Cervical microbiome is altered in cervical intraepithelial neoplasia after loop electrosurgical excision procedure in china

Study information

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

study design

time series / longitudinal observational

Citation

PMID PubMed identifier for scientific articles.

29563617

DOI Digital object identifier for electronic documents.

10.1038/s41598-018-23389-0

URI Uniform resource identifier for web resources.

Authors

Zhang H, Lu J, Lu Y, Cai Q, Liu H, Xu C

Journal

Scientific reports

Year

2018

Although human papillomavirus (HPV) infection is a major cause leading to the development of cervical intraepithelial neoplasia (CIN), the relationship between genital microbiome and HPV persistence/clearance is not well established. Loop electrosurgical excision procedure (LEEP) is one of standard treatments of CIN 2/3 globally, yet little is known about how the LEEP influence genital microbiota. We conducted a prospective study of 26 patients with CIN2/3 who underwent analysis of cervical microbiome before and after 3 months of LEEP treatment. Cervical swabs were collected, and microbiomes were analyzed by 16S ribosomal RNA gene sequencing. A decrease of cervical microbial diversity was observed after 3 months of LEEP treatment. Notably, a significant shift from community type of a Prevotella-containing and lack of a consistent dominant species to lactobacillus iners dominated microbiome correlated with LEEP. Particularly, Leptotrichia and clostridium were further decreased after LEEP treatment (P = 0.049 and P = 0.002, respectively). Our results suggest that the cervical microbiome is altered after LEEP treatment in patients with CIN2/3. Further studies with larger sample sizes are needed to validate these findings.

Experiment 1

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Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Uterine cervix Canalis cervicis uteri,Caudal segment of uterus,Cervical canal,Cervical canal of uterus,Cervix,Cervix of uterus,Cervix uteri,Neck of uterus,Uterine cervix

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: cervical glandular intraepithelial neoplasia cervical glandular intraepithelial neoplasia

Group 0 name Corresponds to the control (unexposed) group for case-control studies: after loop electrosurgical excision procedure
Group 1 name Corresponds to the case (exposed) group for case-control studies: before
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Cervical intraepithelial neoplasia 2/3 patients who underwent Loop electrosurgical excision procedure(LEEP)
Group 0 sample size Number of subjects in the control (unexposed) group: 26
Group 1 sample size Number of subjects in the case (exposed) group: 26
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 6 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa: unchanged
Richness Number of species: increased

Signature 1

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Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Atrayees, WikiWorks

Source: Figure 4

Description: Histogram of the LDA scores was used to features differentially abundant between LEEP and no LEEP state.

Abundance in Group 1: increased abundance in before

NCBI	Links
Actinomycetes
Bifidobacteriaceae
Bifidobacteriales
Burkholderiaceae
Clostridia
Clostridium
Collinsella
Corynebacteriaceae
Corynebacterium
Eubacteriales
Gardnerella vaginalis ⚠
Lachnospiraceae
Lactobacillus johnsonii
Leptotrichiaceae
Mycobacteriales
Oscillospiraceae
Peptostreptococcaceae
Prevotella amnii
Sneathia
Veillonella
Sneathia vaginalis