Differences in Gut Microbiota Profiles between Autoimmune Pancreatitis and Chronic Pancreatitis

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

29434076

DOI Digital object identifier for electronic documents.

10.1620/tjem.244.113

URI

Authors

Hamada S, Masamune A, Nabeshima T, Shimosegawa T

Journal

The Tohoku journal of experimental medicine

Year

2018

Keywords:

16S sequence, microbiome, next generation sequencing, pancreatic cancer, pancreatitis

Host-derived factors alter gut microenvironment, and changes in gut microbiota also affect biological functions of host. Alterations of gut microbiota have been reported in a wide variety of diseases, but the whole picture of alterations in pancreatic diseases remains to be clarified. In particular, the gut microbiota may be affected by malnutrition or impaired exocrine pancreas function that is associated with pancreatic diseases. We here conducted comprehensive analysis of gut microbiota in patients with type 1 autoimmune pancreatitis (AIP), a pancreatic manifestation of the systemic IgG4-related disease, and chronic pancreatitis (CP). The two diseases were selected, because altered immune reactions in AIP and/or long-standing malnutrition in CP may influence the gut microbiota. Fecal samples were obtained from 12 patients with AIP before the steroid therapy and 8 patients with CP. Metagenome DNA was extracted, and microbiota was analyzed by next generation sequencing. Gut microbiota profiles were different between patients with AIP and those with CP; namely, the proportions of Bacteroides, Streptococcus and Clostridium species were higher in patients with CP. The reasons for the increased proportion of these bacterial species remain unknown, but may reflect malabsorption and/or decreased pancreatic enzymes, both of which are associated with CP. Incidentally, the identified Streptococcus species are oral cavity inhabitants and also known as pathogens for endocarditis. Despite the small sample size, this study has shown the differences in gut microbiota profiles between AIP and CP. Comprehensive analysis of the gut microbiota may be useful for the differential diagnosis of pancreatic diseases.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Subjects

Location of subjects: Japan

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Chronic pancreatitis chronic pancreatitis,Chronic pancreatitis (disorder),chronic pancreatitis (disorder) [Ambiguous],CP - Chronic pancreatitis,Pancreatitis, Chronic,pancreatitis, chronic,Pancreatitis, recurrent,Recurrent pancreatitis,Recurrent pancreatitis (disorder),Relapsing pancreatitis,Relapsing pancreatitis (disorder),Chronic pancreatitis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: chronic pancreatitis

Group 1 name Corresponds to the case (exposed) group for case-control studies: autoimmune pancreatitis type 1

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: autoimmune pancreatitis type 1

Group 0 sample size Number of subjects in the control (unexposed) group: 8

Group 1 sample size Number of subjects in the case (exposed) group: 12

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4-V5

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: Fisher's Exact Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): Claregrieve1, Atrayees, WikiWorks

Source: Figure 2, text

Description: Differential microbial abundance between patients with type 1 autoimmune pancreatitis (AIP) and chronic pancreatitis (CP)

Abundance in Group 1: decreased abundance in autoimmune pancreatitis type 1

NCBI	Quality Control	Links
Bacteroides ovatus
Clostridium lacusfryxellense
Enterocloster lavalensis
Streptococcus australis
Streptococcus gordonii
Anaerotignum lactatifermentans

Revision editor(s): Claregrieve1, Atrayees, WikiWorks