Distinct Features of Gut Microbiota in High-Altitude Tibetan and Middle-Altitude Han Hypertensive Patients

From BugSigDB
Reviewed Marked as Reviewed by Fatima on 2021/10/14
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Zhu LL, Ma ZJ, Ren M, Wei YM, Liao YH, Shen YL, Fan SM, Li L, Wu QX, Gao ZS, Song JF, Ma YL
Journal
Cardiology research and practice
Year
2020
Indigenous animals show unique gut microbiota (GM) in the Tibetan plateau. However, it is unknown whether the hypertensive indigenous people in plateau also have the distinct gut bacteria, different from those living in plains. We sequenced the V3-V4 region of the gut bacteria 16S ribosomal RNA (rRNA) gene of feces samples among hypertensive patients (HPs) and healthy individuals (HIs) from 3 distinct altitudes: Tibetans from high altitude (3600-4500 m, n = 38 and 34), Hans from middle altitude (2260 m, n = 49 and 35), and Hans from low altitude (13 m, n = 34 and 35) and then analyzed the GM composition among hypertensive and healthy subgroups using the bioinformatics analysis, respectively. The GM of high-altitude Tibetan and middle-altitude Han HPs presented greater α- and β-diversities, lower ratio of Firmicutes/Bacteroidetes (F/B), and higher abundance of beneficial Verrucomicrobia and Akkermansia than the low-altitudes HPs did. The GM of high-altitude Tibetan and middle-altitude HIs showed greater α-diversity and lower ratio of F/B than the low-altitudes HIs did. But, β-diversity and abundance of Verrucomicrobia and Akkermansia among different subgroups of HIs did not show any differences. Conclusively, the high-altitude Tibetan and middle-altitude Han HPs have a distinct feature of GM, which may be important in their adaptation to hypertension in the plateau environments.

Experiment 1


Reviewed Marked as Reviewed by Fatima on 2021/07/28

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Fatima, Rimsha, Manuela, WikiWorks

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Hypertension (hypertensive disease) or (hypertension),[X]Hypertensive diseases,[X]Hypertensive diseases (disorder),Blood Pressure, High,blood pressure, high,blood pressure, increased,Blood Pressures, High,High Blood Pressure,high blood pressure,High blood pressure (& [essential hypertension]),High Blood Pressures,HTN,HTN - hypertension,hyperpiesia,hypertension,hypertension NOS,HYPERTENSIVE DISEASE,hypertensive disease,Hypertensive disease (disorder),hypertensive disease NOS,Hypertensive disease NOS (disorder),hypertensive disorder,increased blood pressure,pressure, high blood,SURG COMP - HYPERTENSION,vascular hypertensive disorder,Hypertension
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Han Hypertensive at Low Altitude (LHH)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Tibetan Hypertensive at High Altitude (HTH)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Tibetan participants with hypertension living at high altitudes.
Group 0 sample size Number of subjects in the control (unexposed) group
34
Group 1 sample size Number of subjects in the case (exposed) group
38
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
Matched on Factors on which subjects have been matched on in a case-control study
age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2c

Description:

Abundance in Group 1: decreased abundance in Tibetan Hypertensive at High Altitude (HTH)

NCBI Quality ControlLinks
Bacillota

Revision editor(s): Manuela

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2c

Description:

Abundance in Group 1: increased abundance in Tibetan Hypertensive at High Altitude (HTH)

NCBI Quality ControlLinks
Bacteroidota
Verrucomicrobiota

Revision editor(s): Manuela

Experiment 2


Reviewed Marked as Reviewed by Fatima on 2021/07/16

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Rimsha, Manuela, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Han Hypertensive at Middle Altitude (MHH)
Group 0 sample size Number of subjects in the control (unexposed) group
49

Lab analysis

Statistical Analysis

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2g

Description:

Abundance in Group 1: decreased abundance in Tibetan Hypertensive at High Altitude (HTH)

NCBI Quality ControlLinks
Candidatus Saccharibacteria
Pseudomonadota

Revision editor(s): Manuela

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2g

Description:

Abundance in Group 1: increased abundance in Tibetan Hypertensive at High Altitude (HTH)

NCBI Quality ControlLinks
Bacteroidota

Revision editor(s): Manuela

Experiment 3


Reviewed Marked as Reviewed by Fatima on 2021/07/16

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Rimsha, Manuela, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Han Healthy at Low Altitude (LHN)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Tibetan Healthy at High Altitude (HTN)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Tibetan healthy participants living at high altitudes.
Group 0 sample size Number of subjects in the control (unexposed) group
35
Group 1 sample size Number of subjects in the case (exposed) group
34

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2d

Description:

Abundance in Group 1: decreased abundance in Tibetan Healthy at High Altitude (HTN)

NCBI Quality ControlLinks
Candidatus Saccharibacteria
Fusobacteriota
Pseudomonadota

Revision editor(s): Manuela

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2d

Description:

Abundance in Group 1: increased abundance in Tibetan Healthy at High Altitude (HTN)

NCBI Quality ControlLinks
Bacteroidota

Revision editor(s): Manuela

Experiment 4


Reviewed Marked as Reviewed by Fatima on 2021/07/16

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Rimsha, Manuela, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Han Healthy at Middle Altitude (MHN)

Lab analysis

Statistical Analysis

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2h

Description:

Abundance in Group 1: decreased abundance in Tibetan Healthy at High Altitude (HTN)

NCBI Quality ControlLinks
Candidatus Saccharibacteria

Revision editor(s): Manuela

Experiment 5


Reviewed Marked as Reviewed by Fatima on 2021/10/14

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Rimsha, Manuela, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Han Hypertensive at Low Altitude (LHH)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Han Hypertensive at Middle Altitude (MHH)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Han hypertensive participants living at middle altitude.
Group 0 sample size Number of subjects in the control (unexposed) group
34
Group 1 sample size Number of subjects in the case (exposed) group
49

Lab analysis

Statistical Analysis

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2e

Description:

Abundance in Group 1: decreased abundance in Han Hypertensive at Middle Altitude (MHH)

NCBI Quality ControlLinks
Bacillota

Revision editor(s): Manuela

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2e

Description:

Abundance in Group 1: increased abundance in Han Hypertensive at Middle Altitude (MHH)

NCBI Quality ControlLinks
Acidobacteriota
Bacteroidota
Candidatus Saccharibacteria
Mycoplasmatota
Verrucomicrobiota

Revision editor(s): Manuela

Experiment 6


Reviewed Marked as Reviewed by Fatima on 2021/10/14

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Rimsha, Manuela, WikiWorks

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Han Healthy at Low Altitude (LHN)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Han Healthy at Middle Altitude (MHN)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Han healthy participants living at middle altitude.
Group 0 sample size Number of subjects in the control (unexposed) group
35
Group 1 sample size Number of subjects in the case (exposed) group
35

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2f

Description:

Abundance in Group 1: increased abundance in Han Healthy at Middle Altitude (MHN)

NCBI Quality ControlLinks
Bacteroidota

Revision editor(s): Manuela

Signature 2

Reviewed Marked as Reviewed by Lwaldron on 2021/02/18

Curated date: 2021/02/17

Curator: Manuela

Revision editor(s): Manuela

Source: Figure 2f

Description:

Abundance in Group 1: decreased abundance in Han Healthy at Middle Altitude (MHN)

NCBI Quality ControlLinks
Bacillota
Fusobacteriota

Revision editor(s): Manuela