Temporal changes in gut microbiota profile in children with acute lymphoblastic leukemia prior to commencement-, during-, and post-cessation of chemotherapy/Experiment 1
Subjects
- Location of subjects
- Malaysia
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Leukemia blood (leukemia),leukemia,leukemia (disease),leukemia, disease,leukemia, malignant,leukemias,leukemias, general,Leukemia
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Acute Lymphoblastic Leukemia diagnosis and Pre- Chemotherapy treatment
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- children (ages 2-6 years old) diagnosed with and treated for Acute Lymphoblastic Leukemia
- Group 0 sample size Number of subjects in the control (unexposed) group
- 7
- Group 1 sample size Number of subjects in the case (exposed) group
- 7
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.1
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Matched on Factors on which subjects have been matched on in a case-control study
- age, ethnic group
Signature 1
Source: Figure 3a
Description: Differentially abundant bacteria were identified between Acute Lymphoblastic Leukemia (ALL) patients pre-chemotherapy and healthy controls
Abundance in Group 1: increased abundance in Acute Lymphoblastic Leukemia diagnosis and Pre- Chemotherapy treatment
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Bacteroides uniformis | ||
| Bacteroides fragilis |
Revision editor(s): WikiWorks
Signature 2
Source: Figure 3a
Description: Differentially abundant bacteria were identified between Acute Lymphoblastic Leukemia (ALL) patients pre-chemotherapy and healthy controls
Abundance in Group 1: decreased abundance in Acute Lymphoblastic Leukemia diagnosis and Pre- Chemotherapy treatment
| NCBI | Quality Control | Links |
|---|---|---|
| Campylobacter | ||
| Veillonellaceae | ||
| Corynebacterium | ||
| Bifidobacterium | ||
| Atopobium | ||
| Peptoniphilus | ||
| Staphylococcus | ||
| Prevotella | ||
| Anaerococcus | ||
| Porphyromonas |
Revision editor(s): WikiWorks
Signature 3
Source: Figure 3b
Description: Differentially abundant bacteria were identified between Acute Lymphoblastic Leukemia (ALL) patients post-chemotherapy and healthy controls
Abundance in Group 1: increased abundance in Acute Lymphoblastic Leukemia diagnosis and Pre- Chemotherapy treatment
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium |
Revision editor(s): WikiWorks
Signature 4
Source: Figure 3b
Description: Differentially abundant bacteria were identified between Acute Lymphoblastic Leukemia (ALL) patients post-chemotherapy and healthy controls
Abundance in Group 1: decreased abundance in Acute Lymphoblastic Leukemia diagnosis and Pre- Chemotherapy treatment
| NCBI | Quality Control | Links |
|---|---|---|
| Atopobium | ||
| Bacteroides | ||
| Prevotella | ||
| Fusobacterium | ||
| Corynebacterium |
Revision editor(s): WikiWorks
