Alterations of the Gut Microbiota in Hashimoto's Thyroiditis Patients

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Reviewed Marked as Reviewed by Atrayees on 2023-7-19
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Zhao F, Feng J, Li J, Zhao L, Liu Y, Chen H, Jin Y, Zhu B, Wei Y
Journal
Thyroid : official journal of the American Thyroid Association
Year
2018
Keywords:
Hashimoto's thyroiditis, biomarker, clinical parameters, dysbiosis, gut microbiota
BACKGROUND: Hashimoto's thyroiditis (HT) is an organ-specific autoimmune disease in which both genetic predisposition and environmental factors serve as disease triggers. Many studies have indicated that alterations in the gut microbiota are important environmental factors in the development of inflammatory and autoimmune diseases. A comparative analysis was systematically performed of the gut microbiota in HT patients and healthy controls. METHODS: First, a cross-sectional study of 28 HT patients and 16 matched healthy controls was conducted. Fecal samples were collected, and microbiota were analyzed using 16S ribosomal RNA gene sequencing. Second, an independent cohort of 22 HT patients and 11 healthy controls was used to evaluate the diagnostic potential of the selected biomarkers. RESULTS: Similar levels of bacterial richness and diversity were found in the gut microbiota of HT patients and healthy controls (p = 0.11). A detailed fecal microbiota Mann-Whitney U-test (Q value <0.05) revealed that the abundance levels of Blautia, Roseburia, Ruminococcus_torques_group, Romboutsia, Dorea, Fusicatenibacter, and Eubacterium_hallii_group genera were increased in HT patients, whereas the abundance levels of Fecalibacterium, Bacteroides, Prevotella_9, and Lachnoclostridium genera were decreased. A correlation matrix based on the Spearman correlation distance confirmed correlations among seven clinical parameters. Additionally, the linear discriminant analysis effect size method showed significant differences in 27 genera between the two groups that were strongly correlated with clinical parameters. The linear discriminant analysis value was used to select the first 10 species from the 27 different genera as biomarkers, achieving area under the curve values of 0.91 and 0.88 for exploration and validation data, respectively. CONCLUSIONS: Characterization of the gut microbiota in HT patients confirmed that HT patients have altered gut microbiota and that gut microbiota are correlated with clinical parameters, suggesting that microbiome composition data could be used for disease diagnosis. Further investigation is required to understand better the role of the gut microbiota in the pathogenesis of HT.

Experiment 1


Reviewed Marked as Reviewed by Atrayees on 2023-7-19

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Hashimoto's thyroiditis autoimmune thyroiditis,chronic lymphocytic thyroiditides,chronic lymphocytic thyroiditis,disease, Hashimoto,disease, Hashimoto's,Hashimoto disease,Hashimoto struma,Hashimoto syndrome,Hashimoto thyroiditides,Hashimoto Thyroiditis,Hashimoto thyroiditis,Hashimoto's disease,Hashimoto's struma,Hashimoto's syndrome,Hashimoto's syndromes,Hashimoto's thyroiditis,Hashimotos disease,Hashimotos syndrome,HT,Ht,hypothyroidism, autoimmune,hypothyroidism, autoimmune thyroid autoantibodies, included,Lymphocytic Thyroiditides,lymphocytic thyroiditides, chronic,Lymphocytic Thyroiditis,lymphocytic thyroiditis,lymphocytic thyroiditis, chronic,Lymphomatous Thyroiditides,syndrome, Hashimoto's,syndromes, Hashimoto's,thyroid autoantibodies,thyroiditides, chronic lymphocytic,thyroiditides, Hashimoto,Thyroiditides, Lymphocytic,Thyroiditides, Lymphomatous,thyroiditis, chronic lymphocytic,thyroiditis, Hashimoto,Thyroiditis, Lymphocytic,Thyroiditis, Lymphomatous,hashimoto's thyroiditis
Group 0 name Corresponds to the control (unexposed) group for case-control studies
healthy controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
hashimoto's thyroiditis patients
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
presence of euthyroidism, free thyroxine, and thyrotropin without hormonal therapy
Group 0 sample size Number of subjects in the control (unexposed) group
27
Group 1 sample size Number of subjects in the case (exposed) group
50
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3
Matched on Factors on which subjects have been matched on in a case-control study
age, body mass index, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-19

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure 2a, 2b, table 3

Description: Comparisons of the relative abundance using LEfSe analysis

Abundance in Group 1: increased abundance in hashimoto's thyroiditis patients

NCBI Quality ControlLinks
Anaerobutyricum hallii
Anaerostipes
Blautia
Butyricicoccus
Clostridiaceae
Coprococcus
Dorea
Erysipelotrichaceae
Eubacterium ruminantium
Bacillota
Fusicatenibacter
Haemophilus
Lachnospiraceae
Pasteurellaceae
Peptostreptococcaceae
Romboutsia
Roseburia
Ruminococcus gauvreauii
Streptococcaceae
Streptococcus
Subdoligranulum
[Ruminococcus] torques
Clostridium

Revision editor(s): Claregrieve1, WikiWorks

Signature 2

Reviewed Marked as Reviewed by Atrayees on 2023-7-19

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 2a, 2b, table 3

Description: Comparisons of the relative abundance using LEfSe analysis

Abundance in Group 1: decreased abundance in hashimoto's thyroiditis patients

NCBI Quality ControlLinks
Bacteroides
Faecalibacterium
Prevotella
Alloprevotella
Phascolarctobacterium
Lachnoclostridium
Parabacteroides
Paraprevotella
Gaiella
Bacteroidaceae
Prevotellaceae
Porphyromonadaceae
Acidaminococcaceae
Gaiellaceae
Bacteroidota
Synergistota

Revision editor(s): WikiWorks