Cervical microbiota in women with cervical intra-epithelial neoplasia, prior to and after local excisional treatment, a Norwegian cohort study

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Wiik J, Sengpiel V, Kyrgiou M, Nilsson S, Mitra A, Tanbo T, Monceyron Jonassen C, Møller Tannæs T, Sjøborg K
Journal
BMC women's health
Year
2019
Keywords:
CIN, Cervical Intraepithelial Neoplasia, HPV, Human papillomavirus, LEEP, Lactobacillus, Vaginal microbiota
BACKGROUND: Local treatment for cervical intraepithelial neoplasia (CIN) by Loop Electrosurgical Excision Procedure (LEEP) has been correlated with reproductive morbidity, while the cervicovaginal microbiota is also known to affect the risk of preterm delivery. CIN and treatment by LEEP might change the cervical microbiota. The main aim of this study was to describe the cervical microbiota before and after LEEP and assess its associaton with cone depth and HPV persistence. Further, we aimed to compare the microbiota to references with normal cervical cytology. METHODS: Between 2005 and 2007, we prospectively identified 89 women planned for LEEP in a Norwegian hospital and recruited 100 references with a normal cervical cytology. Endocervical swabs were collected prior to treatment and at six (n = 77) and 12 months (n = 72) post LEEP for bacterial culture and PCR, and post LEEP for DNA testing for human papillomavirus (HPV). We compared the cervical microbiota composition before and after treatment and between women planned for LEEP vs references. RESULTS: There was a reduction in the number of non-Lactobacillus bacterial species six and 12 months after LEEP compared to before treatment and a tendency towards a concomitant increase in Lactobacillus. No association between the detection of cervical bacteria, HPV persistence or cone depth was found. Women planned for LEEP carried significantly more Bacteroides spp., Gardnerella vaginalis, Mycoplasma hominis and Ureaplasma parvum as well as a greater number of bacterial species than the references. CONCLUSIONS: Local excisional treatment appears to alter the cervical microbiota towards a less diverse microbiota. Women with CIN have a more diverse cervical microbiota compared to women with normal cervical cytology.

Experiment 1


Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Subjects

Location of subjects
Norway
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Endocervix Endocervix,endocervix
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Cervical glandular intraepithelial neoplasia Cervical glandular intraepithelial neoplasia,cervical glandular intraepithelial neoplasia
Group 0 name Corresponds to the control (unexposed) group for case-control studies
normal
Group 1 name Corresponds to the case (exposed) group for case-control studies
LEEP group before procedure
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
women with suspected high grade cervical dysplasia planning for their first Loop Electrosurgical Excision Procedure
Group 0 sample size Number of subjects in the control (unexposed) group
100
Group 1 sample size Number of subjects in the case (exposed) group
89
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
long-term antibiotic use

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
RT-qPCR

Statistical Analysis

Statistical test
Fisher's Exact Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Claregrieve1, WikiWorks

Source: Table 5

Description: Differential abundance of cervical microbiota in the LEEP group and the Reference group

Abundance in Group 1: increased abundance in LEEP group before procedure

NCBI Quality ControlLinks
Gardnerella vaginalis
Ureaplasma parvum
Metamycoplasma hominis

Revision editor(s): Claregrieve1, WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 sample size Number of subjects in the control (unexposed) group
99

Lab analysis

Statistical Analysis

Statistical test
Logistic Regression
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
age, contraception, smoking behavior, marital status


Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Claregrieve1, WikiWorks

Source: Table 5

Description: Differential abundance of cervical microbiota in the LEEP group and the Reference group

Abundance in Group 1: increased abundance in LEEP group before procedure

NCBI Quality ControlLinks
Gardnerella vaginalis
Ureaplasma parvum

Revision editor(s): Claregrieve1, WikiWorks

Experiment 3


Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
LEEP group before procedure (age <46)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
women <46 years old with suspected high grade cervical dysplasia planning for their first Loop Electrosurgical Excision Procedure
Group 0 sample size Number of subjects in the control (unexposed) group
48
Group 1 sample size Number of subjects in the case (exposed) group
75

Lab analysis

Statistical Analysis

Statistical test
Fisher's Exact Test
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
Not specified


Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplemental Table 1

Description: Differential abundance of the cervical microbiota in the LEEP group and the Reference group in women aged < 46 years

Abundance in Group 1: increased abundance in LEEP group before procedure (age <46)

NCBI Quality ControlLinks
Gardnerella vaginalis
Metamycoplasma hominis
Ureaplasma parvum

Revision editor(s): Claregrieve1, WikiWorks

Experiment 4


Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
LEEP group before procedure

Lab analysis

Statistical Analysis

Statistical test
Logistic Regression
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
age, contraception, smoking behavior, marital status


Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplemental Table 1

Description: Differential abundance of cervical microbiota in the LEEP group and the Reference group in women aged < 46 years

Abundance in Group 1: increased abundance in LEEP group before procedure

NCBI Quality ControlLinks
Gardnerella vaginalis
Ureaplasma parvum

Revision editor(s): Claregrieve1, WikiWorks

Experiment 5


Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
LEEP group 6 months post-procedure
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
women 6 months after Loop Electrosurgical Excision Procedure
Group 0 sample size Number of subjects in the control (unexposed) group
100
Group 1 sample size Number of subjects in the case (exposed) group
77

Lab analysis

Statistical Analysis

Statistical test
Fisher's Exact Test
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
Not specified


Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplemental Table 2

Description: Differential abundance between the cervical microbiota six months post treatment in the LEEP group and the cervical microbiota in the Reference group

Abundance in Group 1: increased abundance in LEEP group 6 months post-procedure

NCBI Quality ControlLinks
Gardnerella vaginalis
Metamycoplasma hominis
Ureaplasma parvum

Revision editor(s): Claregrieve1, WikiWorks

Experiment 6


Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
LEEP group 12 months post-procedure
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
women 12 months after Loop Electrosurgical Excision Procedure
Group 1 sample size Number of subjects in the case (exposed) group
72

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplemental Table 3

Description: Differential abundance between cervical microbiota 12 months post treatment in the LEEP group and the cervical microbiota in the Reference group

Abundance in Group 1: increased abundance in LEEP group 12 months post-procedure

NCBI Quality ControlLinks
Gardnerella vaginalis
Metamycoplasma hominis
Ureaplasma parvum

Revision editor(s): Claregrieve1, WikiWorks