Effects of sex and chronic cigarette smoke exposure on the mouse cecal microbiome

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Tam A, Filho FSL, Ra SW, Yang J, Leung JM, Churg A, Wright JL, Sin DD
Journal
PloS one
Year
2020
RATIONALE: Chronic smoke exposure is associated with weight loss in patients with Chronic Obstructive Pulmonary Disease (COPD). However, the biological contribution of chronic smoking and sex on the cecal microbiome has not been previously investigated. METHODS: Adult male, female and ovariectomized mice were exposed to air (control group) or smoke for six months using a standard nose-only smoke exposure system. DNA was extracted from the cecal content using the QIAGEN QIAamp® DNA Mini Kit. Droplet digital PCR was used to generate total 16S bacterial counts, followed by Illumina MiSeq® analysis to determine microbial community composition. The sequencing data were resolved into Amplicon Sequence Variants and analyzed with the use of QIIME2®. Alpha diversity measures (Richness, Shannon Index, Evenness and Faith's Phylogenetic Diversity) and beta diversity (based on Bray-Curtis distances) were assessed and compared according to smoke exposure and sex. RESULTS: The microbial community was different between male and female mice, while ovariectomy made the cecal microbiome similar to that of male mice. Chronic smoke exposure led to significant changes in the cecal microbial community in both male and female mice. The organism, Alistipes, was the most consistent bacteria identified at the genus level in the cecal content that was reduced with chronic cigarette exposure and its expression was positively related to the whole-body weight of these mice. CONCLUSION: Chronic smoke exposure is associated with changes in the cecal content microbiome; these changes may play a role in the weight changes that are observed in cigarette smokers.

Experiment 1


Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Subjects

Location of subjects
Canada
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Caecum Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Air pollution air pollution,Air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
all other mice
Group 1 name Corresponds to the case (exposed) group for case-control studies
male mice exposed to cigarette smoke
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
male mice exposed to smoke
Group 0 sample size Number of subjects in the control (unexposed) group
50
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3.5

Alpha Diversity

Pielou Quantifies how equal the community is numerically
decreased
Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
decreased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Claregrieve1, WikiWorks, Merit

Source: Figure 7

Description: Differentially abundant taxa in the smoke-exposed male mice versus all other mice

Abundance in Group 1: increased abundance in male mice exposed to cigarette smoke

NCBI Quality ControlLinks
Akkermansia
Akkermansiaceae
Candidatus Aenigmatarchaeota
Rhodospirillales
Verrucomicrobiia
Verrucomicrobiota

Revision editor(s): Claregrieve1, WikiWorks, Merit

Experiment 2


Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
female mice exposed to cigarette smoke
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
female mice exposed to cigarette smoke
Group 0 sample size Number of subjects in the control (unexposed) group
48
Group 1 sample size Number of subjects in the case (exposed) group
10

Lab analysis

Statistical Analysis

Alpha Diversity

Pielou Quantifies how equal the community is numerically
decreased
Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure 7

Description: Differentially abundant taxa in the smoke-exposed female mice versus all other mice

Abundance in Group 1: increased abundance in female mice exposed to cigarette smoke

NCBI Quality ControlLinks
Bacteroidaceae
Bacteroides
Muribaculaceae

Revision editor(s): Claregrieve1, WikiWorks

Experiment 3


Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
ovariectomized female mice exposed to cigarette smoke
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
ovariectomized female mice exposed to smoke

Lab analysis

Statistical Analysis

Alpha Diversity

Pielou Quantifies how equal the community is numerically
decreased
Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
decreased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Claregrieve1, WikiWorks, Merit

Source: Figure 7

Description: Differentially abundant taxa in the smoke-exposed ovariectomized female mice versus all other mice

Abundance in Group 1: increased abundance in ovariectomized female mice exposed to cigarette smoke

NCBI Quality ControlLinks
Butyrivibrio
Prevotellaceae
Rikenella
Prevotella
Prevotella sp.

Revision editor(s): Claregrieve1, WikiWorks, Merit

Experiment 4


Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
control
Group 1 name Corresponds to the case (exposed) group for case-control studies
mice exposed to smoke
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
male, female or ovariectomized females mice exposed to smoke
Group 0 sample size Number of subjects in the control (unexposed) group
30
Group 1 sample size Number of subjects in the case (exposed) group
28

Lab analysis

Statistical Analysis

Alpha Diversity

Pielou Quantifies how equal the community is numerically
decreased
Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
decreased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Claregrieve1, WikiWorks, Merit

Source: Figure 2

Description: Differential microbial taxa between the control and smoke-exposed groups

Abundance in Group 1: increased abundance in mice exposed to smoke

NCBI Quality ControlLinks
Akkermansia
Akkermansia sp.
Bacteroidaceae
Bacteroides
Oscillibacter
Verrucomicrobiia
Verrucomicrobiales
Verrucomicrobiota

Revision editor(s): Claregrieve1, WikiWorks, Merit

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/7

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Claregrieve1, Merit, Atrayees

Source: Figure 2

Description: Differential microbial taxa between the control and smoke-exposed groups

Abundance in Group 1: decreased abundance in mice exposed to smoke

NCBI Quality ControlLinks
Alistipes
Lachnospiraceae
Muribaculaceae
Rikenellaceae
uncultured Bacteroidales bacterium
Lachnospira
Lachnospira sp.

Revision editor(s): WikiWorks, Claregrieve1, Merit, Atrayees