Sex-specific effects of PM2.5 maternal exposure on offspring's serum lipoproteins and gut microbiota

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Liu W, Zhou Y, Qin Y, Li Y, Yu L, Li R, Chen Y, Xu Y
Journal
The Science of the total environment
Year
2020
Fine particulate matter (PM2.5), which is known to impact public health, has received widespread attention recently. However, the long-term impact of maternal PM2.5 exposure remains unclear. To illuminate whether maternal PM2.5 exposure can affect serum lipoproteins and intestinal flora of offspring, mice received PM2.5 by intratracheal instillation during gestation and lactation. On postnatal day (PND) 35, serum lipoproteins of male and female pups were measured. Additionally, gut microbiota of offspring on PND 3, 10, 21 and 35 were measured by 16S rDNA sequencing of the colon contents. A higher serum triglyceride (TG) concentration in male offspring was observed in the exposed PM2.5 group (p < 0.05) compared with the control group, while there was no significant difference in lipoproteins for female offspring. On PND 35, Bacteroides, Desulfovibrio, and Anaerotruncus were enriched in the male offspring of the PM2.5-exposed group, and the control group had an increased abundance of Streptococcus. However, for female offspring on PND35, Clostridium XI was found to be enriched in the control group. A positive correlation between Bacteroides and serum TG concentration (r = 0.47, p = 0.02) was determined by Spearman's correlation analysis. These results suggest that serum TG and gut microbiota of offspring could be influenced by maternal PM2.5 exposure in a sex-specific manner. Abnormal lipid metabolism might be relevant to the changes of gut microbiota.

Experiment 1


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled (if applicable)
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
air pollution air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 3
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR male mice exposed to PM 2.5
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5 air
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7a & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

NCBI Links
Roseburia
Alistipes
Lachnospiraceae
Rikenellaceae

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Lwaldron, WikiWorks

Source: Figure 7a & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR male mice exposed to PM 2.5

NCBI Links
Aerococcaceae
Facklamia
Neisseria
Neisseriaceae
Neisseriales
Staphylococcaceae
Escherichia/Shigella sp.

Revision editor(s): Lwaldron, WikiWorks

Experiment 2


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled (if applicable)
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
air pollution air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 10
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR male mice exposed to PM 2.5
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Levitest, WikiWorks

Source: Figure 7b & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

NCBI Links
Clostridiaceae
Proteus
Pseudonocardiales
g__clostridium sensu strictog__clostridium sensu stricto

Revision editor(s): Levitest, WikiWorks

Experiment 3


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled (if applicable)
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
air pollution air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 21
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR male mice exposed to PM 2.5
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7c & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

NCBI Links
g__clostridium xivag__clostridium xiva
Bacillales Family X. Incertae Sedis
Gemella
Oscillibacter
Roseburia
Bacillales

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7c & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR male mice exposed to PM 2.5

NCBI Links
Bacteroides
Bacteroidaceae
Desulfovibrio
Anaerotruncus

Revision editor(s): WikiWorks

Experiment 4


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled (if applicable)
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
air pollution air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 35
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR male mice exposed to PM 2.5
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7d& text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

NCBI Links
Streptococcus
Streptomycetaceae

Revision editor(s): WikiWorks

Experiment 5


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled (if applicable)
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
air pollution air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 10
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR female mice exposed to PM 2.5
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7f & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR female mice exposed to PM 2.5

NCBI Links
k__bacteriak__bacteria

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Atrayees

Source: Figure 7f & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

NCBI Links
Coriobacteriales
Enterorhabdus
Prevotellaceae
g__entemoacterg__entemoacter
Coriobacteriaceae

Revision editor(s): WikiWorks, Atrayees

Experiment 6


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled (if applicable)
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
air pollution air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 21
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR female mice exposed to PM 2.5
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Subject page title: Sex-specific effects of PM2.5 maternal exposure on offspring's serum lipoproteins and gut microbiota/Experiment 6/Signature 1
Reviewer: Rukky
Review date: 2023-3-28
Review state: Reviewed
Quality control tags:





Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Rukky

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR female mice exposed to PM 2.5

NCBI Links
Deltaproteobacteria
Desulfovibrionaceae
Desulfovibrionales
Verrucomicrobiaceae
Verrucomicrobiae
Verrucomicrobiales
Verrucomicrobiota
Anaerostipes sp.

Revision editor(s): WikiWorks, Rukky

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Aiyshaaaa, WikiWorks

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

NCBI Links
Lactobacillaceae
Lactobacillus
f__chloroplastf__chloroplast
p__cyanobacteria_Chloroplastp__cyanobacteria_Chloroplast
c__chloroplastc__chloroplast
Streptophyta

Revision editor(s): Aiyshaaaa, WikiWorks

Experiment 7


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled (if applicable)
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
air pollution air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice dams exposed to filtered blanks PND 35
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICR female mice exposed to PM 2.5
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ICR mice exposed to PM 2.5
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7h & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR female mice exposed to PM 2.5

NCBI Links
Oscillospiraceae

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7h & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

NCBI Links
Peptostreptococcaceae
g__clostridium_XIg__clostridium_XI

Revision editor(s): WikiWorks