Dysbiosis of gut microbiota in patients with neuromyelitis optica spectrum disorders: A cross sectional study

Study information

Reviewed Marked as Reviewed by Atrayees on 2023-6-29

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

31841737

DOI Digital object identifier for electronic documents.

10.1016/j.jneuroim.2019.577126

URI

Authors

Shi Z, Qiu Y, Wang J, Fang Y, Zhang Y, Chen H, Du Q, Zhao Z, Yan C, Yang M, Zhou H

Journal

Journal of neuroimmunology

Year

2020

BACKGROUND: Accumulating evidence points to an association of alternations in the gut microbiota with health and disease, including the development of neurological diseases. However, there are relatively scarce studies of the role of the gut microbiota in neuromyelitis optica spectrum disorders (NMOSD). Therefore, the aim of the present study was to evaluate the differences in the intestinal microbiota composition between patients with NMOSD and healthy control subjects. METHODS: This was a cross-sectional study. Stool samples were obtained from 20 patients with NMOSD and 20 healthy family members of the patients as controls (HC). The bacterial 16S rRNA gene amplification sequencing (V3-V4 region) was used to detect the composition and structure of the intestinal microbiota community in the two groups. RESULTS: The gut microbiota compositions clearly differed between the NMOSD and HC groups, although there was no significant difference in the overall microbial community structure. In detail, patients with NMOSD had an increased abundance of the pathogenic genera Flavonifractor (P = .004) and Streptococcus (P = .007) compared with the HC. In addition, several intestinal commensal bacteria were detected at significantly lower abundance in the NMOSD patients compared to the controls, including Faecalibacterium, Lachnospiracea_incertae_sedis, Prevotella, Blautia, Roseburia, Romboutsia, Coprococcus, and Fusicatenibacter (all P < .05). ROC curve analysis suggested that gut microbiota genera had potential to distinguish NMOSD from controls. Functional analysis further indicated that the gut microbiome of NMOSD patients was associated with three significantly downregulated metabolic pathways: "Photosynthesis" (P < .001), "Photosynthesis proteins" (P < .001), and "Thiamine metabolism" (P = .007). These differences remained significant even after correction for multiple comparisons (all PFDR < 0.05). CONCLUSION: Our results reveal the dysbiosis of intestinal bacteria and regarding metabolic abnormalities in patients with NMOSD. Further studies are warranted to elucidate the potential mechanism by which dysbiosis of microbiota contributes to the onset and progression of NMOSD.

Experiment 1

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Reviewed Marked as Reviewed by Atrayees on 2023-6-29

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: neuromyelitis optica Devic disease,Devic syndrome,Devic's disease,Devic's neuromyelitis optica,Devic's syndrome,neuromyelitis optica,NMO

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls
Group 1 name Corresponds to the case (exposed) group for case-control studies: neuromyelitis optica spectrum disorder
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Each patient met the 2015 international diagnostic criteria for NMOSD and tested positive for serum AQP4-IgG using cell-based assays
Group 0 sample size Number of subjects in the control (unexposed) group: 20
Group 1 sample size Number of subjects in the case (exposed) group: 20
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): currently taking antibiotics

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 3

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged
Chao1 Abundance-based estimator of species richness: unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged
Richness Number of species: unchanged

Signature 1

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Reviewed Marked as Reviewed by Atrayees on 2023-6-29

Curated date: 2021/01/10

Curator: Rimsha Azhar

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Source: Figure 3

Description: Abundance of gut microbiota between the NMOSD and HS groups

Abundance in Group 1: increased abundance in neuromyelitis optica spectrum disorder

NCBI	Quality Control	Links
Enterobacterales
Enterobacteriaceae
Pseudomonadota
Gammaproteobacteria
Escherichia
Shigella
Bacilli
Bacillales
Lactobacillales
Oribacterium
Anaerobacterium
Anaeroglobus
Flavonifractor
Streptococcus
Streptococcaceae

Revision editor(s): WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Atrayees on 2023-6-29

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks, Atrayees

Source: Figure 3, text

Description: Abundance of gut microbiota between the NMOSD and HS groups

Abundance in Group 1: decreased abundance in neuromyelitis optica spectrum disorder

NCBI	Quality Control	Links
Sutterella
Fusicatenibacter
Coprococcus
Peptostreptococcaceae
Romboutsia
Blautia
Roseburia
Lachnospiraceae
Faecalibacterium
Prevotellaceae
Eubacteriales
Clostridia
Prevotella

Revision editor(s): WikiWorks, Atrayees