Sex-specific effects of PM2.5 maternal exposure on offspring's serum lipoproteins and gut microbiota

Study information

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

32544691

DOI Digital object identifier for electronic documents.

10.1016/j.scitotenv.2020.139982

URI

Authors

Liu W, Zhou Y, Qin Y, Li Y, Yu L, Li R, Chen Y, Xu Y

Journal

The Science of the total environment

Year

2020

Keywords:

Gut microbiota, Lipoproteins, Offspring, PM(2.5), Sex-specific

Fine particulate matter (PM2.5), which is known to impact public health, has received widespread attention recently. However, the long-term impact of maternal PM2.5 exposure remains unclear. To illuminate whether maternal PM2.5 exposure can affect serum lipoproteins and intestinal flora of offspring, mice received PM2.5 by intratracheal instillation during gestation and lactation. On postnatal day (PND) 35, serum lipoproteins of male and female pups were measured. Additionally, gut microbiota of offspring on PND 3, 10, 21 and 35 were measured by 16S rDNA sequencing of the colon contents. A higher serum triglyceride (TG) concentration in male offspring was observed in the exposed PM2.5 group (p < 0.05) compared with the control group, while there was no significant difference in lipoproteins for female offspring. On PND 35, Bacteroides, Desulfovibrio, and Anaerotruncus were enriched in the male offspring of the PM2.5-exposed group, and the control group had an increased abundance of Streptococcus. However, for female offspring on PND35, Clostridium XI was found to be enriched in the control group. A positive correlation between Bacteroides and serum TG concentration (r = 0.47, p = 0.02) was determined by Spearman's correlation analysis. These results suggest that serum TG and gut microbiota of offspring could be influenced by maternal PM2.5 exposure in a sex-specific manner. Abnormal lipid metabolism might be relevant to the changes of gut microbiota.

Experiment 1

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Curated date: 2021/01/10

Curator: WikiWorks

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Subjects

Location of subjects

China

Host species Species from which microbiome was sampled (if applicable)

Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Colon Hindgut,Large bowel,Posterior intestine,Colon

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

air pollution air pollution

Group 0 name Corresponds to the control (unexposed) group for case-control studies

mice dams exposed to filtered blanks PND 3

Group 1 name Corresponds to the case (exposed) group for case-control studies

ICR male mice exposed to PM 2.5

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

ICR mice exposed to PM 2.5 air

Group 0 sample size Number of subjects in the control (unexposed) group

8

Group 1 sample size Number of subjects in the case (exposed) group

8

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Richness Number of species

unchanged

Signature 1

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7a & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

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Roseburia
Alistipes
Lachnospiraceae
Rikenellaceae

Revision editor(s): WikiWorks

Signature 2

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Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Lwaldron, WikiWorks

Source: Figure 7a & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR male mice exposed to PM 2.5

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Aerococcaceae
Facklamia
Neisseria
Neisseriaceae
Neisseriales
Staphylococcaceae
Escherichia/Shigella sp.

Revision editor(s): Lwaldron, WikiWorks

Experiment 2

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

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Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

mice dams exposed to filtered blanks PND 10

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

ICR mice exposed to PM 2.5

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Richness Number of species

unchanged

Signature 1

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Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Levitest, WikiWorks, Merit, Atrayees

Source: Figure 7b & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

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Clostridiaceae
Proteus
Pseudonocardiales

Revision editor(s): Levitest, WikiWorks, Merit, Atrayees

Experiment 3

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

mice dams exposed to filtered blanks PND 21

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Signature 1

EditHistoryDelete

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Merit, Atrayees

Source: Figure 7c & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

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Bacillales
Gemella
Oscillibacter
Roseburia
Oscillibacter sp.
Clostridium

Revision editor(s): WikiWorks, Merit, Atrayees

Experiment 4

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

mice dams exposed to filtered blanks PND 35

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Richness Number of species

unchanged

Signature 1

EditHistoryDelete

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Atrayees

Source: Figure 7d& text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR male mice exposed to PM 2.5

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Streptococcus
Streptococcaceae

Revision editor(s): WikiWorks, Atrayees

Signature 2

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Curated date: 2023/08/08

Curator: Atrayees

Revision editor(s): Atrayees

Source: Figure 7d & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR male mice exposed to PM 2.5

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Bacteroides
Desulfovibrio
Bacteroidaceae
Anaerotruncus

Revision editor(s): Atrayees

Experiment 5

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

mice dams exposed to filtered blanks PND 10

Group 1 name Corresponds to the case (exposed) group for case-control studies

ICR female mice exposed to PM 2.5

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Signature 2

EditHistoryDelete

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Atrayees, Merit

Source: Figure 7f & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

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Coriobacteriaceae
Coriobacteriales
Enterorhabdus
Prevotellaceae
Prevotella

Revision editor(s): WikiWorks, Atrayees, Merit

Experiment 6

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

mice dams exposed to filtered blanks PND 21

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Richness Number of species

increased

Signature 1

Subject page title:	Sex-specific effects of PM2.5 maternal exposure on offspring's serum lipoproteins and gut microbiota/Experiment 6/Signature 1
Reviewer:	Rukky
Review date:	2023-3-28
Review state:	Reviewed
Quality control tags:

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Rukky

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR female mice exposed to PM 2.5

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Deltaproteobacteria
Desulfovibrionaceae
Desulfovibrionales
Verrucomicrobiaceae
Verrucomicrobiae
Verrucomicrobiales
Verrucomicrobiota
Anaerostipes sp.

Revision editor(s): WikiWorks, Rukky

Signature 2

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Aiyshaaaa, WikiWorks, Merit, Atrayees

Source: Figure 7g & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

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Lactobacillaceae
Lactobacillus
Streptophyta

Revision editor(s): Aiyshaaaa, WikiWorks, Merit, Atrayees

Experiment 7

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Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

mice dams exposed to filtered blanks PND 35

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Signature 1

EditHistoryDelete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Atrayees on 2023-8-8

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 7h & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: increased abundance in ICR female mice exposed to PM 2.5

NCBI	Quality Control	Links
Oscillospiraceae

Revision editor(s): WikiWorks

Signature 2

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Curated date: 2021/01/10

Curator: Zyaijah Bailey

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Source: Figure 7h & text

Description: LEfSe analysis of male offspring on PND 3 (a), PND 10 (b), PND 21 (c), PND 35 (d). LEfSe analysis of female offspring on PND 3 (e), PND 10 (f), PND 21 (g), PND 35 (h). N = 8/group/gender/time.

Abundance in Group 1: decreased abundance in ICR female mice exposed to PM 2.5

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Peptostreptococcaceae
Clostridium

Revision editor(s): WikiWorks, Merit, Atrayees