Sequencing of 16S rRNA reveals a distinct salivary microbiome signature in Behçet's disease/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Curated date: 2021/01/10
Curator: WikiWorks
Revision editor(s): LGeistlinger, WikiWorks, ChiomaBlessing
Subjects
- Location of subjects
- Turkey
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Behcet's syndrome Adamantiades-Behcet disease,BD,Bechet syndrome,BEHCET DIS,Behcet Disease,Behcet disease,Behcet syndrome,Behcet's disease,Behcet's syndrome,Behcets Syndrome,Behet's syndrome,Behet's syndrome (disorder),Behçet disease,Behçet syndrome,Behçet's disease,Behçet's syndrome,Behçet-Adamantiades syndrome,Behçet’s disease,Morbus Behçet's syndrome,silk road disease,triple symptom complex,Triple-Symptom Complex,behcet's syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- behcet's disease
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with Behcet's disease
- Group 0 sample size Number of subjects in the control (unexposed) group
- 15
- Group 1 sample size Number of subjects in the case (exposed) group
- 31
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- decreased
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Differential abundant taxa in patients with Behcet's disease VS controls
Abundance in Group 1: increased abundance in behcet's disease
| NCBI | Quality Control | Links |
|---|---|---|
| Alloprevotella | ||
| Haemophilus parainfluenzae |
Revision editor(s): WikiWorks, ChiomaBlessing
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Differential abundant taxa in patients with Behcet's disease VS controls
Abundance in Group 1: decreased abundance in behcet's disease
Revision editor(s): WikiWorks, ChiomaBlessing
