Cervicovaginal microbiota dysbiosis correlates with HPV persistent infection

Study information

Needs review

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

33207260

DOI Digital object identifier for electronic documents.

10.1016/j.micpath.2020.104617

URI

Authors

Qingqing B, Jie Z, Songben Q, Juan C, Lei Z, Mu X

Journal

Microbial pathogenesis

Year

2021

Keywords:

16S rDNA sequencing, Cervicovaginal microbiota, HPV persistent Infection, Host immunosuppression

HPV persistent infection is a main event leading to the development of cervical intraepithelial neoplasia and cervical cancer. Earlier to distinguish HPV persistent and transient infection is meaningful but the methods are limited. This study used 16S rDNA sequencing to determine the cervicovaginal microbiota of HPV persistent infection, transient infection and health women. Sequences analysis was performed and according to subsequent statistical analysis, the structure of cervicovaginal microbiota of healthy and transient infection individuals is relatively single, Firmicutes occupy the main composition. However, that of the HPV persistent infection presented a complicated trend and the abundance of Proteobacteria, Actinobacteria, Bacteroidetes and Fusobacteria was higher. The significance p-values of the average species abundance of Firmicutes, Proteobacteria and Bacteroides between HPV persistent and transient infection groups were 0.003, 0.018 and 0.005, respectively. The study also found 36 biomarkers of cervicovaginal microbiota dysbiosis for LDA score>4 among different groups. At genus level, Prevotella, Sphingomonas and Anaerococcus correlated with HPV persistent infection. At species level, Lactobacillus iners correlated with HPV transient infection. Besides, local immune microenvironment was changed with cervicovaginal microbiota dysbiosis. Interleukin-6 and TNF-α were significantly upregulated in cervical secretions from HPV persistent infection compared with those from transient infection and healthy women. Peripheral blood Regulatory T cells and myeloid-derived suppressor cells in patients with HPV persistent infection were also significantly increased. In conclusion, this study identified cervicovaginal microbiota dysbiosis closely related to HPV persistent infection, which provided a new idea and method for the prevention of cervical cancer.

Experiment 1

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Curated date: 2021/03/20

Curator: Cynthia Anderson

Revision editor(s): Cynthia Anderson, WikiWorks, Peace Sandy

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Uterine cervix Canalis cervicis uteri,Caudal segment of uterus,Cervical canal,Cervical canal of uterus,Cervix,Cervix of uterus,Cervix uteri,Neck of uterus,Uterine cervix

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: human papilloma virus infection HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease

Group 0 name Corresponds to the control (unexposed) group for case-control studies: HPV-

Group 1 name Corresponds to the case (exposed) group for case-control studies: HPV+ persistence

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: HPV infection of the same type that lasts for more than 12 months

Group 0 sample size Number of subjects in the control (unexposed) group: 5

Group 1 sample size Number of subjects in the case (exposed) group: 6

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Ion Torrent

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Richness Number of species: increased

Signature 1

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Curated date: 2021/03/20

Curator: Cynthia Anderson

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Source: Figure 5

Description: LEfSe Analysis. A: LDA value distribution histogram

Abundance in Group 1: increased abundance in HPV+ persistence

NCBI	Quality Control	Links
Pseudomonadota
Alphaproteobacteria
Actinomycetota
Clostridia
Eubacteriales
Hyphomicrobiales
Bacteroidota
Bacteroidia
Gammaproteobacteria
Rhizobiaceae
Phyllobacterium
Veillonellaceae
Bacteroidales
Prevotellaceae
Sphingomonadales
Sphingomonadaceae
Sphingomonas
Anaerococcus
Sphingomonas leidyi
Prevotella
Porphyromonadaceae
Porphyromonas
Phocaeicola coprocola DSM 17136
Burkholderiaceae
Ruminiclostridium papyrosolvens
Dialister
Peptoniphilus

Revision editor(s): Cynthia Anderson

Signature 2

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Curated date: 2021/03/20

Curator: Cynthia Anderson

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Source: Figure 5

Description: LEfSe Analysis. A: LDA value distribution histogram

Abundance in Group 1: decreased abundance in HPV+ persistence

NCBI	Quality Control	Links
Lactobacillus
Lactobacillaceae
Lactobacillales
Bacilli
Bacillota

Revision editor(s): Cynthia Anderson

Experiment 2

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Curated date: 2021/03/20

Curator: Cynthia Anderson

Revision editor(s): Cynthia Anderson, WikiWorks

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: HPV+ transient

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: HPV infection cleared within 12 months

Group 1 sample size Number of subjects in the case (exposed) group: 4

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: Not specified