Gut microbiota is critical for the induction of chemotherapy-induced pain

Study information

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

28714953

DOI Digital object identifier for electronic documents.

10.1038/nn.4606

URI

Authors

Shen S, Lim G, You Z, Ding W, Huang P, Ran C, Doheny J, Caravan P, Tate S, Hu K, Kim H, McCabe M, Huang B, Xie Z, Kwon D, Chen L, Mao J

Journal

Nature neuroscience

Year

2017

Chemotherapy-induced pain is a dose-limiting condition that affects 30% of patients undergoing chemotherapy. We found that gut microbiota promotes the development of chemotherapy-induced mechanical hyperalgesia. Oxaliplatin-induced mechanical hyperalgesia was reduced in germ-free mice and in mice pretreated with antibiotics. Restoring the microbiota of germ-free mice abrogated this protection. These effects appear to be mediated, in part, by TLR4 expressed on hematopoietic cells, including macrophages.

Experiment 1

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Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22

Curated date: 2024/02/22

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Response to antibiotic Response to antibiotic,response to antibiotic

Group 0 name Corresponds to the control (unexposed) group for case-control studies: H2O mice

Group 1 name Corresponds to the case (exposed) group for case-control studies: Exposed abx mice

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Mice fed with antibiotic water

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 10

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): None

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: RT-qPCR

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: ANOVA

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Signature 1

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Curated date: 2024/02/22

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Figure 1C

Description: Phylum analysis of altered microbiota in mice after antibiotic water feeding compared to mice fed with regular water

Abundance in Group 1: increased abundance in Exposed abx mice

NCBI	Quality Control	Links
Cyanobacteriota
Pseudomonadota
Bacillota

Revision editor(s): ChiomaBlessing

Signature 2

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Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22

Curated date: 2024/02/22

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing, Scholastica, Joan Chuks

Source: Figure 1C

Description: Phylum analysis of altered microbiota in mice after antibiotic water feeding compared to mice fed with regular water

Abundance in Group 1: decreased abundance in Exposed abx mice

NCBI	Quality Control	Links
Actinomycetota
Bacteroidota
Fusobacteriia
Mycoplasmatota
Verrucomicrobiia
Candidatus Saccharibacteria

Revision editor(s): ChiomaBlessing, Scholastica, Joan Chuks