Impacts of Dietary Protein and Niacin Deficiency on Reproduction Performance, Body Growth, and Gut Microbiota of Female Hamsters (Tscherskia triton) and Their Offspring
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Curated date: 2024/03/24
Curator: Aleru Divine
Revision editor(s): Aleru Divine, Victoria, Scholastica
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Tscherskia triton
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Female hamsters Normal-protein diet (NPD) group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Female hamsters Low-protein diet (LPD) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Female hamsters under low-protein diet LPD
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- centered log-ratio
- Statistical test
- ANCOM
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Signature 1
Source: Table S5 and Figure 5A
Description: Significant differential abundance in fecal microbiota of female hamster in Normal-protein diet (NPD) versus low-protein diet (LPD).
Abundance in Group 1: increased abundance in Female hamsters Low-protein diet (LPD) group
NCBI | Quality Control | Links |
---|---|---|
Anaeroplasma | ||
Bacteroides | ||
Dysgonomonas | ||
Georgfuchsia | ||
unclassified Bacillaceae | ||
ChristensenellaceaeChristensenellaceae |
Revision editor(s): Aleru Divine, KateRasheed
Signature 2
Source: Table S5 and Figure 5A
Description: Significant differential abundance in fecal microbiota of female hamster in Normal-protein diet (NPD) versus low-protein diet (LPD).
Abundance in Group 1: decreased abundance in Female hamsters Low-protein diet (LPD) group
Revision editor(s): Aleru Divine
Experiment 3
Curated date: 2024/03/24
Curator: Aleru Divine
Revision editor(s): Aleru Divine, Victoria, Scholastica
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Offspring hamsters Normal-protein diet (NPD) group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Offspring hamsters Low-protein diet (LPD) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Offspring hamsters under low-protein diet LPD
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Table S6 and Figure 5C
Description: Significant differential abundance in fecal microbiota of offspring hamster in Normal-protein diet (NPD) versus low-protein diet (LPD).
Abundance in Group 1: increased abundance in Offspring hamsters Low-protein diet (LPD) group
Revision editor(s): Aleru Divine
Signature 2
Source: Table S6 and Figure 5C
Description: Significant differential abundance in fecal microbiota of offspring hamster in Normal-protein diet (NPD) versus low-protein diet (LPD).
Abundance in Group 1: decreased abundance in Offspring hamsters Low-protein diet (LPD) group
Revision editor(s): Aleru Divine
Experiment 4
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Offspring hamsters naicin+ group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Offspring hamsters niacin- group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Offspring hamsters under niacin-deficient (niacin-) diet.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Table S7 and Figure 5B
Description: Significant differential abundance in fecal microbiota of offspring hamster in niacin-supplemented diet (Niacin+) versus niacin-deficient diet (Niacin−).
Abundance in Group 1: increased abundance in Offspring hamsters niacin- group
NCBI | Quality Control | Links |
---|---|---|
unclassified Actinomycetales | ||
Spirochaeta | ||
Proteiniborus |
Revision editor(s): Aleru Divine
Signature 2
Source: Table S7 and Figure 5B
Description: Significant differential abundance in fecal microbiota of offspring hamster in niacin-supplemented diet (Niacin+) versus niacin-deficient diet (Niacin−).
Abundance in Group 1: decreased abundance in Offspring hamsters niacin- group
NCBI | Quality Control | Links |
---|---|---|
Alkanindiges | ||
Arthrobacter | ||
Hespellia | ||
Macrococcus | ||
Mucispirillum | ||
unclassified Comamonadaceae | ||
unclassified Paraprevotella | ||
unclassified Porphyromonadaceae | ||
Clostridium |
Revision editor(s): Aleru Divine