Microbiota changes in a pediatric acute lymphocytic leukemia mouse model

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-29

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

31884727

DOI Digital object identifier for electronic documents.

10.1002/mbo3.982

URI

Authors

Song Y, Gyarmati P

Journal

MicrobiologyOpen

Year

2020

Keywords:

high-throughput sequencing, leukemia, metagenomics, microbiota

Hematological malignancies are the most common type of pediatric cancers, and acute lymphocytic leukemia (ALL) is the most frequently occurring hematological malignancy during childhood. A major cause of mortality in leukemia is bloodstream infection (BSI). The aim of the current study was to explore the gut microbiota in ALL and its potential functional alterations. High-throughput sequencing was used to characterize the bacterial and fungal microbiota in feces and their predicted functional characteristics in a xenotransplant pediatric ALL mouse model. Our work shows that gut microbiota significantly changes in leukemia, which may result in functional alterations. This study may provide potential therapeutic or preventive strategies of BSI in ALL.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-29

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks, Peace Sandy

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Small intestine Anterior intestine,Intestinum tenue,Mid intestine,Small bowel,Small intestine,small intestine

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Acute lymphoblastic leukemia acute lymphoblastic leukemia,acute lymphoblastic leukemia (ALL),acute lymphoblastic leukemia (disease),acute lymphoblastic leukemia/lymphoma,acute lymphocytic leukaemia,acute lymphocytic leukemia,acute lymphocytic leukemias,acute lymphogenous leukemia,acute lymphoid leukemia,ALL,ALL - acute lymphocytic leukemia,leukemia, lymphoblastic, malignant,lymphoblastic leukemia,lymphoblastic leukemia, acute,precursor cell lymphoblastic leukemia,precursor Lymphoblasic leukemia,precursor lymphoblastic leukemia,Acute lymphoblastic leukemia

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy Control

Group 1 name Corresponds to the case (exposed) group for case-control studies: Fecal bacterial composition in small intestines of leukemia mice

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Three-week old female mice induced leukemia using lymphomblasts from pediatric acture lymphocytic leukemia patients from ATCC

Group 0 sample size Number of subjects in the control (unexposed) group: 8

Group 1 sample size Number of subjects in the case (exposed) group: 8

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: MGISEQ-2000

Statistical Analysis

Statistical test: T-Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-29

Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): WikiWorks, Peace Sandy

Source: Figure 4a, 4b and text

Description: Relative abundances (a) and the ratio of relative abundances (b) of the bacterial microbiota between the fecal compositions in the small intestine of control and leukemic mice

Abundance in Group 1: increased abundance in Fecal bacterial composition in small intestines of leukemia mice

NCBI	Quality Control	Links
Akkermansia
Anaeroplasma
Anaerostipes
Blautia
Butyrivibrio
Clostridium
Coprococcus
Dehalobacterium formicoaceticum
Dorea
Oscillospira
Ruminococcus

Revision editor(s): WikiWorks, Peace Sandy

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-29

Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): WikiWorks, Peace Sandy

Source: Figure 4a, 4b and text

Description: Relative abundances (a) and the ratio of relative abundances (b) of the bacterial microbiota between the fecal compositions in the small intestine of control and leukemic mice

Abundance in Group 1: decreased abundance in Fecal bacterial composition in small intestines of leukemia mice

NCBI	Quality Control	Links
Adlercreutzia
Lactobacillus
Turicibacter

Revision editor(s): WikiWorks, Peace Sandy

Experiment 2

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Curated date: 2024/03/07

Curator: Lwaldron

Revision editor(s): Lwaldron

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Group 1 name Corresponds to the case (exposed) group for case-control studies: Fecal bacterial composition of leukemia mice

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

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Curated date: 2024/03/07

Curator: Lwaldron

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Source: text (subset of Figure 2)

Description: Increased relative abundance in leukemic mice compared to control mice

Abundance in Group 1: increased abundance in Fecal bacterial composition of leukemia mice

NCBI	Quality Control	Links
Staphylococcus
Streptococcus
Ralstonia
Lactococcus

Revision editor(s): Lwaldron

Signature 2

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Curated date: 2024/03/07

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Source: Figure 2

Description: Decreased relative abundance in leukemic mice compared to control mice. I skipped the middle part of Figure 2 where the two groups look almost the same.

Abundance in Group 1: decreased abundance in Fecal bacterial composition of leukemia mice

NCBI	Quality Control	Links
Lactobacillus
Streptomyces
unclassified Clostridiaceae
Fusobacterium
Turicibacter
Akkermansia

Revision editor(s): Lwaldron