HPV/Chlamydia trachomatis co-infection: metagenomic analysis of cervical microbiota in asymptomatic women

From BugSigDB
Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Di Pietro M, Filardo S, Porpora MG, Recine N, Latino MA, Sessa R
Journal
The new microbiologica
Year
2018
Keywords:
Cervical microbiota, Chlamydia trachomatis, Coinfection, HPV, Metagenomic analysis
HPV and Chlamydia trachomatis are the most common causes of sexually transmitted diseases worldwide. Most infections are asymptomatic and left untreated lead to severe reproductive tract sequelae such as cervical cancer and infertility. Interestingly, C. trachomatis may also increase the susceptibility to HPV infection as well as contribute to viral persistence. Recently, a growing body of evidence has suggested that the composition of the cervico-vaginal microbiota plays a key role in the susceptibility and outcome of genital infections caused by several pathogens, including HPV and C. trachomatis. The aim of our study was to undertake a metagenomic analysis of sequenced 16s rRNA gene amplicons to characterize the cervical microbiota from asymptomatic women with HPV/C. trachomatis co-infection. The composition of the cervical microbiota from HPV-positive or C. trachomatis-positive women was also analysed. The main finding of our study showed that the cervical microbiota in HPV/C. trachomatis co-infected women had a higher microbial diversity than the cervical microbiota in healthy controls (p<0.05). In addition, Aerococcus christensenii was associated with C. trachomatis infection. In conclusion, the increased cervical microbial diversity observed in HPV/C. trachomatis co-infected women and the detection of potential microbiological biomarkers of C. trachomatis infection will open the way to innovative approaches that may be helpful to identify women at risk of co-infection.

Experiment 1


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects
Italy
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Endocervix Endocervix,endocervix
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Chlamydia trachomatis infectious disease chlamydia,Chlamydia Infections,Chlamydia trachomatis caused disease or disorder,Chlamydia trachomatis disease or disorder,Chlamydia trachomatis infectious disease,chlamydia trachomatis infectious disease,chlamydial disease,Chlamydial Infection,Chlamydial infection
Group 0 name Corresponds to the control (unexposed) group for case-control studies
healthy control
Group 1 name Corresponds to the case (exposed) group for case-control studies
patient with chlamydia infection
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
patient with Chlamydia infection
Group 0 sample size Number of subjects in the control (unexposed) group
7
Group 1 sample size Number of subjects in the case (exposed) group
7
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Current

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks

Source: Figure 3

Description: LEfSe results on cervical microbiota in C. trachomatis-positive women compared to HPV-positive women or healthy controls.

Abundance in Group 1: increased abundance in patient with chlamydia infection

NCBI Quality ControlLinks
Aerococcus
Aerococcaceae
Aerococcus christensenii

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks

Source: Figure 3

Description: LEfSe results on cervical microbiota in C. trachomatis-positive women compared to HPV-positive women or healthy controls.

Abundance in Group 1: decreased abundance in patient with chlamydia infection

NCBI Quality ControlLinks
Lactobacillales
Lactobacillus
Lactobacillaceae
Bacillota
Bacilli

Revision editor(s): WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Human papilloma virus infection , Chlamydophila infectious disease HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease,Human papilloma virus infection,Chlamydia caused disease or disorder,Chlamydia disease or disorder,Chlamydia infection,Chlamydia infectious disease,chlamydia infectious disease,Chlamydophila caused disease or disorder,Chlamydophila disease or disorder,Chlamydophila Infections,infection, Chlamydia,infections, Chlamydia,Chlamydophila infectious disease,chlamydophila infectious disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
HPV positive women
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks

Source: Figure 3

Description: LEfSe results on cervical microbiota in C. trachomatis-positive women compared to HPV-positive women or healthy controls.

Abundance in Group 1: increased abundance in patient with chlamydia infection

NCBI Quality ControlLinks
Chlamydiota
Chlamydiaceae
Chlamydiia
Chlamydiales
Chlamydia
Chlamydia trachomatis

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Fatima Zohra on 2021/02/09

Curated date: 2021/01/10

Curator: Cynthia Anderson

Revision editor(s): WikiWorks

Source: Figure 3

Description: LEfSe results on cervical microbiota in C. trachomatis-positive women compared to HPV-positive women or healthy controls.

Abundance in Group 1: decreased abundance in patient with chlamydia infection

NCBI Quality ControlLinks
Lactobacillaceae
Lactobacillus
Bacilli
Lactobacillales
Bacillota

Revision editor(s): WikiWorks