Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection

From BugSigDB
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/11
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Pushalkar S, Paul B, Li Q, Yang J, Vasconcelos R, Makwana S, González JM, Shah S, Xie C, Janal MN, Queiroz E, Bederoff M, Leinwand J, Solarewicz J, Xu F, Aboseria E, Guo Y, Aguallo D, Gomez C, Kamer A, Shelley D, Aphinyanaphongs Y, Barber C, Gordon T, Corby P, Li X, Saxena D
Journal
iScience
Year
2020
Keywords:
In Vitro Toxicology, Microbiome, Oral Microbiology
The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human participants, 40 in each of the three cohorts, and found significantly altered beta-diversity in e-cigarette users (p = 0.006) when compared with never smokers or tobacco cigarette smokers. The abundance of Porphyromonas and Veillonella (p = 0.008) was higher among vapers. Interleukin (IL)-6 and IL-1β were highly elevated in e-cigarette users when compared with non-users. Epithelial cell-exposed e-cigarette aerosols were more susceptible for infection. In vitro infection model of premalignant Leuk-1 and malignant cell lines exposed to e-cigarette aerosol and challenged by Porphyromonas gingivalis and Fusobacterium nucleatum resulted in elevated inflammatory response. Our findings for the first time demonstrate that e-cigarette users are more prone to infection.

Experiment 1


Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/11

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Smoking behavior smoking,Smoking behavior,smoking behavior
Group 0 name Corresponds to the control (unexposed) group for case-control studies
non-smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
e-cigarette smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
participants who were 21 years or older, healthy, diagnosed with periodontal disease, and current e-cigarette smokers
Group 0 sample size Number of subjects in the control (unexposed) group
39
Group 1 sample size Number of subjects in the case (exposed) group
40
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/11

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure S2

Description: Differential microbial abundance between e-cig smokers and never smokers

Abundance in Group 1: decreased abundance in e-cigarette smokers

NCBI Quality ControlLinks
Fusobacterium periodonticum
Fusobacterium sp.
Lachnoanaerobaculum orale
Lautropia mirabilis
Oribacterium parvum
Peptococcus sp.
Prevotella sp.
Streptococcus oralis
Porphyromonas pasteri

Revision editor(s): Claregrieve1, WikiWorks

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/11

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure S2

Description: Differential microbial abundance between e-cig smokers and never smokers

Abundance in Group 1: increased abundance in e-cigarette smokers

NCBI Quality ControlLinks
Alloscardovia omnicolens
Eubacteriales
Filifactor alocis
Fretibacterium fastidiosum
Fusobacterium nucleatum
Lactococcus lactis
Mitsuokella sp.
Neisseria bacilliformis
Oribacterium sp.
Parvimonas micra
Porphyromonas endodontalis
Porphyromonas uenonis
Hallella multisaccharivorax
Segatella oris
Prevotella sp.
Selenomonas artemidis
Selenomonas dianae
Slackia exigua
Staphylococcus capitis
Veillonella dispar
Veillonellaceae
uncultured Bacteroidota bacterium

Revision editor(s): Claregrieve1, WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/11

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
never smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
cigarette smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
participants who were 21 years or older, healthy, diagnosed with periodontal disease, and current smokers

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure S2

Description: Differential microbial abundance between cigarette smokers and never smokers

Abundance in Group 1: increased abundance in cigarette smokers

NCBI Quality ControlLinks
Abiotrophia defectiva
Actinomyces gerencseriae
Capnocytophaga gingivalis
Capnocytophaga haemolytica
Capnocytophaga leadbetteri
Capnocytophaga sputigena
Cardiobacterium hominis
Catonella morbi
Corynebacterium durum
Eikenella corrodens
Fusobacterium periodonticum
Granulicatella elegans
Haemophilus haemolyticus
Haemophilus parahaemolyticus
Haemophilus sp.
Lachnoanaerobaculum orale
Lautropia mirabilis
Neisseria oralis
Neisseria sp.
Oribacterium parvum
Oscillospiraceae
Ottowia sp.
Peptococcus sp.
Peptostreptococcus stomatis
Porphyromonas pasteri
Prevotella sp.
Rothia mucilaginosa
Solobacterium moorei
Streptococcus oralis
Veillonella rogosae

Revision editor(s): Claregrieve1, WikiWorks

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): Lwaldron, Claregrieve1, WikiWorks

Source: Figure S2

Description: Differential microbial abundance between cigarette smokers and never smokers

Abundance in Group 1: decreased abundance in cigarette smokers

NCBI Quality ControlLinks
Actinomyces lingnae
Alloscardovia omnicolens
Desulfobulbus sp.
Eubacteriales
Filifactor alocis
Fretibacterium fastidiosum
Fusobacterium nucleatum
Lactobacillus gasseri
Lactococcus lactis
Megasphaera micronuciformis
Megasphaera sp.
Mitsuokella sp.
Mollicutes
Oribacterium sp.
Parvimonas micra
Peptoniphilus lacrimalis
Porphyromonas endodontalis
Porphyromonas uenonis
Prevotella denticola
Prevotella intermedia
Segatella maculosa
Hallella multisaccharivorax
Hoylesella oralis
Segatella oris
Segatella oulorum
Prevotella sp.
Prevotella veroralis
Pyramidobacter piscolens
Scardovia wiggsiae
Selenomonas artemidis
Selenomonas dianae
Slackia exigua
Streptococcus parasanguinis
Treponema denticola
Treponema maltophilum
Treponema sp.
Veillonella atypica
Veillonella dispar
Veillonella sp.
Veillonellaceae

Revision editor(s): Lwaldron, Claregrieve1, WikiWorks

Experiment 3


Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
cigarette smoker
Group 1 name Corresponds to the case (exposed) group for case-control studies
E-cigarette smoker
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
participants who were 21 years or older, healthy, diagnosed with periodontal disease, and current e-cigarette users
Group 0 sample size Number of subjects in the control (unexposed) group
40

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure S2

Description: Differential microbial abundance between cigarette smokers and e-cigarette smokers

Abundance in Group 1: increased abundance in E-cigarette smoker

NCBI Quality ControlLinks
Capnocytophaga gingivalis
Capnocytophaga haemolytica
Capnocytophaga sputigena
Cardiobacterium hominis
Catonella morbi
Corynebacterium durum
Eikenella corrodens
Fusobacterium periodonticum
Haemophilus haemolyticus
Haemophilus sp.
Lautropia mirabilis
Neisseria oralis
Neisseria sp.
Oscillospiraceae
Peptococcus sp.
Peptostreptococcus stomatis
Porphyromonas pasteri
Prevotella sp.
Rothia mucilaginosa
Veillonella rogosae

Revision editor(s): Claregrieve1, WikiWorks

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/13

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): Claregrieve1, WikiWorks

Source: Figure S2

Description: Differential microbial abundance between cigarette smokers and e-cigarette smokers

Abundance in Group 1: decreased abundance in E-cigarette smoker

NCBI Quality ControlLinks
Actinomyces lingnae
Alloscardovia omnicolens
Desulfobotulus sp.
Megasphaera micronuciformis
Prevotella sp.
Streptococcus parasanguinis

Revision editor(s): Claregrieve1, WikiWorks