Probiotics Ameliorate Colon Epithelial Injury Induced by Ambient Ultrafine Particles Exposure

Study information

Needs review

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

31559135

DOI Digital object identifier for electronic documents.

10.1002/advs.201900972

URI

Authors

Li X, Sun H, Li B, Zhang X, Cui J, Yun J, Yang Y, Zhang L, Meng Q, Wu S, Duan J, Yang H, Wu J, Sun Z, Zou Y, Chen R

Journal

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Year

2019

Keywords:

Lactobacillus, air pollution, colonic epithelium, gut microbiota, ultrafine particles

Diesel exhaust particles (DEPs) are common airborne ultrafine particles (UFPs); however, few studies have examined their effects on the gastrointestinal tract. To investigate the interaction of gut microbiota and DEPs-induced colonic injury, adult C57BL/6 mice are kept in whole-body inhalation chambers and exposed to filtered room air (FRA) or DEPs (300 µg m-3) 1 h per day for 28 consecutive days. DEPs exposure results in colon epithelial injury with inflammatory cell infiltration and mucus depletion. Abundance of Lactobacillus in murine feces is transiently increased following 7-day DEPs exposure and then decreased until the end of 28-day exposure. A reduction of the colonic mucus layer thickness is observed in mice receiving gut microbiota from DEPs-exposed mice. Mechanistically, RNA-sequencing suggests disruption of the nitrogen metabolism pathway in DEPs-exposed NCM460 cells. Upregulation of carbonic anhydrase 9 (CA9) expression levels is observed in epithelia following DEPs exposure both in vivo and in vitro. Oral administration of probiotics protects the mice against DEPS-induced colon epithelial injury. The results strongly suggest the involvement of gut microbiota in response to DEPs exposure and subsequently epithelial injury in vivo. Supplementation with probiotic may be a potential way to protect against UFPs-induced colon epithelial injury.

Experiment 1

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Curated date: 2021/01/10

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Subjects

Location of subjects: China

Host species Species from which microbiome was sampled (if applicable): Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Colon Hindgut,Large bowel,Posterior intestine,Colon

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: air pollution air pollution

Group 0 name Corresponds to the control (unexposed) group for case-control studies: mice exposed to FRA
Group 1 name Corresponds to the case (exposed) group for case-control studies: Diesel exhaust particle (DEP) exposed mice
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: mice exposed to Diesel exhaust particles (DEP)
Group 0 sample size Number of subjects in the control (unexposed) group: 30
Group 1 sample size Number of subjects in the case (exposed) group: 37

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 4

Signature 1

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Curated date: 2021/01/10

Curator: Zyaijah Bailey

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Source: Figure 2C & text

Description: LEfSe histogram of fecal microbiota profiling from DEPs group (fecal samples from male n = 20 and female n = 17) and FRA group (fecal samples from male n = 15 and female n = 15) following 7‐day exposure.

Abundance in Group 1: increased abundance in Diesel exhaust particle (DEP) exposed mice

NCBI	Quality Control	Links
Bacilli
Lactobacillales
Lactobacillus

Revision editor(s): WikiWorks

Signature 2

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Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): Aiyshaaaa, WikiWorks, Merit

Source: Figure 2C & text

Description: LEfSe histogram of fecal microbiota profiling from DEPs group (fecal samples from male n = 20 and female n = 17) and FRA group (fecal samples from male n = 15 and female n = 15) following 7‐day exposure.

Abundance in Group 1: decreased abundance in Diesel exhaust particle (DEP) exposed mice

NCBI	Quality Control	Links
Bacillota
Bacteroidaceae
Bacteroides
Clostridia
Eubacteriales
Lachnospiraceae
Clostridiaceae
Clostridiales bacterium
Clostridium
Clostridium sp.
Bacteroidales

Revision editor(s): Aiyshaaaa, WikiWorks, Merit