Changes of vaginal microbiota during cervical carcinogenesis in women with human papillomavirus infection

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/6

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

32941440

DOI Digital object identifier for electronic documents.

10.1371/journal.pone.0238705

URI

Authors

So KA, Yang EJ, Kim NR, Hong SR, Lee JH, Hwang CS, Shim SH, Lee SJ, Kim TJ

Journal

PloS one

Year

2020

OBJECTIVE: To evaluate the changes of vaginal microbiota during cervical carcinogenesis in women with high-risk human papillomavirus infection. MATERIALS AND METHODS: Vaginal microbiota was analyzed using next-generation sequencing in women with normal, cervical intraepithelial neoplasia (CIN), or cervical cancer. RESULTS: A marked decrease of Lactobacillus crispatus was found in the CIN/cancer groups compared with that in the normal group. The diversity of microorganisms increased in patients with CIN or cervical cancer with HPV infection. Atopobium vaginae (OR 4.33, 95% CI 1.15-16.32), Dialister invisus (OR 4.89, 95% CI 1.20-19.94), Finegoldia magna (OR 6.00, 95% CI 1.08-33.27), Gardnerella vaginalis (OR 7.43, 95% CI 1.78-31.04), Prevotella buccalis (OR 11.00, 95% CI 2.00-60.57), and Prevotella timonensis (OR 6.00, 95% CI 1.46-24.69) were significantly associated with the risk of CIN 2/3 or cervical cancer. CONCLUSION: Women with the CIN and cervical cancer showed a high diversity in vaginal microbiota. Depletion of Lactobacillus crispatus and increased abundance of anaerobic bacteria were detected in women with cervical disease.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/6

Curated date: 2021/03/21

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, LGeistlinger, Cynthia Anderson, Claregrieve1

Subjects

Location of subjects: South Korea

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Vaginal fluid Vaginal discharge,Vaginal secretion,Vaginal fluid,vaginal fluid

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Cervical glandular intraepithelial neoplasia Cervical glandular intraepithelial neoplasia,cervical glandular intraepithelial neoplasia

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: CIN2+/cervical cancer

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: patients with cervical intraepithelial neoplasia 2+ or cervical cancer confirmed by biopsy

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 20

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: Chi-Square

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/6

Curated date: 2021/03/21

Curator: Cynthia Anderson

Revision editor(s): Cynthia Anderson, Claregrieve1

Source: Table 2

Description: Differential microbial abundance between healthy controls and patients with cervical disease with CIN 2 or 3 and cervical cancer

Abundance in Group 1: increased abundance in CIN2+/cervical cancer

NCBI	Quality Control	Links
Dialister invisus
Fannyhessea vaginae
Finegoldia magna
Gardnerella vaginalis
Hoylesella buccalis
Hoylesella timonensis

Revision editor(s): Cynthia Anderson, Claregrieve1