Effects of PM2.5 exposure during gestation on maternal gut microbiota and pregnancy outcomes

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Reviewed Marked as Reviewed by Atrayees on 2023-7-3
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Liu W, Zhou Y, Yong Li Y, Qin L, Yu R, Li Y, Chen Y, Xu
Journal
Chemosphere
Year
2020
Keywords:
Gut microbiota, PM(2.5), Pregnancy outcomes, Short chain fatty acids
A number of studies have reported that fine particulate matter (PM2.5) exposure is associated with adverse pregnancy outcomes. Moreover, PM2.5 exposure contributes to changes of gut microbiota. However, influences of PM2.5 exposure during gestation on maternal gut microbiota and pregnancy outcomes were not well understood. Here we performed a study using mice models. Dams were exposed to PM2.5 suspension by intratracheal instillation on gestational day (GD) 3, 6, 9, 12 and 15. Pregnancy outcomes, maternal gut microbiota and short chain fatty acids on GD 18 were all measured. The fetal body weight of PM2.5 group was significantly lower than that of control group (p < 0.05). Meanwhile, the fetal body length of PM2.5 group was significantly shorter than that of control group (p < 0.05). The Shannon or Simpson index of PM2.5 group were higher than that of control group (p < 0.05). At the phyla level, compared to dams in control group, mice in the PM2.5 group had higher ratio of phyla Proteobacteria, Candidatus Saccharibacteria and Fusobacteria and lower ratio of phyla Acidobacteria, Gemmatimonadetes and Deferribacteres in the gut. Compared with control group, the concentration of isobutyric acid was higher in PM2.5 group, but butyric acid concentration was lower in PM2.5 group (p < 0.05). These findings suggested that prenatal exposure to PM2.5 had an effect on birth weight of fetus. Meanwhile, PM2.5 tracheal exposure during gestation caused changes in the distribution and structure of gut microbiota of dams.

Experiment 1


Reviewed Marked as Reviewed by Atrayees on 2023-7-3

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon,colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Air pollution air pollution,Air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice exposed suspension from extracts of “blank” filter
Group 1 name Corresponds to the case (exposed) group for case-control studies
mice exposed to Pm 2.5 suspension
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
exposed to PM2.5 suspension
Group 0 sample size Number of subjects in the control (unexposed) group
12
Group 1 sample size Number of subjects in the case (exposed) group
12

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
2
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-3

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Lwaldron, WikiWorks, Merit

Source: Figure 4a, text

Description: the most differentially abundant taxa between the two groups were identified through the LDA score which was generated from LEfSe analysis (phylum to genus: p, phylum; c, class; o, order; f, family; g, genus)

Abundance in Group 1: increased abundance in mice exposed to Pm 2.5 suspension

NCBI Quality ControlLinks
Deltaproteobacteria
Desulfovibrionaceae
Desulfovibrionales
Flavonifractor
Fusobacteriaceae
Fusobacteriales
Fusobacteriia
Fusobacteriota
Fusobacterium
Oscillibacter
Pseudomonadota
candidate phylum NAG2
Oscillibacter sp.
Fusobacterium sp.

Revision editor(s): Lwaldron, WikiWorks, Merit

Signature 2

Reviewed Marked as Reviewed by Atrayees on 2023-7-3

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Atrayees, Merit

Source: Figure 4a, text

Description: the most differentially abundant taxa between the two groups were identified through the LDA score which was generated from LEfSe analysis (phylum to genus: p, phylum; c, class; o, order; f, family; g, genus)

Abundance in Group 1: decreased abundance in mice exposed to Pm 2.5 suspension

NCBI Quality ControlLinks
Acidobacteriota
Azotobacter group
Bacillales
Bacilli
Bacillus
Deferribacteraceae
Deferribacterales
Deferribacteres
Deferribacterota
Delftia
Gammaproteobacteria
Gemmatimonadaceae
Gemmatimonadales
Gemmatimonadia
Gemmatimonadota
Gemmatimonas
Leucobacter
Microbacteriaceae
Mucispirillum
Odoribacter
Pseudomonadales
Pseudomonas
Selenomonas
Sphingomonadales
Sphingomonas
Streptococcaceae
Streptococcus
Odoribacteraceae
Streptococcus sp.

Revision editor(s): WikiWorks, Atrayees, Merit