Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-24

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

23638009

DOI Digital object identifier for electronic documents.

10.1371/journal.pone.0062220

URI

Authors

Kish L, Hotte N, Kaplan GG, Vincent R, Tso R, Gänzle M, Rioux KP, Thiesen A, Barkema HW, Wine E, Madsen KL

Journal

PloS one

Year

2013

BACKGROUND: Particulate matter (PM) is a key pollutant in ambient air that has been associated with negative health conditions in urban environments. The aim of this study was to examine the effects of orally administered PM on the gut microbiome and immune function under normal and inflammatory conditions. METHODS: Wild-type 129/SvEv mice were gavaged with Ottawa urban PM10 (EHC-93) for 7-14 days and mucosal gene expression analyzed using Ingenuity Pathways software. Intestinal permeability was measured by lactulose/mannitol excretion in urine. At sacrifice, segments of small and large intestine were cultured and cytokine secretion measured. Splenocytes were isolated and incubated with PM10 for measurement of proliferation. Long-term effects of exposure (35 days) on intestinal cytokine expression were measured in wild-type and IL-10 deficient (IL-10(-/-)) mice. Microbial composition of stool samples was assessed using terminal restriction fragment length polymorphism. Short chain fatty acids were measured in caecum. RESULTS: Short-term treatment of wild-type mice with PM10 altered immune gene expression, enhanced pro-inflammatory cytokine secretion in the small intestine, increased gut permeability, and induced hyporesponsiveness in splenocytes. Long-term treatment of wild-type and IL-10(-/-) mice increased pro-inflammatory cytokine expression in the colon and altered short chain fatty acid concentrations and microbial composition. IL-10(-/-) mice had increased disease as evidenced by enhanced histological damage. CONCLUSIONS: Ingestion of airborne particulate matter alters the gut microbiome and induces acute and chronic inflammatory responses in the intestine.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-24

Curated date: 2021/01/10

Curator: WikiWorks

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Subjects

Location of subjects: Canada

Host species Species from which microbiome was sampled (if applicable): Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: air pollution air pollution

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy mice
Group 1 name Corresponds to the case (exposed) group for case-control studies: Chronic treatment group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: 6-week old female wild-type 129/SvEv mice in chronic treatment group were fed mouse chow ± PM10 (0.09 gm/kg) for 35 days
Group 0 sample size Number of subjects in the control (unexposed) group: 7
Group 1 sample size Number of subjects in the case (exposed) group: 8

Lab analysis

Sequencing type: 16S

Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Non-quantitative PCR

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

PLS-DA (Partial least square discriminant analysis)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Signature 1

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Curated date: 2021/01/10

Curator: Zyaijah Bailey

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Source: Figure 6

Description: Microbiota composition in stool samples from WT and IL-10−/− mice after 35 days of treatment with PM10.

Abundance in Group 1: increased abundance in Chronic treatment group

NCBI	Quality Control	Links
Verrucomicrobiota

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Experiment 2

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Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: IL-10−/− mice fed mouse chow with PM10
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: IL-10−/− mice in chronic treatment group were fed mouse chow ± PM10 (0.09 gm/kg) for 35 days
Group 0 sample size Number of subjects in the control (unexposed) group: 9
Group 1 sample size Number of subjects in the case (exposed) group: 7