Gut microbiota is critical for the induction of chemotherapy-induced pain

Study information

Needs review

study design

time series / longitudinal observational

Citation

PMID PubMed identifier for scientific articles.

28714953

DOI Digital object identifier for electronic documents.

10.1038/nn.4606

URI Uniform resource identifier for web resources.

Authors

Shen S, Lim G, You Z, Ding W, Huang P, Ran C, Doheny J, Caravan P, Tate S, Hu K, Kim H, McCabe M, Huang B, Xie Z, Kwon D, Chen L, Mao J

Journal

Nature neuroscience

Year

2017

Chemotherapy-induced pain is a dose-limiting condition that affects 30% of patients undergoing chemotherapy. We found that gut microbiota promotes the development of chemotherapy-induced mechanical hyperalgesia. Oxaliplatin-induced mechanical hyperalgesia was reduced in germ-free mice and in mice pretreated with antibiotics. Restoring the microbiota of germ-free mice abrogated this protection. These effects appear to be mediated, in part, by TLR4 expressed on hematopoietic cells, including macrophages.

Experiment 1

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Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: graft versus host disease disease, graft-versus-host,disease, graft-VS-host,disease, homologous wasting,disease, runt,diseases, graft-versus-host,diseases, graft-VS-host,graft versus host disease,graft VS host disease,graft VS. host disease,graft-versus-host disease,graft-versus-host disease, resistance to,graft-versus-host disease, susceptibility to,graft-versus-host diseases,graft-versus-host-disease,graft-VS-host disease,graft-VS-host diseases,GVH,GVHD,GVHDS,homologous wasting disease,runt disease

Group 0 name Corresponds to the control (unexposed) group for case-control studies: non- graft- versus- host disease
Group 1 name Corresponds to the case (exposed) group for case-control studies: acute graft- versus- host disease
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: individual's receiving Hematopoietic cell transplantation (HCT) and having a grade 1-4 for acute graft-versus-host-disease
Group 0 sample size Number of subjects in the control (unexposed) group: 14
Group 1 sample size Number of subjects in the case (exposed) group: 52

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: T-Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa: decreased

Signature 1

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Curated date: 2021/01/10

Curator: William Lam

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Source: Figure 2, text, table 2

Description: Specific Bacteria at neutrophil recovery (more than 500 neutrophils per millimeter cube) correlated with subsequent development of Severe Acute Graft-versus-Host Disease in patient's receiving Hematopoietic cell transplantation (HCT)

Abundance in Group 1: increased abundance in acute graft- versus- host disease

NCBI	Links
Roseburia hominis
Eisenbergiella sp.
Veillonella parvula
Enterococcus faecium
Enterococcus hirae
Ligilactobacillus salivarius
Limosilactobacillus fermentum
Phocaeicola dorei
Solobacterium moorei
Rothia mucilaginosa

Revision editor(s): WikiWorks

Signature 2

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Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: William Lam

Revision editor(s): WikiWorks, Merit

Source: Figure 2, text, table 2

Description: Specific Bacteria at neutrophil recovery (more than 500 neutrophils per millimeter cube) correlated with subsequent development of Severe Acute Graft-versus-Host Disease in patient's receiving Hematopoietic cell transplantation (HCT)

Abundance in Group 1: decreased abundance in acute graft- versus- host disease

NCBI	Links
Bacteroides caccae
Bacteroides ovatus
Bacteroides thetaiotaomicron
Blautia luti
Blautia sp.
Butyricicoccus sp.
Candidatus Soleaferrea
Dorea sp.
Ruminococcus sp.

Revision editor(s): WikiWorks, Merit