Variations in early gut microbiome are associated with childhood eczema

From BugSigDB
Reviewed Marked as Reviewed by Lwaldron on 2022/03/30
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Zhang Y, Jin S, Wang J, Zhang L, Mu Y, Huang K, Zhao B, Zhang K, Cui Y, Li S
Journal
FEMS microbiology letters
Year
2019
Keywords:
Bifidobacterium, 16S rRNA gene sequencing, childhood eczema, diversity, gut microbiome, qPCR
We assessed the relationship between gut microbiome profile and childhood eczema in 172 subjects (age < 3 years, healthy group N = 123, eczema group N = 49) utilizing 16S rRNA gene sequencing. Lower relative abundance of Bifidobacterium was shown to be associated with childhood eczema. Considering that developmental and environmental factors could modify the state of children's gut microbiome, we divided the samples into four age groups: 0-0.5 years, 0.5-1 years, 1-2 years and 2-3 years for farther analyses. Data revealed significant inter-group differences between healthy and eczema samples in all age groups, and decreased microbial diversity was most significantly found in children with eczema of age 2-3 years old. Decreased abundance of Bifidobacterium was a major finding in eczema groups from 0.5-3 years compared to the age matched healthy controls, but not significant in children younger than 6 month old. Of note, Bifidobacterium operational taxonomic units were identified by Random Forest with highly predictive power of 0.83 (AUC = 0.83) in ROC analysis, which also confirmed its role as a key genus that is associated with eczema. To verify the sequencing results, we performed quantitative polymerase chain reaction of Bifidobacterium and Bacteroides in the same cohort, and in a new eczema cohort (N = 57) for validation. Significantly, lower Bifidobacterium quantities were found in both eczema groups with an age range of 0.5-3 years. These results suggest variations in early gut microbiome are associated with childhood eczema.

Experiment 1


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks, Victoria

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Eczema Eczema,eczema
Group 0 name Corresponds to the control (unexposed) group for case-control studies
healthy control
Group 1 name Corresponds to the case (exposed) group for case-control studies
child with eczema
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
children age 0-3 with eczema diagnosed by pediatrician based on clinical criteria such as dry, desquamation skin, increased temperature on the local skin, and oozing or crusting
Group 0 sample size Number of subjects in the control (unexposed) group
123
Group 1 sample size Number of subjects in the case (exposed) group
49

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
Matched on Factors on which subjects have been matched on in a case-control study
age

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3a

Description: Significantly diverse bacterial communities between healthy and eczema groups in age groups 0-3 years

Abundance in Group 1: increased abundance in child with eczema

NCBI Quality ControlLinks
Roseburia
Erwinia

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3a

Description: Significantly diverse bacterial communities between healthy and eczema groups in age groups 0-3 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Bifidobacterium
Enterobacter

Revision editor(s): WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): Lwaldron, WikiWorks

Source: Figure 3b

Description: Significantly diverse bacterial communities between healthy and eczema groups in age groups 0-3 years

Abundance in Group 1: increased abundance in child with eczema

NCBI Quality ControlLinks
Clostridia
Eubacteriales
Veillonella parvula
Clostridium neonatale

Revision editor(s): Lwaldron, WikiWorks

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3b

Description: Significantly diverse bacterial communities between healthy and eczema groups in age groups 0-3 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Actinomycetota
Actinomycetes
Bifidobacterium
Bifidobacteriales
Bifidobacteriaceae
Bifidobacterium longum subsp. longum
Pseudomonadota

Revision editor(s): WikiWorks

Experiment 3


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
children age 0-0.5 with eczema diagnosed by pediatrician based on clinical criteria such as dry, desquamation skin, increased temperature on the local skin, and oozing or crusting

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
T-Test
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3c

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 0-0.5 years

Abundance in Group 1: increased abundance in child with eczema

NCBI Quality ControlLinks
Erwinia

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3c

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 0-0.5 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Faecalibacterium
Lachnospira

Revision editor(s): WikiWorks

Experiment 4


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3d

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 0-0.5 years

Abundance in Group 1: increased abundance in child with eczema

NCBI Quality ControlLinks
Veillonella parvula
Bacteroides fragilis

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3d

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 0-0.5 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Bifidobacterium bifidum

Revision editor(s): WikiWorks

Experiment 5


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
children age 0.5-1 with eczema diagnosed by pediatrician based on clinical criteria such as dry, desquamation skin, increased temperature on the local skin, and oozing or crusting

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
T-Test
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3e

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 0.5-1 years

Abundance in Group 1: increased abundance in child with eczema

NCBI Quality ControlLinks
Erwinia

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3e

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 0.5-1 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Bifidobacterium
Streptococcus
Campylobacter

Revision editor(s): WikiWorks

Experiment 6


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3f

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 0.5-1 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Faecalibacterium
Faecalibacterium prausnitzii
Actinomycetota
Actinomycetes
Bifidobacterium
Bifidobacteriales
Bifidobacteriaceae
Bifidobacterium longum subsp. longum
Enterobacter

Revision editor(s): WikiWorks

Experiment 7


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
children age 1-2 with eczema diagnosed by pediatrician based on clinical criteria such as dry, desquamation skin, increased temperature on the local skin, and oozing or crusting

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
T-Test
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks, Merit

Source: Figure 3g

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 1-2 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Bifidobacterium
Enterobacter
Klebsiella
Streptococcus
Veillonella

Revision editor(s): WikiWorks, Merit

Experiment 8


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3h

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 1-2 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Actinomycetota

Revision editor(s): WikiWorks

Experiment 9


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
children age 2-3 with eczema diagnosed by pediatrician based on clinical criteria such as dry, desquamation skin, increased temperature on the local skin, and oozing or crusting

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
T-Test
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3i

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 2-3 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Bifidobacterium
Sutterella
Clostridium
Collinsella
Bilophila

Revision editor(s): WikiWorks

Experiment 10


Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/30

Curated date: 2021/01/10

Curator: Lucy Mellor

Revision editor(s): WikiWorks

Source: Figure 3j

Description: Significantly diverse bacterial communities between healthy and eczema groups in age group 2-3 years

Abundance in Group 1: decreased abundance in child with eczema

NCBI Quality ControlLinks
Lachnospiraceae
Actinomycetota
Actinomycetes
Bifidobacteriales
Bifidobacteriaceae
Bifidobacterium
Pseudomonadota

Revision editor(s): WikiWorks