Features of the bronchial bacterial microbiome associated with atopy, asthma, and responsiveness to inhaled corticosteroid treatment/Experiment 1
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Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13
Curated date: 2021/01/10
Curator: WikiWorks
Revision editor(s): Claregrieve1, WikiWorks, ChiomaBlessing
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Bronchus Bronchi,Bronchial tissue,Bronchial trunk,Bronchus,bronchus
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Atopic asthma allergic asthma,Atopic asthma,atopic asthma
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- atopic asthma
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients who have atopic asthma
- Group 0 sample size Number of subjects in the control (unexposed) group
- 21
- Group 1 sample size Number of subjects in the case (exposed) group
- 42
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- Negative Binomial Regression
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.1
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13
Source: Figure 2a, Supplemental Table S4
Description: Differentially abundant microbial taxa in atopic asthmatic (AA) subjects compared with healthy controls (HC)
Abundance in Group 1: increased abundance in atopic asthma
Revision editor(s): Claregrieve1, WikiWorks
Signature 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13
Source: Figure 2a, Supplemental Table S4
Description: Differential microbial abundance between atopic asthmatic (AA) subjects compared with healthy controls (HC)
Abundance in Group 1: decreased abundance in atopic asthma
| NCBI | Quality Control | Links |
|---|---|---|
| Actinobacillus | ||
| Kocuria palustris | ||
| Lacticaseibacillus zeae | ||
| Lactobacillus | ||
| Leptotrichia | ||
| Methylobacterium | ||
| Peptostreptococcus |
Revision editor(s): Claregrieve1, WikiWorks
