Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-1-23

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

29362454

DOI Digital object identifier for electronic documents.

10.1038/s41598-018-19753-9

URI

Authors

Da Silva HE, Teterina A, Comelli EM, Taibi A, Arendt BM, Fischer SE, Lou W, Allard JP

Journal

Scientific reports

Year

2018

This study aimed to determine if there is an association between dysbiosis and nonalcoholic fatty liver disease (NAFLD) independent of obesity and insulin resistance (IR). This is a prospective cross-sectional study assessing the intestinal microbiome (IM) of 39 adults with biopsy-proven NAFLD (15 simple steatosis [SS]; 24 nonalcoholic steatohepatitis [NASH]) and 28 healthy controls (HC). IM composition (llumina MiSeq Platform) in NAFLD patients compared to HC were identified by two statistical methods (Metastats, Wilcoxon). Selected taxa was validated using quantitative PCR (qPCR). Metabolites in feces and serum were also analyzed. In NAFLD, 8 operational taxonomic units, 6 genera, 6 families and 2 phyla (Bacteroidetes, Firmicutes) were less abundant and; 1 genus (Lactobacillus) and 1 family (Lactobacillaceae) were more abundant compared to HC. Lower abundance in both NASH and SS patients compared to HC were confirmed by qPCR for Ruminococcus, Faecalibacterium prausnitzii and Coprococcus. No difference was found between NASH and SS. This lower abundance in NAFLD (NASH+SS) was independent of BMI and IR. NAFLD patients had higher concentrations of fecal propionate and isobutyric acid and serum 2-hydroxybutyrate and L-lactic acid. These findings suggest a potential role for a specific IM community and functional profile in the pathogenesis of NAFLD.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-23

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Peace Sandy

Subjects

Location of subjects: Canada

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Non-alcoholic fatty liver disease fatty liver disease, nonalcoholic,fatty liver disease, nonalcoholic, susceptibility to, 1,liver disease, alcoholic, susceptibility to, 1,NAFLD - Nonalcoholic Fatty Liver Disease,NAFLD - nonalcoholic fatty liver disease,NAFLD1,non-alcoholic fatty liver,non-alcoholic fatty liver disease,Nonalcoholic Fatty Liver Disease,nonalcoholic fatty liver disease,Non-alcoholic fatty liver disease

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy control

Group 1 name Corresponds to the case (exposed) group for case-control studies: NAFLD- [non alcoholic fatty liver disease]

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Adults with biopsy-proven steatosis and non-alcoholic fatty liver disease

Group 0 sample size Number of subjects in the control (unexposed) group: 28

Group 1 sample size Number of subjects in the case (exposed) group: 39

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 6 Months

Lab analysis

Sequencing type: PCR

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: Metastats

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-23

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks, Peace Sandy

Source: table 2

Description: Taxa identified as differentially abundant in patients with nonalocholic fatty liver disease (NAFLD) compared to HC

Abundance in Group 1: increased abundance in NAFLD- [non alcoholic fatty liver disease]

NCBI	Quality Control	Links
Lactobacillaceae
Lactobacillus
Bacilli

Revision editor(s): WikiWorks, Peace Sandy

Signature 2

Edit History Delete

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-23

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks, Peace Sandy

Source: Table 2

Description: Taxa identified as differentially abundant in patients with nonalocholic fatty liver disease (NAFLD) compared to HC

Abundance in Group 1: decreased abundance in NAFLD- [non alcoholic fatty liver disease]

NCBI	Quality Control	Links
Alistipes
Anaerostipes
Bacillota
Bacteroidaceae
Bacteroides
Bacteroidota
Blautia
Dorea
Faecalibacterium
Lachnospiraceae
Oscillospiraceae
Porphyromonadaceae
Rikenellaceae
Coprococcus
Roseburia
Parabacteroides
Lachnospiraceae incertae sedis
Clostridia
Bacteroidales

Revision editor(s): WikiWorks, Peace Sandy