Impacts of Dietary Protein and Niacin Deficiency on Reproduction Performance, Body Growth, and Gut Microbiota of Female Hamsters (Tscherskia triton) and Their Offspring
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Zhao J, Lu W, Huang S, Le Maho Y, Habold C, Zhang Z
Journal
Microbiology spectrum
Year
2022
Keywords:
cropland monoculture, greater long-tailed hamster, gut microbiota, low-protein diet, niacin deficiency
Food resources are vital for animals to survive, and gut microbiota play an essential role in transferring nutritional materials into functional metabolites for hosts. Although the fact that diet affects host microbiota is well known, its impacts on offspring remain unclear. In this study, we assessed the effects of low-protein and niacin-deficient diets on reproduction performance, body growth, and gut microbiota of greater long-tailed hamsters (Tscherskia triton) under laboratory conditions. We found that maternal low-protein diet (not niacin deficiency) had a significant negative effect on reproduction performance of female hamsters (longer mating latency with males and smaller litter size) and body growth (lower body weight) of both female hamsters and their offspring. Both protein- and niacin-deficient diets showed significant maternal effects on the microbial community in the offspring. A maternal low-protein diet (not niacin deficiency) significantly reduced the abundance of major bacterial taxa producing short-chain fatty acids, increased the abundance of probiotic taxa, and altered microbial function in the offspring. The negative effects of maternal nutritional deficiency on gut microbiota are more pronounced in the protein group than the niacin group and in offspring more than in female hamsters. Our results suggest that a low-protein diet could alter gut microbiota in animals, which may result in negative impacts on their fitness. It is necessary to conduct further analysis to reveal the roles of nutrition, as well as its interaction with gut microbes, in affecting fitness of greater long-tailed hamsters under field conditions. IMPORTANCE Gut microbes are known to be essential for hosts to digest food and absorb nutrients. Currently, it is still unclear how maternal nutrient deficiency affects the fitness of animals by its effect on gut microbes. Here, we evaluated the effects of protein- and niacin-deficient diets on mating behavior, reproduction, body growth, and gut microbiota of both mothers and offspring of the greater long-tailed hamster (Tscherskia triton) under laboratory conditions. We found that a low-protein diet significantly reduced maternal reproduction performance and body growth of both mothers and their offspring. Both protein and niacin deficiencies showed significant maternal effects on the microbial community of the offspring. Our results hint that nutritional deficiency may be a potential factor in causing the observed sustained population decline of the greater long-tailed hamsters due to intensified monoculture in the North China Plain, and this needs further field investigation.
Experiment 1
Reviewed Marked as Reviewed by Peace Sandy on 2024-2-8
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Tscherskia triton
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Female hamsters NPD group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Female hamsters LPD group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Female hamsters under low-protein diet LPD
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- ANCOM
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Signature 1
Reviewed Marked as Reviewed by Peace Sandy on 2024-2-8
Source: Table S5 and Figure 5A
Description: Significant differential abundance in fecal microbiota of female hamster in Normal-protein diet (NPD) versus low-protein diet (LPD).
Abundance in Group 1: increased abundance in Female hamsters LPD group
| NCBI | Quality Control | Links |
|---|---|---|
| Anaeroplasma | ||
| Bacteroides | ||
| Dysgonomonas | ||
| Georgfuchsia | ||
| unclassified Bacillaceae | ||
| unclassified Fusobacteriaceae | ||
| unclassified Pseudomonadota | ||
| unclassified Veillonellaceae | ||
| YRC22YRC22 |
Revision editor(s): Aleru Divine
Signature 2
Reviewed Marked as Reviewed by Peace Sandy on 2024-2-8
Source: Table S5 and Figure 5A
Description: Significant differential abundance in fecal microbiota of female hamster in Normal-protein diet (NPD) versus low-protein diet (LPD).
Abundance in Group 1: decreased abundance in Female hamsters LPD group
Revision editor(s): Aleru Divine
Experiment 2
Needs review
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Female hamsters naicin+ group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Female hamsters niacin- group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Female hamsters under niacin-deficient (niacin-) diet.
Lab analysis
Statistical Analysis
Experiment 3
Needs review
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Offspring hamsters NPD group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Offspring hamsters LPD group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Offspring hamsters under low-protein diet LPD
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Needs review
Source: Table S6 and Figure 5C
Description: Significant differential abundance in fecal microbiota of offspring hamster in Normal-protein diet (NPD) versus low-protein diet (LPD).
Abundance in Group 1: increased abundance in Offspring hamsters LPD group
Revision editor(s): Aleru Divine
Signature 2
Needs review
Source: Table S6 and Figure 5C
Description: Significant differential abundance in fecal microbiota of offspring hamster in Normal-protein diet (NPD) versus low-protein diet (LPD).
Abundance in Group 1: decreased abundance in Offspring hamsters LPD group
Revision editor(s): Aleru Divine
Experiment 4
Needs review
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Offspring hamsters naicin+ group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Offspring hamsters niacin- group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Offspring hamsters under niacin-deficient (niacin-) diet.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Needs review
Source: Table S7 and Figure 5B
Description: Significant differential abundance in fecal microbiota of offspring hamster in niacin-supplemented diet (Niacin+) versus niacin-deficient diet (Niacin−).
Abundance in Group 1: increased abundance in Offspring hamsters niacin- group
| NCBI | Quality Control | Links |
|---|---|---|
| unclassified Actinomycetales | ||
| Spirochaeta | ||
| Proteiniborus |
Revision editor(s): Aleru Divine
Signature 2
Needs review
Source: Table S7 and Figure 5B
Description: Significant differential abundance in fecal microbiota of offspring hamster in niacin-supplemented diet (Niacin+) versus niacin-deficient diet (Niacin−).
Abundance in Group 1: decreased abundance in Offspring hamsters niacin- group
| NCBI | Quality Control | Links |
|---|---|---|
| Alkanindiges | ||
| Arthrobacter | ||
| Hespellia | ||
| Macrococcus | ||
| Mucispirillum | ||
| unclassified Comamonadaceae | ||
| unclassified Paraprevotella | ||
| unclassified Porphyromonadaceae | ||
| Clostridium |
Revision editor(s): Aleru Divine
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