Probiotics Ameliorate Colon Epithelial Injury Induced by Ambient Ultrafine Particles Exposure

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Li X, Sun H, Li B, Zhang X, Cui J, Yun J, Yang Y, Zhang L, Meng Q, Wu S, Duan J, Yang H, Wu J, Sun Z, Zou Y, Chen R
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Year
2019
Keywords:
Lactobacillus, air pollution, colonic epithelium, gut microbiota, ultrafine particles
Diesel exhaust particles (DEPs) are common airborne ultrafine particles (UFPs); however, few studies have examined their effects on the gastrointestinal tract. To investigate the interaction of gut microbiota and DEPs-induced colonic injury, adult C57BL/6 mice are kept in whole-body inhalation chambers and exposed to filtered room air (FRA) or DEPs (300 µg m-3) 1 h per day for 28 consecutive days. DEPs exposure results in colon epithelial injury with inflammatory cell infiltration and mucus depletion. Abundance of Lactobacillus in murine feces is transiently increased following 7-day DEPs exposure and then decreased until the end of 28-day exposure. A reduction of the colonic mucus layer thickness is observed in mice receiving gut microbiota from DEPs-exposed mice. Mechanistically, RNA-sequencing suggests disruption of the nitrogen metabolism pathway in DEPs-exposed NCM460 cells. Upregulation of carbonic anhydrase 9 (CA9) expression levels is observed in epithelia following DEPs exposure both in vivo and in vitro. Oral administration of probiotics protects the mice against DEPS-induced colon epithelial injury. The results strongly suggest the involvement of gut microbiota in response to DEPs exposure and subsequently epithelial injury in vivo. Supplementation with probiotic may be a potential way to protect against UFPs-induced colon epithelial injury.

Experiment 1


Needs review

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon,colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Air pollution air pollution,Air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
mice exposed to FRA
Group 1 name Corresponds to the case (exposed) group for case-control studies
Diesel exhaust particle (DEP) exposed mice
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
mice exposed to Diesel exhaust particles (DEP)
Group 0 sample size Number of subjects in the control (unexposed) group
30
Group 1 sample size Number of subjects in the case (exposed) group
37

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4


Signature 1

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks

Source: Figure 2C & text

Description: LEfSe histogram of fecal microbiota profiling from DEPs group (fecal samples from male n = 20 and female n = 17) and FRA group (fecal samples from male n = 15 and female n = 15) following 7‐day exposure.

Abundance in Group 1: increased abundance in Diesel exhaust particle (DEP) exposed mice

NCBI Quality ControlLinks
Bacilli
Lactobacillales
Lactobacillus

Revision editor(s): WikiWorks

Signature 2

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Aiyshaaaa, WikiWorks, Merit

Source: Figure 2C & text

Description: LEfSe histogram of fecal microbiota profiling from DEPs group (fecal samples from male n = 20 and female n = 17) and FRA group (fecal samples from male n = 15 and female n = 15) following 7‐day exposure.

Abundance in Group 1: decreased abundance in Diesel exhaust particle (DEP) exposed mice

NCBI Quality ControlLinks
Bacillota
Bacteroidaceae
Bacteroides
Clostridia
Eubacteriales
Lachnospiraceae
Clostridiaceae
Clostridiales bacterium
Clostridium
Clostridium sp.
Bacteroidales

Revision editor(s): Aiyshaaaa, WikiWorks, Merit