Correlation between body mass index and faecal microbiota from children
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Ignacio A, Fernandes MR, Rodrigues VA, Groppo FC, Cardoso AL, Avila-Campos MJ, Nakano V
Journal
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
Year
2016
Keywords:
Anaerobic bacteria, Escherichia coli, body mass index, childhood obesity, faecal microbiota, quantitative PCR
Childhood obesity is an increasing problem at the global level and considered as a risk factor for obesity development and the associated co-morbidities in adult life. In this study, the occurrence of Bacteroides fragilis group, Clostridium spp., Bifidobacterium spp. and Escherichia coli in 84 faecal samples from 30 obese, 24 overweight and 30 lean children was verified by culture technique and quantitative determination by quantitative PCR. In addition, Lactobacillus spp. and Methanobrevibacter smithii were also analysed. A correlation between the body mass index (BMI) and these bacteria was sought. Bacteroides vulgatus, Clostridium perfringens and Bifidobacterium adolescentis were most prevalent in all samples evaluated by culture-method. The B. fragilis group were found at high concentrations in obese and overweight children when compared with the lean ones (p 0.015). The obese and overweight children harboured higher numbers of Lactobacillus spp. than lean children (p 0.022). The faecal concentrations of the B. fragilis group (r = 0.24; p 0.026) and Lactobacillus spp. (r = 0.44; p 0.002) were positively correlated with BMI. Bifidobacterium spp. were found in higher numbers in the lean group than the overweight and obese ones (p 0.042). Furthermore, a negative correlation between BMI and Bifidobacterium spp. copy number (r = -0.22; p 0.039) was observed. Our findings show some difference in the intestinal microbial ecosystem of obese children compared with the lean ones and a significant association between number of Lactobacillus spp. and B. fragilis group and BMI.
Experiment 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/26
Subjects
- Location of subjects
- Brazil
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- lean children
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- obese children
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy children with obesity based on z-score of >+2
- Group 0 sample size Number of subjects in the control (unexposed) group
- 30
- Group 1 sample size Number of subjects in the case (exposed) group
- 30
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- RT-qPCR
Statistical Analysis
- Statistical test
- Kruskall-Wallis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/27
Source: Table 4
Description: Bacterial prevalence and quantification verified in faeces of obese, overweight and lean children by quantitative PCR
Abundance in Group 1: increased abundance in obese children
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides fragilis | ||
| Lactobacillus sp. |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/27
Source: Table 4
Description: Bacterial prevalence and quantification verified in faeces of obese, overweight and lean children by quantitative PCR
Abundance in Group 1: decreased abundance in obese children
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium sp. |
Revision editor(s): WikiWorks
Experiment 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/27
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- overweight children
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy children who are considered overweight based on z-score of ≥ +1 and < +2
- Group 1 sample size Number of subjects in the case (exposed) group
- 24
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/27
Source: Table 4
Description: Bacterial prevalence and quantification verified in faeces of obese, overweight and lean children by quantitative PCR
Abundance in Group 1: increased abundance in overweight children
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides fragilis | ||
| Lactobacillus sp. |
Revision editor(s): WikiWorks
Experiment 3
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/27
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- obese children
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy children with obesity based on z-score of >+2
- Group 1 sample size Number of subjects in the case (exposed) group
- 30
Lab analysis
Statistical Analysis
- Statistical test
- Logistic Regression
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/27
Source: Table 6
Description: Factors associated with body mass index based on multiple logistic regression (logistic regression analysis using quantitative PCR results
Abundance in Group 1: increased abundance in obese children
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides fragilis |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/27
Source: Table 6
Description: Factors associated with body mass index based on multiple logistic regression (logistic regression analysis using quantitative PCR results
Abundance in Group 1: decreased abundance in obese children
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium sp. |
Revision editor(s): WikiWorks
Experiment 4
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/27
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- overweight children
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy children who are considered overweight based on z-score of ≥ +1 and < +2
- Group 1 sample size Number of subjects in the case (exposed) group
- 24
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/27
Source: Table 6
Description: Factors associated with body mass index based on multiple logistic regression (logistic regression analysis using quantitative PCR results
Abundance in Group 1: increased abundance in overweight children
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus sp. |
Revision editor(s): Claregrieve1, WikiWorks
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