Maternal omega-3 fatty acids regulate offspring obesity through persistent modulation of gut microbiota

From BugSigDB
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Robertson RC, Kaliannan K, Strain CR, Ross RP, Stanton C, Kang JX
Journal
Microbiome
Year
2018
Keywords:
Maternal diet, Microbiome, Microbiota, Obesity, n-3 PUFA
BACKGROUND: The early-life gut microbiota plays a critical role in host metabolism in later life. However, little is known about how the fatty acid profile of the maternal diet during gestation and lactation influences the development of the offspring gut microbiota and subsequent metabolic health outcomes. RESULTS: Here, using a unique transgenic model, we report that maternal endogenous n-3 polyunsaturated fatty acid (PUFA) production during gestation or lactation significantly reduces weight gain and markers of metabolic disruption in male murine offspring fed a high-fat diet. However, maternal fatty acid status appeared to have no significant effect on weight gain in female offspring. The metabolic phenotypes in male offspring appeared to be mediated by comprehensive restructuring of gut microbiota composition. Reduced maternal n-3 PUFA exposure led to significantly depleted Epsilonproteobacteria, Bacteroides, and Akkermansia and higher relative abundance of Clostridia. Interestingly, offspring metabolism and microbiota composition were more profoundly influenced by the maternal fatty acid profile during lactation than in utero. Furthermore, the maternal fatty acid profile appeared to have a long-lasting effect on offspring microbiota composition and function that persisted into adulthood after life-long high-fat diet feeding. CONCLUSIONS: Our data provide novel evidence that weight gain and metabolic dysfunction in adulthood is mediated by maternal fatty acid status through long-lasting restructuring of the gut microbiota. These results have important implications for understanding the interaction between modern Western diets, metabolic health, and the intestinal microbiome.

Experiment 1


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
Group 0 name Corresponds to the control (unexposed) group for case-control studies
foster mother fat1 pre HFD
Group 1 name Corresponds to the case (exposed) group for case-control studies
foster mother wild-type pre HFD
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
fat-1 mice: with a balanced tissue n-6/n-3 ratio (~ 1:1) and wild-type (WT) mice: with a high n-6/ n-3 ratio similar to the Western diet (> 10:1).
Group 0 sample size Number of subjects in the control (unexposed) group
15
Group 1 sample size Number of subjects in the case (exposed) group
9

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.4
Matched on Factors on which subjects have been matched on in a case-control study
age


Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Marianthi Thomatos

Revision editor(s): WikiWorks

Source: Text, Figure S4

Description: Pre-High fat diet (HFD) and Post-HF diet differential abundance in offspring according to foster mother genotype at lactation, wild type (WT) or fat-1 (balanced tissue n-6/n-3)

Abundance in Group 1: increased abundance in foster mother wild-type pre HFD

NCBI Quality ControlLinks
Clostridium
Clostridiaceae
Turicibacter
Erysipelotrichaceae
Peptococcaceae
Peptococcus
Flavonifractor
Erysipelotrichales
Erysipelotrichia
Butyricimonas
Oscillospiraceae
Butyricicoccus
Parabacteroides
Bacillota
Clostridia
Eubacteriales

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Marianthi Thomatos

Revision editor(s): WikiWorks

Source: Text, Figure S4

Description: Pre-High fat diet (HFD) and Post-HF diet differential abundance in offspring according to foster mother genotype at lactation, wild type (WT) or fat-1 (balanced tissue n-6/n-3)

Abundance in Group 1: decreased abundance in foster mother wild-type pre HFD

NCBI Quality ControlLinks
Bacteroidota
Bacteroidia
Bacteroidales
Campylobacterales
Campylobacterota
Pseudomonadota
Bacteroidaceae
Bacteroides
Deltaproteobacteria
Desulfovibrionales
Rikenellaceae
Alistipes
Barnesiella
Helicobacteraceae
Helicobacter
Desulfovibrionaceae
Odoribacter
Desulfovibrio
Allobaculum
Rikenella
Candidatus Saccharimonas

Revision editor(s): WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
foster mother fat1 post HFD
Group 1 name Corresponds to the case (exposed) group for case-control studies
foster mother wild-type post HFD

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
Not specified


Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Marianthi Thomatos

Revision editor(s): WikiWorks

Source: Text, Figure S4

Description: Pre-High fat diet (HFD) and Post-HF diet differential abundance in offspring according to foster mother genotype at lactation, wild type (WT) or fat-1 (balanced tissue n-6/n-3)

Abundance in Group 1: increased abundance in foster mother wild-type post HFD

NCBI Quality ControlLinks
Victivallis
Lentisphaera
Victivallaceae
Erysipelotrichaceae
Victivallales
Clostridium
Erysipelotrichales
Erysipelotrichia
Rikenella
Anaerotruncus
Oscillibacter
Flavonifractor
Lactobacillaceae
Lactobacillus
Peptococcus
Peptococcaceae
Lactobacillales
Bacilli
Butyricimonas
Oscillospiraceae
Lachnospiraceae
Parabacteroides
Porphyromonadaceae
Bacillota
Clostridia
Eubacteriales
Lentisphaeria

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Marianthi Thomatos

Revision editor(s): WikiWorks

Source: Text, Figure S4

Description: Pre-High fat diet (HFD) and Post-HF diet differential abundance in offspring according to foster mother genotype at lactation, wild type (WT) or fat-1 (balanced tissue n-6/n-3)

Abundance in Group 1: decreased abundance in foster mother wild-type post HFD

NCBI Quality ControlLinks
Campylobacterota
Campylobacterales
Pseudomonadota
Deltaproteobacteria
Desulfovibrionales
Helicobacteraceae
Helicobacter
Desulfovibrionaceae
Bacteroidaceae
Bacteroides
Odoribacter
Candidatus Saccharimonas

Revision editor(s): WikiWorks