Pre-obese children's dysbiotic gut microbiome and unhealthy diets may predict the development of obesity

Study information

Needs review

study design

time series / longitudinal observational

Citation

PMID PubMed identifier for scientific articles.

30534614

DOI Digital object identifier for electronic documents.

10.1038/s42003-018-0221-5

URI

Authors

Rampelli S, Guenther K, Turroni S, Wolters M, Veidebaum T, Kourides Y, Molnár D, Lissner L, Benitez-Paez A, Sanz Y, Fraterman A, Michels N, Brigidi P, Candela M, Ahrens W

Journal

Communications biology

Year

2018

It is widely accepted that the intestinal microbiome is connected to obesity, as key mediator of the diet impact on the host metabolic and immunological status. To investigate whether the individual gut microbiome has a potential in predicting the onset and progression of diseases, here we characterized the faecal microbiota of 70 children in a two-time point prospective study, within a four-year window. All children had normal weight at the beginning of this study, but 36 of them gained excessive weight at the subsequent check-up. Microbiome data were analysed together with the hosts' diet information, physical activity, and inflammatory parameters. We find that the gut microbiota structures were stratified into a discrete number of groups, characterized by different biodiversity that correlates with inflammatory markers and dietary habits, regardless of age, gender, and body weight. Collectively, our data underscore the importance of the microbiome-host-diet configuration as a possible predictor of obesity.

Experiment 1

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Curated date: 2021/01/10

Curator: WikiWorks

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Subjects

Location of subjects: Cyprus; Estonia; Germany; Hungary; Sweden

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Group 0 name Corresponds to the control (unexposed) group for case-control studies: normal weight

Group 1 name Corresponds to the case (exposed) group for case-control studies: obese

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: children aged 2-9 years

Group 0 sample size Number of subjects in the control (unexposed) group: 34

Group 1 sample size Number of subjects in the case (exposed) group: 36

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 2 weeks

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Richness Number of species: unchanged

Signature 1

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Curated date: 2021/01/10

Curator: Mst Afroza Parvin

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Source: Table 2

Description: Microbial taxa significantly different across the four groups of children

Abundance in Group 1: increased abundance in obese

NCBI	Quality Control	Links
Bacteroidaceae
Bacteroides
Lachnospira
Roseburia

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Signature 2

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Curated date: 2021/01/10

Curator: Mst Afroza Parvin

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Source: Table 2

Description: Microbial taxa significantly different across the four groups of children

Abundance in Group 1: decreased abundance in obese

NCBI	Quality Control	Links
Prevotellaceae
Christensenellaceae
Tissierellaceae
Slackia
Prevotella
Oscillospira

Revision editor(s): WikiWorks

Experiment 2

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Curated date: 2021/01/10

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Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: before onset of obesity

Group 1 name Corresponds to the case (exposed) group for case-control studies: after onset of obesity

Group 0 sample size Number of subjects in the control (unexposed) group: 36

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 14 days