Effects of PM_2.5 exposure during gestation on maternal gut microbiota and pregnancy outcomes

Study information

Reviewed Marked as Reviewed by Atrayees on 2023-7-3

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

31935575

DOI Digital object identifier for electronic documents.

10.1016/j.chemosphere.2020.125879

URI

Authors

Liu W, Zhou Y, Yong Li Y, Qin L, Yu R, Li Y, Chen Y, Xu

Journal

Chemosphere

Year

2020

Keywords:

Gut microbiota, PM(2.5), Pregnancy outcomes, Short chain fatty acids

A number of studies have reported that fine particulate matter (PM2.5) exposure is associated with adverse pregnancy outcomes. Moreover, PM2.5 exposure contributes to changes of gut microbiota. However, influences of PM2.5 exposure during gestation on maternal gut microbiota and pregnancy outcomes were not well understood. Here we performed a study using mice models. Dams were exposed to PM2.5 suspension by intratracheal instillation on gestational day (GD) 3, 6, 9, 12 and 15. Pregnancy outcomes, maternal gut microbiota and short chain fatty acids on GD 18 were all measured. The fetal body weight of PM2.5 group was significantly lower than that of control group (p < 0.05). Meanwhile, the fetal body length of PM2.5 group was significantly shorter than that of control group (p < 0.05). The Shannon or Simpson index of PM2.5 group were higher than that of control group (p < 0.05). At the phyla level, compared to dams in control group, mice in the PM2.5 group had higher ratio of phyla Proteobacteria, Candidatus Saccharibacteria and Fusobacteria and lower ratio of phyla Acidobacteria, Gemmatimonadetes and Deferribacteres in the gut. Compared with control group, the concentration of isobutyric acid was higher in PM2.5 group, but butyric acid concentration was lower in PM2.5 group (p < 0.05). These findings suggested that prenatal exposure to PM2.5 had an effect on birth weight of fetus. Meanwhile, PM2.5 tracheal exposure during gestation caused changes in the distribution and structure of gut microbiota of dams.

Experiment 1

Edit History Delete

Flag for review

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Atrayees on 2023-7-3

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Colon Hindgut,Large bowel,Posterior intestine,Colon,colon

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Air pollution air pollution,Air pollution

Group 0 name Corresponds to the control (unexposed) group for case-control studies: mice exposed suspension from extracts of “blank” filter

Group 1 name Corresponds to the case (exposed) group for case-control studies: mice exposed to Pm 2.5 suspension

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: exposed to PM2.5 suspension

Group 0 sample size Number of subjects in the control (unexposed) group: 12

Group 1 sample size Number of subjects in the case (exposed) group: 12

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 2

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: increased

Richness Number of species: unchanged

Signature 1

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Lwaldron, WikiWorks, Merit

Source: Figure 4a, text

Description: the most differentially abundant taxa between the two groups were identified through the LDA score which was generated from LEfSe analysis (phylum to genus: p, phylum; c, class; o, order; f, family; g, genus)

Abundance in Group 1: increased abundance in mice exposed to Pm 2.5 suspension

NCBI	Quality Control	Links
Deltaproteobacteria
Desulfovibrionaceae
Desulfovibrionales
Flavonifractor
Fusobacteriaceae
Fusobacteriales
Fusobacteriia
Fusobacteriota
Fusobacterium
Oscillibacter
Pseudomonadota
candidate phylum NAG2
Oscillibacter sp.
Fusobacterium sp.

Revision editor(s): Lwaldron, WikiWorks, Merit

Signature 2

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Atrayees, Merit

Source: Figure 4a, text

Description: the most differentially abundant taxa between the two groups were identified through the LDA score which was generated from LEfSe analysis (phylum to genus: p, phylum; c, class; o, order; f, family; g, genus)

Abundance in Group 1: decreased abundance in mice exposed to Pm 2.5 suspension

NCBI	Quality Control	Links
Acidobacteriota
Azotobacter group
Bacillales
Bacilli
Bacillus
Deferribacteraceae
Deferribacterales
Deferribacteres
Deferribacterota
Delftia
Gammaproteobacteria
Gemmatimonadaceae
Gemmatimonadales
Gemmatimonadia
Gemmatimonadota
Gemmatimonas
Leucobacter
Microbacteriaceae
Mucispirillum
Odoribacter
Pseudomonadales
Pseudomonas
Selenomonas
Sphingomonadales
Sphingomonas
Streptococcaceae
Streptococcus
Odoribacteraceae
Streptococcus sp.