Correlation between body mass index and gut concentrations of Lactobacillus reuteri, Bifidobacterium animalis, Methanobrevibacter smithii and Escherichia coli

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Million M, Angelakis E, Maraninchi M, Henry M, Giorgi R, Valero R, Vialettes B, Raoult D
Journal
International journal of obesity (2005)
Year
2013
BACKGROUND: Genus and species level analysis is the best way to characterize alterations in the human gut microbiota that are associated with obesity, because the clustering of obese and lean microbiotas increases with the taxonomic depth of the analysis. Bifidobacterium genus members have been associated with a lean status, whereas different Lactobacillus species are associated both with a lean and an obese status. OBJECTIVES AND METHODS: We analyzed the fecal concentrations of Bacteroidetes, Firmicutes, Methanobrevibacter smithii, the genus Lactobacillus, five other Lactobacillus species previously linked with lean or obese populations, Escherichia coli and Bifidobacterium animalis in 263 individuals, including 134 obese, 38 overweight, 76 lean and 15 anorexic subjects to test for the correlation between bacterial concentration and body mass index (BMI). Of these subjects, 137 were used in our previous study. FINDINGS: Firmicutes were found in >98.5%, Bacteroidetes in 67%, M. smithii in 64%, E. coli in 51%, Lactobacillus species between 17 and 25% and B. animalis in 11% of individuals. The fecal concentration of Lactobacillus reuteri was positively correlated with BMI (coefficient=0.85; 95% confidence interval (CI) 0.12-0.58; P=0.02) in agreement with what was reported for Lactobacillus sakei. As reported, B. animalis (coefficient=-0.84; 95% CI -1.61 to -0.07; P=0.03) and M. smithii (coefficient=-0.43, 95% CI -0.90 to 0.05; P=0.08) were negatively associated with the BMI. Unexpectedly, E. coli was found here for the first time to negatively correlate with the BMI (coefficient=-1.05; 95% CI -1.60 to -0.50; P<0.001). CONCLUSION: Our findings confirm the specificity of the obese microbiota and emphasize the correlation between the concentration of certain Lactobacillus species and obesity.

Experiment 1


Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Subjects

Location of subjects
France
Host species Species from which microbiome was sampled (if applicable)
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity
Group 0 name Corresponds to the control (unexposed) group for case-control studies
anorexic, lean, and overweight subjects
Group 1 name Corresponds to the case (exposed) group for case-control studies
obese subjects
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
patients who were obese by BMI (BMI>30 kg m−2)
Group 0 sample size Number of subjects in the control (unexposed) group
128
Group 1 sample size Number of subjects in the case (exposed) group
134
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months

Lab analysis

Sequencing type
WMS
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
RT-qPCR

Statistical Analysis

Statistical test
Chi-Square
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplementary Table 1

Description: Differential microbial abundance between the obese group and all the other groups

Abundance in Group 1: decreased abundance in obese subjects

NCBI Quality ControlLinks
Bacteroidota

Revision editor(s): Claregrieve1, WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
lean and anorexic subjects
Group 1 name Corresponds to the case (exposed) group for case-control studies
obese and overweight subjects
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
patients who were overweight or obese by BMI
Group 0 sample size Number of subjects in the control (unexposed) group
91
Group 1 sample size Number of subjects in the case (exposed) group
171

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplementary Table 1

Description: Differential microbial abundance between the obese and overweight group and the lean and anorexic group

Abundance in Group 1: decreased abundance in obese and overweight subjects

NCBI Quality ControlLinks
Methanobrevibacter smithii

Revision editor(s): Claregrieve1, WikiWorks

Experiment 3


Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
lean subjects
Group 1 name Corresponds to the case (exposed) group for case-control studies
obese subjects
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
obese patients defined by BMI (BMI>30 kg m−2)
Group 0 sample size Number of subjects in the control (unexposed) group
64
Group 1 sample size Number of subjects in the case (exposed) group
108

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/1

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplementary Table 1

Description: Differential microbial abundance between obese and lean subjects

Abundance in Group 1: decreased abundance in obese subjects

NCBI Quality ControlLinks
Escherichia coli

Revision editor(s): Claregrieve1, WikiWorks

Signature 2

Needs review

Curated date: 2022/08/09

Curator: Claregrieve1

Revision editor(s): Claregrieve1

Source: Supplementary Table 1

Description: Differential microbial abundance between obese and lean subjects

Abundance in Group 1: increased abundance in obese subjects

NCBI Quality ControlLinks
Limosilactobacillus reuteri

Revision editor(s): Claregrieve1

Experiment 4


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
overweight subjects
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
patients who were overweight by BMI (BMI>25 and <30 kg m−2)
Group 1 sample size Number of subjects in the case (exposed) group
32

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2021/01/10

Curator: Mst Afroza Parvin

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplementary Table 1

Description: Differential microbial abundance between overweight and lean subjects

Abundance in Group 1: increased abundance in overweight subjects

NCBI Quality ControlLinks
Limosilactobacillus reuteri

Revision editor(s): Claregrieve1, WikiWorks

Experiment 5


Needs review

Curated date: 2022/08/09

Curator: Claregrieve1

Revision editor(s): Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
anorexic, lean, and overweight subjects
Group 1 name Corresponds to the case (exposed) group for case-control studies
obese subjects
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
patients who were obese by BMI (BMI>30 kg m−2)
Group 0 sample size Number of subjects in the control (unexposed) group
111
Group 1 sample size Number of subjects in the case (exposed) group
108

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2022/08/09

Curator: Claregrieve1

Revision editor(s): Claregrieve1

Source: Supplementary Table 1

Description: Differential microbial abundance between the obese group and all the other groups

Abundance in Group 1: decreased abundance in obese subjects

NCBI Quality ControlLinks
Escherichia coli
Bifidobacterium animalis

Revision editor(s): Claregrieve1