Cervical microbiota in women with cervical intra-epithelial neoplasia, prior to and after local excisional treatment, a Norwegian cohort study
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Wiik J, Sengpiel V, Kyrgiou M, Nilsson S, Mitra A, Tanbo T, Monceyron Jonassen C, Møller Tannæs T, Sjøborg K
Journal
BMC women's health
Year
2019
Keywords:
CIN, Cervical Intraepithelial Neoplasia, HPV, Human papillomavirus, LEEP, Lactobacillus, Vaginal microbiota
BACKGROUND: Local treatment for cervical intraepithelial neoplasia (CIN) by Loop Electrosurgical Excision Procedure (LEEP) has been correlated with reproductive morbidity, while the cervicovaginal microbiota is also known to affect the risk of preterm delivery. CIN and treatment by LEEP might change the cervical microbiota. The main aim of this study was to describe the cervical microbiota before and after LEEP and assess its associaton with cone depth and HPV persistence. Further, we aimed to compare the microbiota to references with normal cervical cytology. METHODS: Between 2005 and 2007, we prospectively identified 89 women planned for LEEP in a Norwegian hospital and recruited 100 references with a normal cervical cytology. Endocervical swabs were collected prior to treatment and at six (n = 77) and 12 months (n = 72) post LEEP for bacterial culture and PCR, and post LEEP for DNA testing for human papillomavirus (HPV). We compared the cervical microbiota composition before and after treatment and between women planned for LEEP vs references. RESULTS: There was a reduction in the number of non-Lactobacillus bacterial species six and 12 months after LEEP compared to before treatment and a tendency towards a concomitant increase in Lactobacillus. No association between the detection of cervical bacteria, HPV persistence or cone depth was found. Women planned for LEEP carried significantly more Bacteroides spp., Gardnerella vaginalis, Mycoplasma hominis and Ureaplasma parvum as well as a greater number of bacterial species than the references. CONCLUSIONS: Local excisional treatment appears to alter the cervical microbiota towards a less diverse microbiota. Women with CIN have a more diverse cervical microbiota compared to women with normal cervical cytology.
Experiment 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Subjects
- Location of subjects
- Norway
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Endocervix Endocervix,endocervix
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Cervical glandular intraepithelial neoplasia Cervical glandular intraepithelial neoplasia,cervical glandular intraepithelial neoplasia
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- normal
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- LEEP group before procedure
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- women with suspected high grade cervical dysplasia planning for their first Loop Electrosurgical Excision Procedure
- Group 0 sample size Number of subjects in the control (unexposed) group
- 100
- Group 1 sample size Number of subjects in the case (exposed) group
- 89
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- long-term antibiotic use
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- RT-qPCR
Statistical Analysis
- Statistical test
- Fisher's Exact Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Source: Table 5
Description: Differential abundance of cervical microbiota in the LEEP group and the Reference group
Abundance in Group 1: increased abundance in LEEP group before procedure
| NCBI | Quality Control | Links |
|---|---|---|
| Gardnerella vaginalis | ||
| Ureaplasma parvum | ||
| Metamycoplasma hominis |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Differences from previous experiment shown
Subjects
- Group 0 sample size Number of subjects in the control (unexposed) group
- 99
Lab analysis
Statistical Analysis
- Statistical test
- Logistic Regression
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, contraception, smoking behavior, marital status
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Source: Table 5
Description: Differential abundance of cervical microbiota in the LEEP group and the Reference group
Abundance in Group 1: increased abundance in LEEP group before procedure
| NCBI | Quality Control | Links |
|---|---|---|
| Gardnerella vaginalis | ||
| Ureaplasma parvum |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 3
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- LEEP group before procedure (age <46)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- women <46 years old with suspected high grade cervical dysplasia planning for their first Loop Electrosurgical Excision Procedure
- Group 0 sample size Number of subjects in the control (unexposed) group
- 48
- Group 1 sample size Number of subjects in the case (exposed) group
- 75
Lab analysis
Statistical Analysis
- Statistical test
- Fisher's Exact Test
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- Not specified
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Source: Supplemental Table 1
Description: Differential abundance of the cervical microbiota in the LEEP group and the Reference group in women aged < 46 years
Abundance in Group 1: increased abundance in LEEP group before procedure (age <46)
| NCBI | Quality Control | Links |
|---|---|---|
| Gardnerella vaginalis | ||
| Metamycoplasma hominis | ||
| Ureaplasma parvum |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 4
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- LEEP group before procedure
Lab analysis
Statistical Analysis
- Statistical test
- Logistic Regression
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, contraception, smoking behavior, marital status
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Source: Supplemental Table 1
Description: Differential abundance of cervical microbiota in the LEEP group and the Reference group in women aged < 46 years
Abundance in Group 1: increased abundance in LEEP group before procedure
| NCBI | Quality Control | Links |
|---|---|---|
| Gardnerella vaginalis | ||
| Ureaplasma parvum |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 5
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- LEEP group 6 months post-procedure
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- women 6 months after Loop Electrosurgical Excision Procedure
- Group 0 sample size Number of subjects in the control (unexposed) group
- 100
- Group 1 sample size Number of subjects in the case (exposed) group
- 77
Lab analysis
Statistical Analysis
- Statistical test
- Fisher's Exact Test
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- Not specified
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Source: Supplemental Table 2
Description: Differential abundance between the cervical microbiota six months post treatment in the LEEP group and the cervical microbiota in the Reference group
Abundance in Group 1: increased abundance in LEEP group 6 months post-procedure
| NCBI | Quality Control | Links |
|---|---|---|
| Gardnerella vaginalis | ||
| Metamycoplasma hominis | ||
| Ureaplasma parvum |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 6
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- LEEP group 12 months post-procedure
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- women 12 months after Loop Electrosurgical Excision Procedure
- Group 1 sample size Number of subjects in the case (exposed) group
- 72
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/28
Source: Supplemental Table 3
Description: Differential abundance between cervical microbiota 12 months post treatment in the LEEP group and the cervical microbiota in the Reference group
Abundance in Group 1: increased abundance in LEEP group 12 months post-procedure
| NCBI | Quality Control | Links |
|---|---|---|
| Gardnerella vaginalis | ||
| Metamycoplasma hominis | ||
| Ureaplasma parvum |
Revision editor(s): Claregrieve1, WikiWorks
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