Features of the bronchial bacterial microbiome associated with atopy, asthma, and responsiveness to inhaled corticosteroid treatment/Experiment 4

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/14

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, ChiomaBlessing

Subjects

Location of subjects

United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Bronchus Bronchi,Bronchial tissue,Bronchial trunk,Bronchus,bronchus

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Atopic asthma allergic asthma,Atopic asthma,atopic asthma

Group 0 name Corresponds to the control (unexposed) group for case-control studies

nonresponder

Group 1 name Corresponds to the case (exposed) group for case-control studies

inhaled corticosteroid responder

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

inhaled corticosteroid (ICS) responder

Group 0 sample size Number of subjects in the control (unexposed) group

10

Group 1 sample size Number of subjects in the case (exposed) group

15

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

3 months

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

raw counts

Statistical test

Negative Binomial Regression

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.1

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/14

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): Claregrieve1, WikiWorks, Merit

Source: Figure 6b

Description: Differential relative taxon abundance in inhaled corticosteroid (ICS) responders versus nonresponders among those with atopic asthma

Abundance in Group 1: increased abundance in inhaled corticosteroid responder

NCBI	Quality Control	Links
Streptococcaceae
Erysipelotrichaceae
Fusobacteriaceae
Lachnospiraceae
Moraxellaceae
Neisseriaceae
Peptostreptococcaceae
Sphingomonadaceae
Veillonellaceae
unclassified Mogibacterium
Streptococcus

Revision editor(s): Claregrieve1, WikiWorks, Merit

Signature 2

EditHistoryDelete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/14

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure 6b

Description: Differential relative taxon abundance in inhaled corticosteroid (ICS) responders versus nonresponders among those with atopic asthma

Abundance in Group 1: decreased abundance in inhaled corticosteroid responder

NCBI	Quality Control	Links
Actinomycetaceae
Aerococcaceae
Azotobacter group
Flavobacteriaceae
Lactobacillaceae
Microbacteriaceae
Mycoplasmataceae
Pasteurellaceae
Porphyromonadaceae
Prevotellaceae
Spirochaetaceae
Weeksellaceae
Gemella

Revision editor(s): Claregrieve1, WikiWorks