Overweight and Obesity in Children Are Associated with an Abundance of Firmicutes and Reduction of Bifidobacterium in Their Gastrointestinal Microbiota

Study information

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study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

31934770

DOI Digital object identifier for electronic documents.

10.1089/chi.2019.0280

URI

Authors

Da Silva CC, Monteil MA, Davis EM

Journal

Childhood obesity (Print)

Year

2020

Keywords:

bifidobacterium, children, firmicutes, gut, microbiome, obesity

Background: Obesity in children continues to be a global epidemic. Given the recent focus on the potential role gut microbiota play in the development of obesity and the continuous rise of obesity in childhood worldwide, it has become necessary that the association between gut microbes and weight is explored in previously unexplored regions such as the Caribbean island of Trinidad. As such, our objective was to characterize the composition of the gut microbiota in children with obesity/overweight and children of normal weight to determine if there are any gut microbes associated with overweight and obesity. Methods: Fifty-one children provided stool samples and their BMIs were calculated and classified. 16S amplicons from stool samples were individually barcoded and sequenced in multiplex in the NextSeq 500 platform in a 150 bp paired-end modality and a complete gut microbiome profile was generated. Results: The relative abundance and diversity of the microbiota of the lean and children with obesity/overweight were analyzed against each other and showed that children with obesity/overweight recorded significantly more (p = 0.001) Firmicutes and significantly less Bifidobacterium (p = 0.039) than the children of normal weight. Children with obesity/overweight also recorded significantly (p = 0.009) reduced alpha diversity compared with the lean children. Conclusion: This study has shown associations of Firmicutes to obesity/overweight and Bifidobacterium species with healthy weight in children.

Experiment 1

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Curated date: 2021/01/10

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Subjects

Location of subjects: Trinidad and Tobago

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity

Group 0 name Corresponds to the control (unexposed) group for case-control studies: normal

Group 1 name Corresponds to the case (exposed) group for case-control studies: overweight/obesity

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: school aged children (6-14 years)

Group 0 sample size Number of subjects in the control (unexposed) group: 30

Group 1 sample size Number of subjects in the case (exposed) group: 21

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 3 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: ANOVA

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Richness Number of species: decreased

Signature 1

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Curated date: 2021/01/10

Curator: Mst Afroza Parvin

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Source: Table 2 & 3

Description: The Relative Abundance (%) of the Most Dominant Bacterial Families & genus from Stool Samples at Varying Demographic and Anthropometric Measure

Abundance in Group 1: increased abundance in overweight/obesity

NCBI	Quality Control	Links
Lactobacillus

Revision editor(s): WikiWorks

Signature 2

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): WikiWorks

Source: Table 2 & 3

Description: The Relative Abundance (%) of the Most Dominant Bacterial Families & genus from Stool Samples at Varying Demographic and Anthropometric Measure

Abundance in Group 1: decreased abundance in overweight/obesity

NCBI	Quality Control	Links
Bifidobacteriaceae
Bifidobacterium

Revision editor(s): WikiWorks