Cervicovaginal microbiota dysbiosis correlates with HPV persistent infection
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Qingqing B, Jie Z, Songben Q, Juan C, Lei Z, Mu X
Journal
Microbial pathogenesis
Year
2021
Keywords:
16S rDNA sequencing, Cervicovaginal microbiota, HPV persistent Infection, Host immunosuppression
HPV persistent infection is a main event leading to the development of cervical intraepithelial neoplasia and cervical cancer. Earlier to distinguish HPV persistent and transient infection is meaningful but the methods are limited. This study used 16S rDNA sequencing to determine the cervicovaginal microbiota of HPV persistent infection, transient infection and health women. Sequences analysis was performed and according to subsequent statistical analysis, the structure of cervicovaginal microbiota of healthy and transient infection individuals is relatively single, Firmicutes occupy the main composition. However, that of the HPV persistent infection presented a complicated trend and the abundance of Proteobacteria, Actinobacteria, Bacteroidetes and Fusobacteria was higher. The significance p-values of the average species abundance of Firmicutes, Proteobacteria and Bacteroides between HPV persistent and transient infection groups were 0.003, 0.018 and 0.005, respectively. The study also found 36 biomarkers of cervicovaginal microbiota dysbiosis for LDA score>4 among different groups. At genus level, Prevotella, Sphingomonas and Anaerococcus correlated with HPV persistent infection. At species level, Lactobacillus iners correlated with HPV transient infection. Besides, local immune microenvironment was changed with cervicovaginal microbiota dysbiosis. Interleukin-6 and TNF-α were significantly upregulated in cervical secretions from HPV persistent infection compared with those from transient infection and healthy women. Peripheral blood Regulatory T cells and myeloid-derived suppressor cells in patients with HPV persistent infection were also significantly increased. In conclusion, this study identified cervicovaginal microbiota dysbiosis closely related to HPV persistent infection, which provided a new idea and method for the prevention of cervical cancer.
Experiment 1
Reviewed Marked as Reviewed by Folakunmi on 2024-2-9
Curated date: 2021/03/20
Curator: Cynthia Anderson
Revision editor(s): Cynthia Anderson, WikiWorks, Peace Sandy, Folakunmi
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Uterine cervix Canalis cervicis uteri,Caudal segment of uterus,Cervical canal,Cervical canal of uterus,Cervix,Cervix of uterus,Cervix uteri,Neck of uterus,Uterine cervix,uterine cervix
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Human papilloma virus infection HPV,Human Papilloma Virus Infection,Human papilloma Virus infection,human papilloma virus infection,Human papillomavirus caused disease or disorder,Human papillomavirus disease or disorder,Human Papillomavirus Infection,Human Papillomavirus infection,Human papillomavirus infectious disease,Human papilloma virus infection
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- HPV- controls and HPV+ transient patients
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- HPV+ persistence
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- HPV infection of the same type that lasts for more than 12 months
- Group 0 sample size Number of subjects in the control (unexposed) group
- 9
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Ion Torrent
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-2-6
Source: Figure 5
Description: LEfSe Analysis. A: LDA value distribution histogram
Abundance in Group 1: increased abundance in HPV+ persistence
Revision editor(s): Cynthia Anderson
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-2-6
Source: Figure 5
Description: LEfSe Analysis. A: LDA value distribution histogram
Abundance in Group 1: decreased abundance in HPV+ persistence
| NCBI | Quality Control | Links |
|---|---|---|
| Bacilli | ||
| Bacillota | ||
| Lactobacillaceae | ||
| Lactobacillales | ||
| Lactobacillus | ||
| Lactobacillus iners |
Revision editor(s): Cynthia Anderson, Folakunmi
Experiment 2
Reviewed Marked as Reviewed by Folakunmi on 2024-2-6
Curated date: 2021/03/20
Curator: Cynthia Anderson
Revision editor(s): Cynthia Anderson, WikiWorks, Folakunmi
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- HPV- controls and HPV+ persistent patients
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- HPV+ transient
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- HPV infection cleared within 12 months
- Group 0 sample size Number of subjects in the control (unexposed) group
- 11
- Group 1 sample size Number of subjects in the case (exposed) group
- 4
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- decreased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-2-6
Source: Figure 5
Description: LEfSe Analysis. A: LDA value distribution histogram
Abundance in Group 1: increased abundance in HPV+ transient
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus iners |
Revision editor(s): Cynthia Anderson
Experiment 3
Reviewed Marked as Reviewed by Folakunmi on 2024-2-9
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- HPV+ transient
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- HPV+ persistent
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- patients whose HPV infection persisted for 12 months
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-2-9
Source: supplementary figure s1
Description: Lefse analysis between persistent HPV infection group and transient HPV infection group
Abundance in Group 1: increased abundance in HPV+ persistent
Revision editor(s): Folakunmi
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-2-9
Source: supplementary figure s1
Description: Lefse analysis between persistent HPV infection group and transient HPV infection group
Abundance in Group 1: decreased abundance in HPV+ persistent
| NCBI | Quality Control | Links |
|---|---|---|
| Bacilli | ||
| Bacillota | ||
| Lactobacillaceae | ||
| Lactobacillales | ||
| Lactobacillus | ||
| Lactobacillus iners | ||
| Bacteria |
Revision editor(s): Folakunmi
Experiment 4
Reviewed Marked as Reviewed by Folakunmi on 2024-2-9
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- HPV- controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- HPV+ persistence
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- HPV infection of the same type that lasts for more than 12 months
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-2-9
Source: supplementary figure s1
Description: Lefse analysis between persistent HPV infection group and transient HPV infection group
Abundance in Group 1: increased abundance in HPV+ persistence
Revision editor(s): Folakunmi
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-2-9
Source: supplementary figure s1
Description: Lefse analysis between persistent HPV infection group and transient HPV infection group
Abundance in Group 1: decreased abundance in HPV+ persistence
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillaceae | ||
| Lactobacillales | ||
| Lactobacillus | ||
| Bacillus | ||
| Bacillota | ||
| Lactobacillus iners |
Revision editor(s): Folakunmi
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