Disrupted tongue microbiota and detection of nonindigenous bacteria on the day of allogeneic hematopoietic stem cell transplantation

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

32150591

DOI Digital object identifier for electronic documents.

10.1371/journal.ppat.1008348

URI

Authors

Oku S, Takeshita T, Futatsuki T, Kageyama S, Asakawa M, Mori Y, Miyamoto T, Hata J, Ninomiya T, Kashiwazaki H, Yamashita Y

Journal

PLoS pathogens

Year

2020

Disruption of the intestinal microbiota caused by intensive chemotherapy, irradiation and antibiotics can result in development of severe gut graft-versus-host disease and infectious complications, leading to poorer outcomes among allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. Although the oral cavity is also densely colonized by indigenous microorganisms, the bacterial composition in allo-HSCT recipients remains unclear. We determined the tongue microbiota composition of 45 patients with hematological disorders on the day of transplantation and compared them to 164 community-dwelling adults. The V1-V2 regions of the 16S rRNA gene sequences demonstrated that the allo-HSCT recipients had less diverse and distinct microbiota from that of community-dwelling adults. The full-length 16S rRNA gene sequences identified 146 bacterial taxa in the microbiota of allo-HSCT recipients, of which 34 bacterial taxa did not correspond to bacteria primarily inhabiting the oral cavity deposited in the expanded Human Oral Microbiome Database. Notably, the detection of Staphylococcus haemolyticus and/or Ralstonia pickettii was significantly associated with a higher risk of mortality during the follow-up period. These results demonstrate that the oral cavity of allo-HSCT recipients is colonized by a disrupted microbiota on the day of transplantation and suggest that detection of specific nonindigenous taxa could be a predictor of transplant outcome.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Subjects

Location of subjects: Japan

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Tongue Glossus,Tongue,tongue

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Response to allogeneic hematopoietic stem cell transplant Response to allogeneic hematopoietic stem cell transplant,response to allogeneic hematopoietic stem cell transplant

Group 0 name Corresponds to the control (unexposed) group for case-control studies: community-dwelling controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: allogeneic-hematopoietic stem cell transplant

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: patient ages 36-69 years old undergoing allogeneic- hematopoietic stem cell transplantation

Group 0 sample size Number of subjects in the control (unexposed) group: 164

Group 1 sample size Number of subjects in the case (exposed) group: 45

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V2

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Ion Torrent

Statistical Analysis

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Matched on Factors on which subjects have been matched on in a case-control study: age

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Richness Number of species: decreased

Signature 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): Claregrieve1, WikiWorks

Source: Fig. 2

Description: Differential abundances of predominant bacterial genera in the tongue microbiota of community-dwelling adults and allogeneic-hematopoietic stem cell transplantation patients

Abundance in Group 1: decreased abundance in allogeneic-hematopoietic stem cell transplant

NCBI	Quality Control	Links
Alloprevotella
Haemophilus
Leptotrichia
Neisseria
Oribacterium
Prevotella
Streptococcus

Revision editor(s): Claregrieve1, WikiWorks