Differences in Gut Microbiota Profiles between Autoimmune Pancreatitis and Chronic Pancreatitis

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1
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Hamada S, Masamune A, Nabeshima T, Shimosegawa T
The Tohoku journal of experimental medicine
Host-derived factors alter gut microenvironment, and changes in gut microbiota also affect biological functions of host. Alterations of gut microbiota have been reported in a wide variety of diseases, but the whole picture of alterations in pancreatic diseases remains to be clarified. In particular, the gut microbiota may be affected by malnutrition or impaired exocrine pancreas function that is associated with pancreatic diseases. We here conducted comprehensive analysis of gut microbiota in patients with type 1 autoimmune pancreatitis (AIP), a pancreatic manifestation of the systemic IgG4-related disease, and chronic pancreatitis (CP). The two diseases were selected, because altered immune reactions in AIP and/or long-standing malnutrition in CP may influence the gut microbiota. Fecal samples were obtained from 12 patients with AIP before the steroid therapy and 8 patients with CP. Metagenome DNA was extracted, and microbiota was analyzed by next generation sequencing. Gut microbiota profiles were different between patients with AIP and those with CP; namely, the proportions of Bacteroides, Streptococcus and Clostridium species were higher in patients with CP. The reasons for the increased proportion of these bacterial species remain unknown, but may reflect malabsorption and/or decreased pancreatic enzymes, both of which are associated with CP. Incidentally, the identified Streptococcus species are oral cavity inhabitants and also known as pathogens for endocarditis. Despite the small sample size, this study has shown the differences in gut microbiota profiles between AIP and CP. Comprehensive analysis of the gut microbiota may be useful for the differential diagnosis of pancreatic diseases.

Experiment 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks


Location of subjects
Host species Species from which microbiome was sampled (if applicable)
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
chronic pancreatitis chronic pancreatitis,Chronic pancreatitis (disorder),chronic pancreatitis (disorder) [Ambiguous],CP - Chronic pancreatitis,Pancreatitis, Chronic,pancreatitis, chronic,Pancreatitis, recurrent,Recurrent pancreatitis,Recurrent pancreatitis (disorder),Relapsing pancreatitis,Relapsing pancreatitis (disorder)
Group 0 name Corresponds to the control (unexposed) group for case-control studies
chronic pancreatitis
Group 1 name Corresponds to the case (exposed) group for case-control studies
autoimmune pancreatitis type 1
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
autoimmune pancreatitis type 1
Group 0 sample size Number of subjects in the control (unexposed) group
Group 1 sample size Number of subjects in the case (exposed) group

Lab analysis

Sequencing type
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Statistical Analysis

Statistical test
Fisher's Exact Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): Claregrieve1, Atrayees, WikiWorks

Source: Figure 2, text

Description: Differential microbial abundance between patients with type 1 autoimmune pancreatitis (AIP) and chronic pancreatitis (CP)

Abundance in Group 1: decreased abundance in autoimmune pancreatitis type 1

NCBI Links
Bacteroides ovatus
Clostridium lacusfryxellense
Enterocloster lavalensis
Streptococcus australis
Streptococcus gordonii
Anaerotignum lactatifermentans

Revision editor(s): Claregrieve1, Atrayees, WikiWorks