant OTUs among adults with psoriasis (PSO) and healthy controls

Abundance in Group 1: increased abundance in adult with psoriasis

NCBI Quality ControlLinks
Anaerococcus sp.
Corynebacterium kroppenstedtii
Corynebacterium simulans
Finegoldia sp.
Neisseriaceae

Revision editor(s): WikiWorks, Folakunmi

Signature 2

Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks, Folakunmi

Source: Figure 1c

Description: Differentially abundant OTUs among adults with psoriasis (PSO) and healthy controls

Abundance in Group 1: decreased abundance in adult with psoriasis

NCBI Quality ControlLinks
Burkholderia sp.
Lactobacillus sp.
Roseateles sp.

Revision editor(s): WikiWorks, Folakunmi

Experiment 3


Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2024/02/26

Curator: Folakunmi

Revision editor(s): Folakunmi

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Atopic eczema allergic,allergic dermatitis,allergic form of dermatitis,Atopic Dermatitides,Atopic Dermatitis,Atopic dermatitis,atopic dermatitis and related conditions,Atopic dermatitis and related conditions (disorder),atopic eczema,Atopic Neurodermatitides,Atopic Neurodermatitis,Atopic neurodermatitis,Besnier's prurigo,Dermatitides, Atopic,Dermatitis, Atopic,Disseminated Neurodermatitides,Disseminated Neurodermatitis,eczema,Eczema, Atopic,Eczema, Infantile,eczematous dermatitis,Infantile Eczema,Neurodermatitides, Atopic,Neurodermatitides, Disseminated,Neurodermatitis, Atopic,Neurodermatitis, Disseminated,OTHER ATOPIC DERMATITIS,Other atopic dermatitis and related conditions,Atopic eczema
Group 1 name Corresponds to the case (exposed) group for case-control studies
adult with atopic dermatitis
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
adult with moderate-to-severe chronic atopic dermatitis
Group 1 sample size Number of subjects in the case (exposed) group
82

Lab analysis

Statistical Analysis

Statistical test
Mann-Whitney (Wilcoxon)
Kruskall-Wallis
Spearman Correlation
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.01
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
age, sex, Confounders controlled for: "anatomical location" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.anatomical location, Confounders controlled for: "clinic center" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.clinic center

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2024/02/26

Curator: Folakunmi

Revision editor(s): Folakunmi

Source: supplementary table 2

Description: Abundant OTUs that were significantly associated with disease and also significantly associated with a confounded effect.

Abundance in Group 1: increased abundance in adult with atopic dermatitis

NCBI Quality ControlLinks
Finegoldia sp.
Bradyrhizobium sp.
Anaerococcus sp.
Variovorax paradoxus
Lactobacillus sp.
Staphylococcus aureus

Revision editor(s): Folakunmi

Experiment 4


Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2024/02/26

Curator: Folakunmi

Revision editor(s): Folakunmi

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
adults with psoriasis
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
adult with moderate-to-severe chronic plaque-type psoriasis
Group 1 sample size Number of subjects in the case (exposed) group
119

Lab analysis

Statistical Analysis

Statistical test
Kruskall-Wallis
Mann-Whitney (Wilcoxon)
Spearman Correlation


Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-26

Curated date: 2024/02/26

Curator: Folakunmi

Revision editor(s): Folakunmi

Source: supplementary table 2

Description: Abundant OTUs that were significantly associated with disease and also significantly associated with a confounded effect.

Abundance in Group 1: increased abundance in adults with psoriasis

NCBI Quality ControlLinks
Finegoldia sp.
Bradyrhizobium sp.
Corynebacterium sp.
Corynebacterium kroppenstedtii

Revision editor(s): Folakunmi