Microbiota changes in a pediatric acute lymphocytic leukemia mouse model
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Curated date: 2021/01/10
Curator: WikiWorks743
Revision editor(s): WikiWorks753, WikiWorks743, Rimsha
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled (if applicable)
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Small intestine Anterior intestine,Intestinum tenue,Mid intestine,Small bowel,Small intestine
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- acute lymphoblastic leukemia acute lymphoblastic leukemia,acute lymphoblastic leukemia (ALL),acute lymphoblastic leukemia (disease),acute lymphoblastic leukemia/lymphoma,acute lymphocytic leukaemia,acute lymphocytic leukemia,acute lymphocytic leukemias,acute lymphogenous leukemia,acute lymphoid leukemia,ALL,ALL - acute lymphocytic leukemia,leukemia, lymphoblastic, malignant,lymphoblastic leukemia,lymphoblastic leukemia, acute,precursor cell lymphoblastic leukemia,precursor Lymphoblasic leukemia,precursor lymphoblastic leukemia
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Fecal bacterial composition in small intestines of control mice
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Fecal bacterial composition in small intestines of leukemia mice
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- three-week old female mice induced leukemia using lymphomblasts from pediatric acture lymphocytic leukemia patients from ATCC
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: Figure 4a, 4b and text
Description: Relative abundances and the ratio of relative abundances of the bacterial microbiota between the fecal compositions in the small intestine of control and leukemic mice model
Abundance in Group 1: increased abundance in Fecal bacterial composition in small intestines of leukemia mice
NCBI | Links |
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Oscillospira | |
Anaeroplasma | |
Butyrivibrio | |
Dorea | |
Ruminococcus | |
Dehalobacterium formicoaceticum | |
Clostridium | |
Coprococcus | |
Anaerostipes | |
Akkermansia | |
Blautia |
Revision editor(s): WikiWorks743
Signature 2
Source: Figure 4a, 4b and text
Description: Relative abundances and the ratio of relative abundances of the bacterial microbiota between the fecal compositions in the small intestine of control and leukemic mice model
Abundance in Group 1: decreased abundance in Fecal bacterial composition in small intestines of leukemia mice
NCBI | Links |
---|---|
Lactobacillus | |
Adlercreutzia | |
Turicibacter | |
Anaerostipes | |
Akkermansia | |
Blautia |
Revision editor(s): WikiWorks743