Effects of PM2.5 exposure during gestation on maternal gut microbiota and pregnancy outcomes
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Liu W, Zhou Y, Yong Li Y, Qin L, Yu R, Li Y, Chen Y, Xu
Journal
Chemosphere
Year
2020
Keywords:
Gut microbiota, PM(2.5), Pregnancy outcomes, Short chain fatty acids
A number of studies have reported that fine particulate matter (PM2.5) exposure is associated with adverse pregnancy outcomes. Moreover, PM2.5 exposure contributes to changes of gut microbiota. However, influences of PM2.5 exposure during gestation on maternal gut microbiota and pregnancy outcomes were not well understood. Here we performed a study using mice models. Dams were exposed to PM2.5 suspension by intratracheal instillation on gestational day (GD) 3, 6, 9, 12 and 15. Pregnancy outcomes, maternal gut microbiota and short chain fatty acids on GD 18 were all measured. The fetal body weight of PM2.5 group was significantly lower than that of control group (p < 0.05). Meanwhile, the fetal body length of PM2.5 group was significantly shorter than that of control group (p < 0.05). The Shannon or Simpson index of PM2.5 group were higher than that of control group (p < 0.05). At the phyla level, compared to dams in control group, mice in the PM2.5 group had higher ratio of phyla Proteobacteria, Candidatus Saccharibacteria and Fusobacteria and lower ratio of phyla Acidobacteria, Gemmatimonadetes and Deferribacteres in the gut. Compared with control group, the concentration of isobutyric acid was higher in PM2.5 group, but butyric acid concentration was lower in PM2.5 group (p < 0.05). These findings suggested that prenatal exposure to PM2.5 had an effect on birth weight of fetus. Meanwhile, PM2.5 tracheal exposure during gestation caused changes in the distribution and structure of gut microbiota of dams.
Experiment 1
Reviewed Marked as Reviewed by Atrayees on 2023-7-3
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Colon Hindgut,Large bowel,Posterior intestine,Colon,colon
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Air pollution air pollution,Air pollution
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- mice exposed suspension from extracts of “blank” filter
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- mice exposed to Pm 2.5 suspension
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- exposed to PM2.5 suspension
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
- Group 1 sample size Number of subjects in the case (exposed) group
- 12
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 2
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-7-3
Source: Figure 4a, text
Description: the most differentially abundant taxa between the two groups were identified through the LDA score which was generated from LEfSe analysis (phylum to genus: p, phylum; c, class; o, order; f, family; g, genus)
Abundance in Group 1: increased abundance in mice exposed to Pm 2.5 suspension
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-7-3
Source: Figure 4a, text
Description: the most differentially abundant taxa between the two groups were identified through the LDA score which was generated from LEfSe analysis (phylum to genus: p, phylum; c, class; o, order; f, family; g, genus)
Abundance in Group 1: decreased abundance in mice exposed to Pm 2.5 suspension
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