Ambient Ultrafine Particle Ingestion Alters Gut Microbiota in Association with Increased Atherogenic Lipid Metabolites

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

28211537

DOI Digital object identifier for electronic documents.

10.1038/srep42906

URI

Authors

Li R, Yang J, Saffari A, Jacobs J, Baek KI, Hough G, Larauche MH, Ma J, Jen N, Moussaoui N, Zhou B, Kang H, Reddy S, Henning SM, Campen MJ, Pisegna J, Li Z, Fogelman AM, Sioutas C, Navab M, Hsiai TK

Journal

Scientific reports

Year

2017

Ambient particulate matter (PM) exposure is associated with atherosclerosis and inflammatory bowel disease. Ultrafine particles (UFP, dp < 0.1-0.2 μm) are redox active components of PM. We hypothesized that orally ingested UFP promoted atherogenic lipid metabolites in both the intestine and plasma via altered gut microbiota composition. Low density lipoprotein receptor-null (Ldlr-/-) mice on a high-fat diet were orally administered with vehicle control or UFP (40 μg/mouse/day) for 3 days a week. After 10 weeks, UFP ingested mice developed macrophage and neutrophil infiltration in the intestinal villi, accompanied by elevated cholesterol but reduced coprostanol levels in the cecum, as well as elevated atherogenic lysophosphatidylcholine (LPC 18:1) and lysophosphatidic acids (LPAs) in the intestine and plasma. At the phylum level, Principle Component Analysis revealed significant segregation of microbiota compositions which was validated by Beta diversity analysis. UFP-exposed mice developed increased abundance in Verrocomicrobia but decreased Actinobacteria, Cyanobacteria, and Firmicutes as well as a reduced diversity in microbiome. Spearman's analysis negatively correlated Actinobacteria with cecal cholesterol, intestinal and plasma LPC18:1, and Firmicutes and Cyanobacteria with plasma LPC 18:1. Thus, ultrafine particles ingestion alters gut microbiota composition, accompanied by increased atherogenic lipid metabolites. These findings implicate the gut-vascular axis in a atherosclerosis model.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, ChiomaBlessing

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Caecum Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Air pollution air pollution,Air pollution

Group 0 name Corresponds to the control (unexposed) group for case-control studies: vehicle control

Group 1 name Corresponds to the case (exposed) group for case-control studies: mice exposed to UFP

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: C57BL/6 mice exposed to UFP (Ultrafine particles)

Group 0 sample size Number of subjects in the control (unexposed) group: 11

Group 1 sample size Number of subjects in the case (exposed) group: 12

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: ANOVA

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Chao1 Abundance-based estimator of species richness: decreased

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure 2 & Supplemental Figure S1& Text

Description: Differential microbial abundance between UFP-exposed mice and controls

Abundance in Group 1: increased abundance in mice exposed to UFP

NCBI	Quality Control	Links
Verrucomicrobiota

Revision editor(s): Claregrieve1, WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure 2 & Supplemental Figure S1& Text

Description: Differential microbial abundance between UFP-exposed mice and controls

Abundance in Group 1: decreased abundance in mice exposed to UFP

NCBI	Quality Control	Links
Actinomycetota
Cyanobacteriota
Bacillota

Revision editor(s): Claregrieve1, WikiWorks