Exposure to concentrated ambient PM_2.5 alters the composition of gut microbiota in a murine model

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/12/29

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

29665823

DOI Digital object identifier for electronic documents.

10.1186/s12989-018-0252-6

URI

Authors

Wang W, Zhou J, Chen M, Huang X, Xie X, Li W, Cao Q, Kan H, Xu Y, Ying Z

Journal

Particle and fibre toxicology

Year

2018

Keywords:

Diabetes, Glucose homeostatic, Gut microbiota, PM2.5

BACKGROUND: Exposure to ambient fine particulate matter (PM2.5) correlates with abnormal glucose homeostasis, but the underlying biological mechanism has not been fully understood. The gut microbiota is an emerging crucial player in the homeostatic regulation of glucose metabolism. Few studies have investigated its role in the PM2.5 exposure-induced abnormalities in glucose homeostasis. METHODS: C57Bl/6J mice were exposed to filtered air (FA) or concentrated ambient PM2.5 (CAP) for 12 months using a versatile aerosol concentration enrichment system (VACES) that was modified for long-term whole-body exposures. Their glucose homeostasis and gut microbiota were examined and analysed by correlation and mediation analysis. RESULTS: Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) showed that CAP exposure markedly impaired their glucose and insulin tolerance. Faecal microbiota analysis demonstrated that the impairment in glucose homeostasis was coincided with decreased faecal bacterial ACE and Chao-1 estimators (the indexes of community richness), while there was no significant change in all faecal fungal alpha diversity estimators. The Pearson's correlation analyses showed that the bacterial richness estimators were correlated with glucose and insulin tolerance, and the mediation analyses displayed a significant mediation of CAP exposure-induced glucose intolerance by the alteration in the bacterial Chao-1 estimator. LEfSe analyses revealed 24 bacterial and 21 fungal taxa differential between CAP- and FA-exposed animals. Of these, 14 and 20 bacterial taxa were correlated with IPGTT AUC and ITT AUC, respectively, and 5 fungal taxa were correlated with abnormalities in glucose metabolism. CONCLUSIONS: Chronic exposure to PM2.5 causes gut dysbiosis and may subsequently contribute to the development of abnormalities in glucose metabolism.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/12/29

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Air pollution air pollution,Air pollution

Group 0 name Corresponds to the control (unexposed) group for case-control studies: mice exposed to PM 2.5 (CAP)

Group 1 name Corresponds to the case (exposed) group for case-control studies: mice exposed to filtered air (FA)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: male C57Bl/6 J mice exposed to PM2.5 (CAP)

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 10

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: ANOVA

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Chao1 Abundance-based estimator of species richness: increased

Simpson Estimator of species richness and species evenness: more weight on species evenness: increased

Richness Number of species: unchanged

Signature 1

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Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure 4 & text

Description: The differential taxa between FA- and CAP-exposed mice

Abundance in Group 1: increased abundance in mice exposed to filtered air (FA)

NCBI	Quality Control	Links
Marvinbryantia
Mycoplasma
Mycoplasmataceae
Mycoplasmatales
Rikenellaceae
Alistipes finegoldii
Metamycoplasma sualvi
Rikenella microfusus
Lachnospiraceae bacterium DW52

Revision editor(s): Claregrieve1, WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/12/29

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure 4 & text

Description: The differential taxa between FA- and CAP-exposed mice

Abundance in Group 1: decreased abundance in mice exposed to filtered air (FA)

NCBI	Quality Control	Links
Campylobacterales
Campylobacterota
Helicobacter
Helicobacteraceae
Peptostreptococcaceae
Romboutsia
Clostridium sp. 001
Clostridiaceae
Lachnospiraceae bacterium 10-1
Clostridium sp. Culture Jar-56
Papillibacter
Allobaculum
Turicibacter
Helicobacter hepaticus