The vaginal microbiota associates with the regression of untreated cervical intraepithelial neoplasia 2 lesions/Experiment 2

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Reviewed Marked as Reviewed by Fatima on 2021/07/16

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks, Victoria

Subjects

Location of subjects

United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Uterine cervix Canalis cervicis uteri,Caudal segment of uterus,Cervical canal,Cervical canal of uterus,Cervix,Cervix of uterus,Cervix uteri,Neck of uterus,Uterine cervix,uterine cervix

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Cervical glandular intraepithelial neoplasia Cervical glandular intraepithelial neoplasia,cervical glandular intraepithelial neoplasia

Group 0 name Corresponds to the control (unexposed) group for case-control studies

CIN regression

Group 1 name Corresponds to the case (exposed) group for case-control studies

CIN non-regression

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

histology proven CIN2, no regression after 12 months

Group 0 sample size Number of subjects in the control (unexposed) group

42

Group 1 sample size Number of subjects in the case (exposed) group

45

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V1-V2

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

raw counts

Statistical test

Welch's T-Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Alpha Diversity

Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa

unchanged

Richness Number of species

increased

Signature 1

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Your review change was submitted

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks

Source: Figure 4

Description: Outcomes at 12-month follow-up according to baseline VMB composition at baseline

Abundance in Group 1: increased abundance in CIN non-regression

NCBI	Quality Control	Links
Hoylesella timonensis
Gardnerella vaginalis

Revision editor(s): WikiWorks