Gut microbiota is critical for the induction of chemotherapy-induced pain

From BugSigDB
Needs review
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Shen S, Lim G, You Z, Ding W, Huang P, Ran C, Doheny J, Caravan P, Tate S, Hu K, Kim H, McCabe M, Huang B, Xie Z, Kwon D, Chen L, Mao J
Journal
Nature neuroscience
Year
2017
Chemotherapy-induced pain is a dose-limiting condition that affects 30% of patients undergoing chemotherapy. We found that gut microbiota promotes the development of chemotherapy-induced mechanical hyperalgesia. Oxaliplatin-induced mechanical hyperalgesia was reduced in germ-free mice and in mice pretreated with antibiotics. Restoring the microbiota of germ-free mice abrogated this protection. These effects appear to be mediated, in part, by TLR4 expressed on hematopoietic cells, including macrophages.

Experiment 1


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
graft versus host disease disease, graft-versus-host,disease, graft-VS-host,disease, homologous wasting,disease, runt,diseases, graft-versus-host,diseases, graft-VS-host,graft versus host disease,graft VS host disease,graft VS. host disease,graft-versus-host disease,graft-versus-host disease, resistance to,graft-versus-host disease, susceptibility to,graft-versus-host diseases,graft-versus-host-disease,graft-VS-host disease,graft-VS-host diseases,GVH,GVHD,GVHDS,homologous wasting disease,runt disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
non- graft- versus- host disease
Group 1 name Corresponds to the case (exposed) group for case-control studies
acute graft- versus- host disease
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
individual's receiving Hematopoietic cell transplantation (HCT) and having a grade 1-4 for acute graft-versus-host-disease
Group 0 sample size Number of subjects in the control (unexposed) group
14
Group 1 sample size Number of subjects in the case (exposed) group
52

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
decreased

Signature 1

Needs review

Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): WikiWorks

Source: Figure 2, text, table 2

Description: Specific Bacteria at neutrophil recovery (more than 500 neutrophils per millimeter cube) correlated with subsequent development of Severe Acute Graft-versus-Host Disease in patient's receiving Hematopoietic cell transplantation (HCT)

Abundance in Group 1: increased abundance in acute graft- versus- host disease

NCBI Quality ControlLinks
Roseburia hominis
Eisenbergiella sp.
Veillonella parvula
Enterococcus faecium
Enterococcus hirae
Ligilactobacillus salivarius
Limosilactobacillus fermentum
Phocaeicola dorei
Solobacterium moorei
Rothia mucilaginosa

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): WikiWorks, Merit

Source: Figure 2, text, table 2

Description: Specific Bacteria at neutrophil recovery (more than 500 neutrophils per millimeter cube) correlated with subsequent development of Severe Acute Graft-versus-Host Disease in patient's receiving Hematopoietic cell transplantation (HCT)

Abundance in Group 1: decreased abundance in acute graft- versus- host disease

NCBI Quality ControlLinks
Bacteroides caccae
Bacteroides ovatus
Bacteroides thetaiotaomicron
Blautia luti
Blautia sp.
Butyricicoccus sp.
Candidatus Soleaferrea
Dorea sp.
Ruminococcus sp.

Revision editor(s): WikiWorks, Merit