Molecular estimation of alteration in intestinal microbial composition in Hashimoto's thyroiditis patients

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

28903182

DOI Digital object identifier for electronic documents.

10.1016/j.biopha.2017.08.101

URI

Authors

Ishaq HM, Mohammad IS, Guo H, Shahzad M, Hou YJ, Ma C, Naseem Z, Wu X, Shi P, Xu J

Journal

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Year

2017

Keywords:

DGGE, Gut microbiota, Hashimoto’s thyroiditis, Hyporthyroidism, Pyrosequencing

The gut microbiota has a crucial effect on human health and physiology. Hypothyroid Hashimoto's thyroiditis (HT) is an autoimmune disorder manifested with environmental and genetic factors. However, it is hypothesized that intestinal microbes might play a vital role in the pathogenesis of HT. The aim of current was to investigate and characterize the gut microbial composition of HT patients both quantitatively and qualitatively. The fecal samples from 29 HT patients and 12 healthy individuals were collected. The PCR-DGGE targeted V3 site of 16S rRNA gene and real time PCR for Bifidobacterium Lactobacillus, Bacteroides vulgatus and Clostridium leptum were performed. Pyrosequencing of 16S rRNA gene with V4 location was performed on 20 randomly selected samples. The comparative analysis of diversity and richness indices revealed diversification of gut microbiota in HT as compared to control. The statistical data elucidate the alterations in phyla of HT patients which was also affirmed at the family level. We observed the declined abundance of Prevotella_9 and Dialister, while elevated genera of the diseased group included Escherichia-Shigella and Parasutterella. The alteration in gut microbial configuration was also monitored at the species level, which showed an increased abundance of E. coli in HT. Therefore, the current study is in agreement with the hypothesis that HT patients have intestinal microbial dysbiosis. The taxa statistics at species-level along with each gut microbial community were modified in HT. Thus, the current study may offer the new insights into the treatment of HT patients, disease pathway, and mechanism.

Experiment 1

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Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Hashimoto's thyroiditis autoimmune thyroiditis,chronic lymphocytic thyroiditides,chronic lymphocytic thyroiditis,disease, Hashimoto,disease, Hashimoto's,Hashimoto disease,Hashimoto struma,Hashimoto syndrome,Hashimoto thyroiditides,Hashimoto Thyroiditis,Hashimoto thyroiditis,Hashimoto's disease,Hashimoto's struma,Hashimoto's syndrome,Hashimoto's syndromes,Hashimoto's thyroiditis,Hashimotos disease,Hashimotos syndrome,HT,Ht,hypothyroidism, autoimmune,hypothyroidism, autoimmune thyroid autoantibodies, included,Lymphocytic Thyroiditides,lymphocytic thyroiditides, chronic,Lymphocytic Thyroiditis,lymphocytic thyroiditis,lymphocytic thyroiditis, chronic,Lymphomatous Thyroiditides,syndrome, Hashimoto's,syndromes, Hashimoto's,thyroid autoantibodies,thyroiditides, chronic lymphocytic,thyroiditides, Hashimoto,Thyroiditides, Lymphocytic,Thyroiditides, Lymphomatous,thyroiditis, chronic lymphocytic,thyroiditis, Hashimoto,Thyroiditis, Lymphocytic,Thyroiditis, Lymphomatous,hashimoto's thyroiditis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: hashimoto's thyroiditis patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: The Thyroid stimulating hormone(TSH) was higher than 5 μIU/ml, T4 was lower than 4.2 μg/dl, T3 was lower than 0.78 ng/ml, anti-thyroid peroxidase antibodies level was higher than 15 IU/ml, and anti-thyroglobulin antibodies was more than 30%

Group 0 sample size Number of subjects in the control (unexposed) group: 12

Group 1 sample size Number of subjects in the case (exposed) group: 29

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 60 days

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: T-Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: increased

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

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Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Table 7

Description: Hashimoto's thyroid gut microbial phylotypes from pyrosequencing

Abundance in Group 1: increased abundance in hashimoto's thyroiditis patients

NCBI	Quality Control	Links
Ruminococcus flavefaciens

Revision editor(s): WikiWorks

Signature 2

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Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Table 7

Description: Hashimoto's thyroid gut microbial phylotypes from pyrosequencing

Abundance in Group 1: decreased abundance in hashimoto's thyroiditis patients

NCBI	Quality Control	Links
Lactobacillus gasseri

Revision editor(s): WikiWorks