Patients With Reflux Esophagitis Possess a Possible Different Oral Microbiota Compared With Healthy Controls

Study information

Reviewed Marked as Reviewed by Atrayees on 2023-8-18

study design

prospective cohort

Citation

PMID PubMed identifier for scientific articles.

32733243

DOI Digital object identifier for electronic documents.

10.3389/fphar.2020.01000

URI

Authors

Wang B, Zhang Y, Zhao Q, Yan Y, Yang T, Xia Y, Chen H

Journal

Frontiers in pharmacology

Year

2020

Keywords:

biomarker, dysbiosis, high-throughput sequencing, oral microbiota, reflux esophagitis

Background and Aim: Reflux Esophagitis (RE) is caused by a variety of factors including anatomical and functional alterations involved in the pathogenesis. Oral microbiota is influenced by many factors such as heredity, nutrition, environments and host conditions, but little is known about relationship between oral microbiota and RE. The aim of this study was to explore whether the oral microbiota is changed in patients with RE. Methods: To clarify this correlation, fresh saliva samples from all subjects were collected and then oral microorganism diversity was analysed in 55 patients with RE and 51 controls via hypervariable tag sequencing and analyzing the V3-V4 region of the 16S rDNA gene. Results: There was no difference found in oral microbial diversity between RE patients and healthy controls by Shannon diversity index (p=0.60) and Simpson diversity index (p= 0.38). The abundance of Proteobacteria was lower, but Bacteroidetes was higher in patients with RE at the phylum level. At the genus level the abundances of Prevotella, Veillonella, Megasphaera, Peptostreptococcus, Atopobium, Oribacterium, Eubacterium, and Lachnoanaerobaculum were increased, while Neisseria, Streptococcus, Rothia, Granulicatella, Gemella, Aggregatibacter, Treponema, Campylobacter, Filifactor, Corynebacterium, and Lactivibrio were decreased in RE patients than the controls. Conclusions: Our study suggested oral microbial dysbiosis in patients with RE, and identified bacterial species with potential biomarker significance. Further studies are required to understand role of oral microbial dysbiosis in the pathogenesis of RE.

Experiment 1

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Reviewed Marked as Reviewed by Atrayees on 2023-8-18

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Atrayees

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Peptic esophagitis Esophagitis (& [reflux]) or esophageal reflux,Esophagitis, Peptic,Peptic esophagitis,peptic esophagitis,peptic reflux disease,Peptic reflux disease (disorder),Reflux esophagitis,reflux esophagitis,Reflux oesophagitis,reflux oesophagitis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: reflux esophagus patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: symptoms such as heartburn and regurgitation were in accordance with the endoscopic criteria of Los Angeles Grade and taken no drugs as treatment

Group 0 sample size Number of subjects in the control (unexposed) group: 51

Group 1 sample size Number of subjects in the case (exposed) group: 55

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 6 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Curated date: 2021/01/10

Curator: Rimsha Azhar

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Source: Figure 3

Description: Bacterial taxa diversities between Reflux Esophigitis (RE) and healthy controls

Abundance in Group 1: increased abundance in reflux esophagus patients

NCBI	Quality Control	Links
Atopobium
Bacteroidales
Bacteroidia
Bacteroidota
Coriobacteriaceae
Coriobacteriales
Eubacteriaceae
Eubacterium
Lachnoanaerobaculum
Lachnospiraceae
Megasphaera
Methylobacteriaceae
Negativicutes
Oribacterium
Peptostreptococcaceae
Peptostreptococcus
Prevotella
Prevotellaceae
Selenomonadales
Solobacterium
Veillonella
Veillonellaceae
Neomegalonema

Revision editor(s): Atrayees, WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Atrayees on 2023-8-18

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): Lwaldron, WikiWorks, Merit

Source: Figure 3

Description: Bacterial taxa diversities between Reflux Esophigitis (RE) and healthy controls

Abundance in Group 1: decreased abundance in reflux esophagus patients

NCBI	Quality Control	Links
Actinomycetales
Aggregatibacter
Bacillales
Bacillales Family X. Incertae Sedis
Bacilli
Betaproteobacteria
Burkholderiales
Campylobacter
Campylobacteraceae
Campylobacterales
Campylobacterota
Carnobacteriaceae
Corynebacteriaceae
Corynebacterium
Eikenella
Filifactor
Gardnerella
Gemella
Granulicella
Hallella
Johnsonella
Lactivibrio
Lactobacillales
Micrococcaceae
Neisseria
Neisseriaceae
Neisseriales
Pseudomonadota
Pseudoramibacter
Rothia
Spirochaetaceae
Spirochaetales
Spirochaetia
Spirochaetota
Streptococcaceae
Streptococcus
Synergistaceae
Synergistales
Synergistia
Synergistota
Syntrophomonadaceae
Treponema
Wolinella
Streptophyta
Cyanobacteriota

Revision editor(s): Lwaldron, WikiWorks, Merit