Intestinal microbiota development in preterm neonates and effect of perinatal antibiotics

From BugSigDB
Needs review Marked as Needs review by Chloe on 2023-11-16
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Arboleya S, Sánchez B, Milani C, Duranti S, Solís G, Fernández N, de los Reyes-Gavilán CG, Ventura M, Margolles A, Gueimonde M
Journal
The Journal of pediatrics
Year
2015
OBJECTIVES: To assess the establishment of the intestinal microbiota in very low birthweight preterm infants and to evaluate the impact of perinatal factors, such as delivery mode and perinatal antibiotics. STUDY DESIGN: We used 16S ribosomal RNA gene sequence-based microbiota analysis and quantitative polymerase chain reaction to evaluate the establishment of the intestinal microbiota. We also evaluated factors affecting the microbiota, during the first 3 months of life in preterm infants (n = 27) compared with full-term babies (n = 13). RESULTS: Immaturity affects the microbiota as indicated by a reduced percentage of the family Bacteroidaceae during the first months of life and by a higher initial percentage of Lactobacillaceae in preterm infants compared with full term infants. Perinatal antibiotics, including intrapartum antimicrobial prophylaxis, affects the gut microbiota, as indicated by increased Enterobacteriaceae family organisms in the infants. CONCLUSIONS: Prematurity and perinatal antibiotic administration strongly affect the initial establishment of microbiota with potential consequences for later health.

Experiment 1


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Folakunmi, Victoria

Subjects

Location of subjects
Spain
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Premature birth Birth, Premature,Birth, Preterm,Births, Premature,Births, Preterm,Premature Births,Preterm Birth,Preterm Births,Premature birth,premature birth
Group 0 name Corresponds to the control (unexposed) group for case-control studies
2-day old Full-term infants(born after uncomplicated pregnancy)
Group 1 name Corresponds to the case (exposed) group for case-control studies
2-day old Pre-term infants
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
infants born at gestational ages between 24 and 32 weeks
Group 0 sample size Number of subjects in the control (unexposed) group
13
Group 1 sample size Number of subjects in the case (exposed) group
27

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Ion Torrent

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
ANOVA
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-19

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Results within text under the subheading "Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants" Line 7 to 15

Description: Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants

Abundance in Group 1: increased abundance in 2-day old Pre-term infants

NCBI Quality ControlLinks
Bifidobacteriaceae
Comamonadaceae
Propionibacteriaceae
Streptococcaceae
unclassified Actinomycetota
unclassified Bacilli
unclassified Lactobacillales

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-20

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Results within text under the subheading "Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants" (Line 7 to 15)

Description: Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants

Abundance in Group 1: decreased abundance in 2-day old Pre-term infants

NCBI Quality ControlLinks
Bacteroidaceae
Clostridiaceae
Micrococcaceae
Pasteurellaceae
Porphyromonadaceae

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 2


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
10-day old Full-term infants(born after uncomplicated pregnancy)
Group 1 name Corresponds to the case (exposed) group for case-control studies
10-day old Pre-term
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
born at gestational ages between 24 and 32 weeks

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-20

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Results within text under the subheading "Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants" (line 15 to 22)

Description: Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants

Abundance in Group 1: increased abundance in 10-day old Pre-term

NCBI Quality ControlLinks
Enterobacteriaceae
Gammaproteobacteria
Micrococcaceae

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-20

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, Atrayees, ChiomaBlessing

Source: Results within text under the subheading "Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants" (line 15 to 22)

Description: Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants

Abundance in Group 1: decreased abundance in 10-day old Pre-term

NCBI Quality ControlLinks
Veillonellaceae
Bacteroidaceae
Bifidobacteriaceae
Clostridiaceae
Coriobacteriaceae
Lactobacillaceae
Pasteurellaceae
Porphyromonadaceae
unclassified Actinomycetota

Revision editor(s): WikiWorks, Atrayees, ChiomaBlessing

Experiment 3


Needs review Marked as Needs review by Lwaldron on 2023-11-16

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees, Folakunmi, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
30 and 90-day old Full-term infants
Group 1 name Corresponds to the case (exposed) group for case-control studies
30 and 90-day old Pre-term infants
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
infants born at gestational ages between 24 and 32 weeks

Lab analysis

Statistical Analysis

Signature 1

Needs review Marked as Needs review by Lwaldron on 2023-11-16

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, Folakunmi

Source: Results within text under the subheading "Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants" (line 23 to 26)

Description: Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants

Abundance in Group 1: increased abundance in 30 and 90-day old Pre-term infants

NCBI Quality ControlLinks
Enterobacteriaceae

Revision editor(s): WikiWorks, Folakunmi

Signature 2

Needs review Marked as Needs review by Lwaldron on 2023-11-16

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, Folakunmi

Source: Results within text under the subheading "Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants" (line 23 to 26)

Description: Establishment of Intestinal Microbiota in VLBW Preterm Neonates Compared with FTVDBF Infants

Abundance in Group 1: decreased abundance in 30 and 90-day old Pre-term infants

NCBI Quality ControlLinks
Bacteroidaceae

Revision editor(s): WikiWorks, Folakunmi

Experiment 4


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2023/11/20

Curator: Folakunmi

Revision editor(s): Folakunmi, Victoria

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Delivery method DeliveryMethod,Delivery method,delivery method
Group 0 name Corresponds to the control (unexposed) group for case-control studies
10-day old cesarean-delivered pre-term neontes
Group 1 name Corresponds to the case (exposed) group for case-control studies
10-day old vaginally-delivered pre-term neonates
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
10-day old pre-term infants who were delivered vaginally.
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
7

Lab analysis

Sequencing type
PCR
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-20

Curated date: 2023/11/20

Curator: Folakunmi

Revision editor(s): Folakunmi

Source: Results within text under the sub-heading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota" (Line 6-8)

Description: Impact of Delivery method on the Intestinal Microbiota between 10-day old vaginally-delivered pre-term neonates and cesarean-delivered pre-term neonates, as observed by qPCR.

Abundance in Group 1: increased abundance in 10-day old vaginally-delivered pre-term neonates

NCBI Quality ControlLinks
Bacteroides

Revision editor(s): Folakunmi

Experiment 5


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Antimicrobial agent antibiotic,antibiotics,Antibiotika,Antibiotikum,antibiotique,antimicrobial,antimicrobial agents,microbicide,microbicides,Antimicrobial agent,antimicrobial agent
Group 0 name Corresponds to the control (unexposed) group for case-control studies
2-day old full-term neonates not exposed to antibiotics (IAP/postnatal)
Group 1 name Corresponds to the case (exposed) group for case-control studies
2-day old pre-tem neonates exposed to antibiotics (IAP)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
antenatal/postnatal antibiotic exposure
Group 0 sample size Number of subjects in the control (unexposed) group
14
Group 1 sample size Number of subjects in the case (exposed) group
10

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, Folakunmi, ChiomaBlessing

Source: Results within text under the subheading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota" (2nd paragraph, from line 11)

Description: Impact of Antibiotics Use on the Intestinal Microbiota between 2-day old full-term neonates not exposed to antibiotics (IAP/postnatal) and 2-day old pre-term neonates exposed to antibiotics (IAP)

Abundance in Group 1: decreased abundance in 2-day old pre-tem neonates exposed to antibiotics (IAP)

NCBI Quality ControlLinks
Lactobacillaceae

Revision editor(s): WikiWorks, Folakunmi, ChiomaBlessing

Experiment 6


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
10-day old full-term neonates not exposed to antibiotics (IAP/postnatal)
Group 1 name Corresponds to the case (exposed) group for case-control studies
10-day old pre-tem neonates exposed to antibiotics (IAP)

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Results within text under the subheading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota" (2nd paragraph, from line 11)

Description: Impact of Antibiotics Use on the Intestinal Microbiota between 10-day old full-term neonates not exposed to antibiotics (IAP/postnatal) and 10-day old pre-tem neonates exposed to antibiotics (IAP)

Abundance in Group 1: decreased abundance in 10-day old pre-tem neonates exposed to antibiotics (IAP)

NCBI Quality ControlLinks
Micrococcaceae
Propionibacteriaceae

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 7


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
30-day old full-term neonates not exposed to antibiotics (IAP/postnatal)
Group 1 name Corresponds to the case (exposed) group for case-control studies
30-day old pre-tem neonates exposed to antibiotics (IAP)

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, Atrayees, ChiomaBlessing

Source: Results within text under the subheading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota" (2nd paragraph, from line 18)

Description: Impact of Antibiotics Use on the Intestinal Microbiota between 30-day old full-term neonates not exposed to antibiotics (IAP/postnatal) and 30-day old pre-term neonates exposed to antibiotics (IAP)

Abundance in Group 1: decreased abundance in 30-day old pre-tem neonates exposed to antibiotics (IAP)

NCBI Quality ControlLinks
Comamonadaceae
Staphylococcaceae
unclassified Bacilli

Revision editor(s): WikiWorks, Atrayees, ChiomaBlessing

Experiment 8


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Folakunmi, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
90-day old full-term neonates not exposed to antibiotics (IAP/postnatal)
Group 1 name Corresponds to the case (exposed) group for case-control studies
90-day old pre-term neonates with mothers exposed to antibiotics (IAP)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
90-day old pre-term neonates whose mothers received antibiotics (IAP) independently on whether or not the infant received antibiotics.

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Results within text under the subheading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota" (2nd paragraph, from line 31)

Description: Impact of Antibiotics Use on the Intestinal Microbiota between 90-day old full-term neonates not exposed to antibiotics (IAP/postnatal) and 90-day old pre-term neonates with mothers exposed to antibiotics (IAP)

Abundance in Group 1: decreased abundance in 90-day old pre-term neonates with mothers exposed to antibiotics (IAP)

NCBI Quality ControlLinks
Oscillospiraceae

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 9


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Folakunmi, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
30-day old full-term neonates not exposed to antibiotics (IAP/postnatal)
Group 1 name Corresponds to the case (exposed) group for case-control studies
30-day old pre-tem neonates with mothers exposed to antibiotics (IAP)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
30-day old pre-term neonates whose mothers received antibiotics (IAP) independently on whether or not the infant received antibiotics.
Group 0 sample size Number of subjects in the control (unexposed) group
5
Group 1 sample size Number of subjects in the case (exposed) group
14

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-20

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Results within text under the subheading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota" (2nd paragraph, from line 24)

Description: Impact of Antibiotics Use on the Intestinal Microbiota between 30-day old full-term neonates not exposed to antibiotics (IAP/postnatal) and 30-day old pre-term neonates with mothers exposed to antibiotics (IAP)

Abundance in Group 1: increased abundance in 30-day old pre-tem neonates with mothers exposed to antibiotics (IAP)

NCBI Quality ControlLinks
Enterobacteriaceae

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-20

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Results within text under the subheading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota" (2nd paragraph, from line 20)

Description: Impact of Antibiotics Use on the Intestinal Microbiota between 30-day old full-term neonates not exposed to antibiotics (IAP/postnatal) and 30-day old pre-term neonates with mothers exposed to antibiotics (IAP)

Abundance in Group 1: decreased abundance in 30-day old pre-tem neonates with mothers exposed to antibiotics (IAP)

NCBI Quality ControlLinks
Bifidobacteriaceae
Streptococcaceae
unclassified Lactobacillales
unclassified Actinomycetota

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 10


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
30-day old pre-term neonates not exposed to antibiotics (IAP/postnatal)
Group 1 name Corresponds to the case (exposed) group for case-control studies
30-day old pre-tem neonates exposed to antibiotics (IAP/postnatal)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
pre-term infants and mothers exposed to antibiotics
Group 1 sample size Number of subjects in the case (exposed) group
9

Lab analysis

Sequencing type
PCR
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-20

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, Atrayees, ChiomaBlessing

Source: Results for qtPCR within text under the subheading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota", 5th paragraph)

Description: Impact of Antibiotics Use on the Intestinal Microbiota between 30-day old pre-term neonates not exposed to antibiotics (IAP/postnatal) and 30-day old pre-term neonates exposed to antibiotics (IAP/postnatal)

Abundance in Group 1: decreased abundance in 30-day old pre-tem neonates exposed to antibiotics (IAP/postnatal)

NCBI Quality ControlLinks
Staphylococcaceae

Revision editor(s): WikiWorks, Atrayees, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-20

Curated date: 2023/11/20

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Results for qtPCR within text under the subheading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota", 5th paragraph)

Description: Impact of Antibiotics Use on the Intestinal Microbiota between 30-day old pre-term neonates not exposed to antibiotics (IAP/postnatal) and 30-day old pre-term neonates exposed to antibiotics (IAP/postnatal)

Abundance in Group 1: increased abundance in 30-day old pre-tem neonates exposed to antibiotics (IAP/postnatal)

NCBI Quality ControlLinks
Enterobacteriaceae

Revision editor(s): ChiomaBlessing

Experiment 11


Reviewed Marked as Reviewed by Atrayees on 2023-7-21

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
90-day old pre-term neonates not exposed to antibiotics (IAP/postnatal)
Group 1 name Corresponds to the case (exposed) group for case-control studies
90-day old pre-tem neonates exposed to antibiotics (IAP/postnatal)

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-11-20

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Results for qtPCR within text under the subheading "Impact of Delivery Mode and Antibiotics Use on the Establishment of Intestinal Microbiota" (5th paragraph)

Description: Impact of Antibiotics Use on the Intestinal Microbiota between 90-day old pre-term neonates not exposed to antibiotics (IAP/postnatal) and 90-day old pre-term neonates exposed to antibiotics (IAP/postnatal)

Abundance in Group 1: decreased abundance in 90-day old pre-tem neonates exposed to antibiotics (IAP/postnatal)

NCBI Quality ControlLinks
Bifidobacterium

Revision editor(s): WikiWorks, ChiomaBlessing