Alterations in the skin microbiome are associated with disease severity and treatment in the perioral zone of the skin of infants with atopic dermatitis

Study information

Reviewed Marked as Reviewed by Folakunmi on 2024-1-11

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

31152265

DOI Digital object identifier for electronic documents.

10.1007/s10096-019-03598-9

URI

Authors

Zheng Y, Wang Q, Ma L, Chen Y, Gao Y, Zhang G, Cui S, Liang H, He C, Song L

Journal

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

Year

2019

Keywords:

Atopic dermatitis, Bacterial diversity, Perioral skin, Prevotella melaninogenica

Atopic dermatitis (AD), a chronic relapsing inflammatory pruritic skin disorder with a unique pathophysiology, has a high incidence in the perioral zone among infants. This study aimed to analyze the association of skin microfloral dynamics with disease severity and treatment of AD in 0-1-year-old infants. Based on the eczema area and severity index, subjects were divided into five groups, i.e., mild, moderate, severe, and severe post-treatment, with a healthy control group, and bacterial density at the perioral lesion, disease severity, and treatment were assessed in 0-1-year-old infants with AD. The perioral lesions were colonized predominantly by Firmicutes, followed in abundance by Proteobacteria, Actinobacteria, and Bacteroidetes. In the phylum Firmicutes, Streptococcus was the most predominant genus. In AD infants, the abundance of Bacteroidetes and Fusobacterium decreased significantly with an increase in disease severity (p < 0.01). The abundance of 6 genera, including Prevotella, decreased significantly with an increase in disease severity (p < 0.05). The abundance of Prevotella melaninogenica decreased gradually with an increase in disease severity and increased after treatment; this trend was reversed for Corynebacterium simulans. A reduction in the abundance of Staphylococcus and an increase in that of skin microflora including Prevotella spp., Staphylococcus epidermidis, and Erwinia dispersa were associated with treatment and clinical improvement. Skin bacterial composition varies with AD severity, and Corynebacterium simulans and Prevotella melaninogenica are positively and negatively correlated with AD severity, respectively. This study provides a theoretical basis to identify potential biomarkers AD occurrence and pathogenesis.

Experiment 1

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Reviewed Marked as Reviewed by Folakunmi on 2024-1-11

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Skin of body Entire integument,Entire skin,Integument,Integumental organ,Pelt,Skin,Skin organ,Skin of body,skin of body

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Atopic eczema allergic,allergic dermatitis,allergic form of dermatitis,Atopic Dermatitides,Atopic Dermatitis,Atopic dermatitis,atopic dermatitis and related conditions,Atopic dermatitis and related conditions (disorder),atopic eczema,Atopic Neurodermatitides,Atopic Neurodermatitis,Atopic neurodermatitis,Besnier's prurigo,Dermatitides, Atopic,Dermatitis, Atopic,Disseminated Neurodermatitides,Disseminated Neurodermatitis,eczema,Eczema, Atopic,Eczema, Infantile,eczematous dermatitis,Infantile Eczema,Neurodermatitides, Atopic,Neurodermatitides, Disseminated,Neurodermatitis, Atopic,Neurodermatitis, Disseminated,OTHER ATOPIC DERMATITIS,Other atopic dermatitis and related conditions,Atopic eczema

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy control

Group 1 name Corresponds to the case (exposed) group for case-control studies: severe atopic dermatitis

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: child with severe atopic dermatitis based on eczema area and severity index (EASI) local rating method

Group 0 sample size Number of subjects in the control (unexposed) group: 20

Group 1 sample size Number of subjects in the case (exposed) group: 20

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V2

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Chao1 Abundance-based estimator of species richness: decreased

Simpson Estimator of species richness and species evenness: more weight on species evenness: increased

Richness Number of species: decreased

Signature 1

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Reviewed Marked as Reviewed by Folakunmi on 2024-1-11

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Table 2, Table 3

Description: Different genera of average relative abundance >=0.1% in healthy and severe atopic dermatitis groups

Abundance in Group 1: increased abundance in severe atopic dermatitis

NCBI	Quality Control	Links
Staphylococcus
Rhodococcus
Alloiococcus
Tepidimonas
Staphylococcus aureus
Corynebacterium simulans
Sphingomonas yabuuchiae

Revision editor(s): WikiWorks

Signature 2

Edit History Delete

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Reviewed Marked as Reviewed by Folakunmi on 2024-1-11

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks, Folakunmi

Source: Table 2, Table 3

Description: Different genera of average relative abundance >=0.1% in healthy and severe atopic dermatitis groups

Abundance in Group 1: decreased abundance in severe atopic dermatitis

NCBI	Quality Control	Links
Agrobacterium
Atopobium
Hoylesella nanceiensis
Leptotrichia
Limnobacter
Porphyromonas
Prevotella
Prevotella melaninogenica
Rothia
Rothia mucilaginosa
Streptococcus
Veillonella
Granulicatella

Revision editor(s): WikiWorks, Folakunmi