Gut microbiota in early pediatric multiple sclerosis: a case-control study/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Atrayees on 2023-6-30
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Multiple sclerosis Disseminated Sclerosis,generalized multiple sclerosis,insular sclerosis,MS,MS (Multiple Sclerosis),multiple sclerosis,MULTIPLE SCLEROSIS ACUTE FULMINATING,Multiple Sclerosis, Acute Fulminating,Sclerosis, Disseminated,Sclerosis, Multiple,Multiple sclerosis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- pediatric multiple sclerosis
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- early pediatric multiple sclerosis
- Group 0 sample size Number of subjects in the control (unexposed) group
- 17
- Group 1 sample size Number of subjects in the case (exposed) group
- 18
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 2 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- Negative Binomial Regression
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, ethnic group, breast feeding, sex
Alpha Diversity
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-30
Source: Figure 3a, 3b
Description: Difference between multiple sclerosis and controls in the gut microbial communities
Abundance in Group 1: increased abundance in pediatric multiple sclerosis
| NCBI | Quality Control | Links |
|---|---|---|
| Bilophila | ||
| Bifidobacterium | ||
| Eubacteriales | ||
| Desulfovibrio | ||
| Coriobacteriaceae | ||
| Segatella copri | ||
| Catenibacterium | ||
| Christensenellaceae | ||
| Oscillospiraceae | ||
| Phascolarctobacterium |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-30
Source: Figure 3a, 3b
Description: Difference between multiple sclerosis and controls in the gut microbial communities
Abundance in Group 1: decreased abundance in pediatric multiple sclerosis
| NCBI | Quality Control | Links |
|---|---|---|
| Lachnospira | ||
| Oscillospiraceae | ||
| Paraprevotella | ||
| Eubacteriales | ||
| Segatella copri | ||
| Bacteroides | ||
| Oscillospira | ||
| Peptostreptococcus anaerobius |
Revision editor(s): WikiWorks
