Intestinal Microbiota Is Influenced by Gender and Body Mass Index/Experiment 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects
Spain
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Sex design sex_design,Sex design,sex design
Group 0 name Corresponds to the control (unexposed) group for case-control studies
male
Group 1 name Corresponds to the case (exposed) group for case-control studies
female
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
three groups according to the BMI: BMI < 30; BMI between 30- 33; and BMI > 33.
Group 0 sample size Number of subjects in the control (unexposed) group
39
Group 1 sample size Number of subjects in the case (exposed) group
36

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Matched on Factors on which subjects have been matched on in a case-control study
age


Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Marianthi Thomatos

Revision editor(s): WikiWorks

Source: Text + Table 2

Description: Intestinal Microbiota Is Influenced by Gender and Body Mass Index

Abundance in Group 1: increased abundance in female

NCBI Quality ControlLinks
Bilophila
Bacteroides caccae

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Marianthi Thomatos

Revision editor(s): WikiWorks

Source: Text + Table 2

Description: Intestinal Microbiota Is Influenced by Gender and Body Mass Index

Abundance in Group 1: decreased abundance in female

NCBI Quality ControlLinks
Phocaeicola plebeius

Revision editor(s): WikiWorks