Sex-specific effects of PM2.5 maternal exposure on offspring's serum lipoproteins and gut microbiota

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8
study design
laboratory experiment
Citation
PMID PubMed identifier for scientific articles.
32544691
DOI Digital object identifier for electronic documents.
10.1016/j.scitotenv.2020.139982
URI
Authors
Liu W, Zhou Y, Qin Y, Li Y, Yu L, Li R, Chen Y, Xu Y
Journal
The Science of the total environment
Year
2020
Keywords:
Gut microbiota, Lipoproteins, Offspring, PM(2.5), Sex-specific
Fine particulate matter (PM2.5), which is known to impact public health, has received widespread attention recently. However, the long-term impact of maternal PM2.5 exposure remains unclear. To illuminate whether maternal PM2.5 exposure can affect serum lipoproteins and intestinal flora of offspring, mice received PM2.5 by intratracheal instillation during gestation and lactation. On postnatal day (PND) 35, serum lipoproteins of male and female pups were measured. Additionally, gut microbiota of offspring on PND 3, 10, 21 and 35 were measured by 16S rDNA sequencing of the colon contents. A higher serum triglyceride (TG) concentration in male offspring was observed in the exposed PM2.5 group (p < 0.05) compared with the control group, while there was no significant difference in lipoproteins for female offspring. On PND 35, Bacteroides, Desulfovibrio, and Anaerotruncus were enriched in the male offspring of the PM2.5-exposed group, and the control group had an increased abundance of Streptococcus. However, for female offspring on PND35, Clostridium XI was found to be enriched in the control group. A positive correlation between Bacteroides and serum TG concentration (r = 0.47, p = 0.02) was determined by Spearman's correlation analysis. These results suggest that serum TG and gut microbiota of offspring could be influenced by maternal PM2.5 exposure in a sex-specific manner. Abnormal lipid metabolism might be relevant to the changes of gut microbiota.

Experiment 1


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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

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Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon,colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Air pollution air pollution,Air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 3
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR male mice exposed to PM 2.5
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5 air
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7a & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

NCBI Quality ControlLinks
Roseburia   
Alistipes   
Lachnospiraceae   
Rikenellaceae   

Revision editor(s): WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Lwaldron, WikiWorks

Source: Figure 7a & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR male mice exposed to PM 2.5

NCBI Quality ControlLinks
Aerococcaceae   
Facklamia   
Neisseria   
Neisseriaceae   
Neisseriales   
Staphylococcaceae   
Escherichia/Shigella sp.   

Revision editor(s): Lwaldron, WikiWorks

Experiment 2


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Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 10
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

EditHistoryDelete
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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Levitest, WikiWorks, Merit, Atrayees

Source: Figure 7b & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

NCBI Quality ControlLinks
Clostridiaceae   
Proteus   
Pseudonocardiales   

Revision editor(s): Levitest, WikiWorks, Merit, Atrayees

Experiment 3


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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 21

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

EditHistoryDelete
Flag for review
Your review change was submitted
Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Merit, Atrayees

Source: Figure 7c & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

NCBI Quality ControlLinks
Bacillales   
Gemella   
Oscillibacter   
Roseburia   
Oscillibacter sp.   
Clostridium   

Revision editor(s): WikiWorks, Merit, Atrayees

Experiment 4


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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 35

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

EditHistoryDelete
Flag for review
Your review change was submitted
Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Atrayees

Source: Figure 7d& text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

NCBI Quality ControlLinks
Streptococcus   
Streptococcaceae   

Revision editor(s): WikiWorks, Atrayees

Signature 2

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2023/08/08

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Source: Figure 7d & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR male mice exposed to PM 2.5

NCBI Quality ControlLinks
Bacteroides   
Desulfovibrio   
Bacteroidaceae   
Anaerotruncus   

Revision editor(s): Atrayees

Experiment 5


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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 10
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR female mice exposed to PM 2.5

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 2

EditHistoryDelete
Flag for review
Your review change was submitted
Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Atrayees, Merit

Source: Figure 7f & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

NCBI Quality ControlLinks
Coriobacteriaceae   
Coriobacteriales   
Enterorhabdus   
Prevotellaceae   
Prevotella   

Revision editor(s): WikiWorks, Atrayees, Merit

Experiment 6


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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 21

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

EditHistoryDelete
Flag for review
Your review change was submitted
Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Rukky

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR female mice exposed to PM 2.5

NCBI Quality ControlLinks
Deltaproteobacteria   
Desulfovibrionaceae   
Desulfovibrionales   
Verrucomicrobiaceae   
Verrucomicrobiia   
Verrucomicrobiales   
Verrucomicrobiota   
Anaerostipes sp.   

Revision editor(s): WikiWorks, Rukky

Signature 2

EditHistoryDelete
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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Aiyshaaaa, WikiWorks, Merit, Atrayees

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

NCBI Quality ControlLinks
Lactobacillaceae   
Lactobacillus   
Streptophyta   

Revision editor(s): Aiyshaaaa, WikiWorks, Merit, Atrayees

Experiment 7


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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 35

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

EditHistoryDelete
Flag for review
Your review change was submitted
Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7h & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR female mice exposed to PM 2.5

NCBI Quality ControlLinks
Oscillospiraceae   

Revision editor(s): WikiWorks

Signature 2

EditHistoryDelete
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Your review change was submitted
Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Merit, Atrayees

Source: Figure 7h & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

NCBI Quality ControlLinks
Peptostreptococcaceae   
Clostridium   

Revision editor(s): WikiWorks, Merit, Atrayees

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