Decreased bacterial diversity characterizes the altered gut microbiota in patients with psoriatic arthritis, resembling dysbiosis in inflammatory bowel disease

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/12

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

25319745

DOI Digital object identifier for electronic documents.

10.1002/art.38892

URI

Authors

Scher JU, Ubeda C, Artacho A, Attur M, Isaac S, Reddy SM, Marmon S, Neimann A, Brusca S, Patel T, Manasson J, Pamer EG, Littman DR, Abramson SB

Journal

Arthritis & rheumatology (Hoboken, N.J.)

Year

2015

OBJECTIVE: To characterize the diversity and taxonomic relative abundance of the gut microbiota in patients with never-treated, recent-onset psoriatic arthritis (PsA). METHODS: High-throughput 16S ribosomal RNA pyrosequencing was utilized to compare the community composition of gut microbiota in patients with PsA (n = 16), patients with psoriasis of the skin (n = 15), and healthy, matched control subjects (n = 17). Samples were further assessed for the presence and levels of fecal and serum secretory IgA (sIgA), proinflammatory proteins, and fatty acids. RESULTS: The gut microbiota observed in patients with PsA and patients with skin psoriasis was less diverse when compared to that in healthy controls. This could be attributed to the reduced presence of several taxa. Samples from both patient groups showed a relative decrease in abundance of Coprococcus species, while samples from PsA patients were also characterized by a significant reduction in Akkermansia, Ruminococcus, and Pseudobutyrivibrio. Supernatants of fecal samples from PsA patients revealed an increase in sIgA levels and decrease in RANKL levels. Analysis of fatty acids revealed low fecal quantities of hexanoate and heptanoate in both patients with PsA and patients with psoriasis. CONCLUSION: Patients with PsA and patients with skin psoriasis had a lower relative abundance of multiple intestinal bacteria. Although some genera were concomitantly decreased in both conditions, PsA samples had a lower abundance of reportedly beneficial taxa. This gut microbiota profile in PsA was similar to that previously described in patients with inflammatory bowel disease and was associated with changes in specific inflammatory proteins unique to this group, and distinct from that in patients with skin psoriasis and healthy controls. Thus, the role of the gut microbiome in the continuum of psoriasis-PsA pathogenesis and the associated immune response merits further study.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/12

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Psoriatic arthritis Arthritic Psoriasis,arthritis psoriatica,Arthritis, Psoriatic,arthropathic psoriasis,Psoriasis Arthropathica,Psoriasis, Arthritic,psoriatic arthritis,psoriatic arthritis, susceptibility to,psoriatic arthritis, susceptibility to, 1,psoriatic arthropathy,susceptibility to psoriatic arthritis,Psoriatic arthritis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: psoriatic arthiritis

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: patients fulfilling the criteria of the Classification of Psoriatic arthritis (CASPAR)

Group 0 sample size Number of subjects in the control (unexposed) group: 17

Group 1 sample size Number of subjects in the case (exposed) group: 16

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 3 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V2

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.2

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Matched on Factors on which subjects have been matched on in a case-control study: age, ethnic group, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Signature 1

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Source: Figure 1a, Figure 2a

Description: Differences in bacterial taxa between patients with psoriatic arthritis (PsA) compared to healthy controls (HLT)

Abundance in Group 1: decreased abundance in psoriatic arthiritis

NCBI	Quality Control	Links
Akkermansia
Clostridia
Coprococcus
Pseudobutyrivibrio
Ruminococcus
Verrucomicrobiota
Verrucomicrobiia
Verrucomicrobiales

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Experiment 2

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Curated date: 2021/01/10

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Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: skin psoriasis

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: patients fulfilling the skin psoriasis criteria of the Classification of Psoriatic arthritis (CASPAR)

Group 1 sample size Number of subjects in the case (exposed) group: 15

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Signature 1

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Curated date: 2021/01/10

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Source: Figure 1b, Figure 2b

Description: Differences in bacterial taxa between patients with skin psoriasis (PS) compared to healthy controls (HLT)

Abundance in Group 1: decreased abundance in skin psoriasis

NCBI	Quality Control	Links
Porphyromonadaceae
Parabacteroides
Clostridia
Erysipelotrichaceae
Erysipelotrichia
Actinomycetes
Actinomycetota
Coprobacillus
Erysipelotrichales
Coprococcus
Lachnospiraceae
Oscillospiraceae
Bacteroidota

Revision editor(s): WikiWorks

Experiment 3

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/12

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: skin psoriasis

Group 1 name Corresponds to the case (exposed) group for case-control studies: psoriatic arthiritis

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: patients fulfilling the psoriatic arthritis criteria of the Classification of Psoriatic arthritis (CASPAR)