Microbiota changes in a pediatric acute lymphocytic leukemia mouse model

Study information

Needs review

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

31884727

DOI Digital object identifier for electronic documents.

10.1002/mbo3.982

URI

Authors

Song Y, Gyarmati P

Journal

MicrobiologyOpen

Year

2020

Keywords:

high-throughput sequencing, leukemia, metagenomics, microbiota

Hematological malignancies are the most common type of pediatric cancers, and acute lymphocytic leukemia (ALL) is the most frequently occurring hematological malignancy during childhood. A major cause of mortality in leukemia is bloodstream infection (BSI). The aim of the current study was to explore the gut microbiota in ALL and its potential functional alterations. High-throughput sequencing was used to characterize the bacterial and fungal microbiota in feces and their predicted functional characteristics in a xenotransplant pediatric ALL mouse model. Our work shows that gut microbiota significantly changes in leukemia, which may result in functional alterations. This study may provide potential therapeutic or preventive strategies of BSI in ALL.

Experiment 1

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Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled (if applicable): Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Small intestine Anterior intestine,Intestinum tenue,Mid intestine,Small bowel,Small intestine

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: acute lymphoblastic leukemia acute lymphoblastic leukemia,acute lymphoblastic leukemia (ALL),acute lymphoblastic leukemia (disease),acute lymphoblastic leukemia/lymphoma,acute lymphocytic leukaemia,acute lymphocytic leukemia,acute lymphocytic leukemias,acute lymphogenous leukemia,acute lymphoid leukemia,ALL,ALL - acute lymphocytic leukemia,leukemia, lymphoblastic, malignant,lymphoblastic leukemia,lymphoblastic leukemia, acute,precursor cell lymphoblastic leukemia,precursor Lymphoblasic leukemia,precursor lymphoblastic leukemia

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Fecal bacterial composition in small intestines of control mice
Group 1 name Corresponds to the case (exposed) group for case-control studies: Fecal bacterial composition in small intestines of leukemia mice
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: three-week old female mice induced leukemia using lymphomblasts from pediatric acture lymphocytic leukemia patients from ATCC
Group 0 sample size Number of subjects in the control (unexposed) group: 8
Group 1 sample size Number of subjects in the case (exposed) group: 8

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: T-Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

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Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): WikiWorks

Source: Figure 4a, 4b and text

Description: Relative abundances and the ratio of relative abundances of the bacterial microbiota between the fecal compositions in the small intestine of control and leukemic mice model

Abundance in Group 1: increased abundance in Fecal bacterial composition in small intestines of leukemia mice

NCBI	Quality Control	Links
Oscillospira
Anaeroplasma
Butyrivibrio
Dorea
Ruminococcus
Dehalobacterium formicoaceticum
Clostridium
Coprococcus
Anaerostipes
Akkermansia
Blautia

Revision editor(s): WikiWorks

Signature 2

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): WikiWorks

Source: Figure 4a, 4b and text

Description: Relative abundances and the ratio of relative abundances of the bacterial microbiota between the fecal compositions in the small intestine of control and leukemic mice model

Abundance in Group 1: decreased abundance in Fecal bacterial composition in small intestines of leukemia mice

NCBI	Quality Control	Links
Lactobacillus
Adlercreutzia
Turicibacter
Anaerostipes
Akkermansia
Blautia

Revision editor(s): WikiWorks