Altered fecal microbiota composition in patients with major depressive disorder
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Jiang H, Ling Z, Zhang Y, Mao H, Ma Z, Yin Y, Wang W, Tang W, Tan Z, Shi J, Li L, Ruan B
Journal
Brain, behavior, and immunity
Year
2015
Keywords:
Antidepressant, Depression, Gut bacteria, Gut–brain, Inflammation
Studies using animal models have shown that depression affects the stability of the microbiota, but the actual structure and composition in patients with major depressive disorder (MDD) are not well understood. Here, we analyzed fecal samples from 46 patients with depression (29 active-MDD and 17 responded-MDD) and 30 healthy controls (HCs). High-throughput pyrosequencing showed that, according to the Shannon index, increased fecal bacterial α-diversity was found in the active-MDD (A-MDD) vs. the HC group but not in the responded-MDD (R-MDD) vs. the HC group. Bacteroidetes, Proteobacteria, and Actinobacteria strongly increased in level, whereas that of Firmicutes was significantly reduced in the A-MDD and R-MDD groups compared with the HC group. Despite profound interindividual variability, levels of several predominant genera were significantly different between the MDD and HC groups. Most notably, the MDD groups had increased levels of Enterobacteriaceae and Alistipes but reduced levels of Faecalibacterium. A negative correlation was observed between Faecalibacterium and the severity of depressive symptoms. These findings enable a better understanding of changes in the fecal microbiota composition in such patients, showing either a predominance of some potentially harmful bacterial groups or a reduction in beneficial bacterial genera. Further studies are warranted to elucidate the temporal and causal relationships between gut microbiota and depression and to evaluate the suitability of the microbiome as a biomarker.
Experiment 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Major depressive disorder
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy Controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Active Major Depressive Disorder
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The A-MDD group was defined as having an HAMDS score P20. The patients in the R-MDD group were defined as those with a baseline HAMDS scores P20 upon admission to the hospi- tal.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 30
- Group 1 sample size Number of subjects in the case (exposed) group
- 29
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V3
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Roche454
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Needs review
Source: Figure 2
Description: Taxonomic differences of fecal microbiota between Healthy Controls and Active Major Depressive Disorder groups
Abundance in Group 1: increased abundance in Active Major Depressive Disorder
| NCBI | Quality Control | Links |
|---|---|---|
| Flavonifractor | ||
| Butyricimonas | ||
| Enterobacterales | ||
| Enterobacteriaceae | ||
| Parabacteroides | ||
| Porphyromonadaceae | ||
| Alistipes | ||
| Rikenellaceae |
Revision editor(s): WikiWorks
Signature 2
Needs review
Source: Figure 2
Description: Taxonomic differences of fecal microbiota between Healthy Controls and Active Major Depressive Disorder groups
Abundance in Group 1: decreased abundance in Active Major Depressive Disorder
| NCBI | Quality Control | Links |
|---|---|---|
| Oscillospiraceae | ||
| Faecalibacterium | ||
| Dialister | ||
| Pasteurellaceae | ||
| Pasteurellales | ||
| Ruminococcus | ||
| Haemophilus | ||
| Peptostreptococcaceae | ||
| Escherichia | ||
| Shigella |
Revision editor(s): WikiWorks
Experiment 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Responded Major Depressive Disorder
- Group 1 sample size Number of subjects in the case (exposed) group
- 17
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- Not specified
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Needs review
Source: Figure 3
Description: Taxonomic differences of fecal microbiota between Healthy Controls and Responded Major Depressive Disorder groups
Abundance in Group 1: increased abundance in Responded Major Depressive Disorder
| NCBI | Quality Control | Links |
|---|---|---|
| Butyricimonas | ||
| Porphyromonadaceae |
Revision editor(s): WikiWorks
Signature 2
Needs review
Source: Figure 3
Description: Taxonomic differences of fecal microbiota between Healthy Controls and Responded Major Depressive Disorder groups
Abundance in Group 1: decreased abundance in Responded Major Depressive Disorder
| NCBI | Quality Control | Links |
|---|---|---|
| Ruminococcus | ||
| Pasteurellaceae | ||
| Pasteurellales | ||
| Haemophilus | ||
| Clostridium | ||
| Clostridiaceae |
Revision editor(s): WikiWorks
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