Characterization and Analysis of the Skin Microbiota in Rosacea: A Case-Control Study
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Curated date: 2021/05/29
Curator: Madhubani Dey
Revision editor(s): Rimsha, Madhubani Dey, Claregrieve1, WikiWorks
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Nose , Skin of cheek Nasal sac,Nasus,Nose,Olfactory apparatus,Peripheral olfactory organ,Proboscis,nose,Cheek skin,Skin of cheek,skin of cheek
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Rosacea acne rosacea,Acne roscea,acne roscea,Acne, erythematosa,acne, erythematosa,rosacea,Rosacea
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Participants with papulopustular rosacea (PPR)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants with rosacea included individuals with diagnosed papulopustular rosacea (PPR): severity of rosacea assessed using the National Rosacea Society clinical grading system ranged from mild to moderate
- Group 0 sample size Number of subjects in the control (unexposed) group
- 19
- Group 1 sample size Number of subjects in the case (exposed) group
- 19
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 4 weeks for topical antibiotics, corticosteroids, and other anti-inflammatory medications, within 8 weeks for systemic antibiotics, corticosteroids, and other immunosuppressive agents
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- Metastats
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Matched on Factors on which subjects have been matched on in a case-control study
- age, race, sex
Alpha Diversity
- Richness Number of species
- unchanged
Signature 1
Source: Table 1
Description: Differential microbial abundance between papulopustular rosacea (PPR) patients and healthy controls
Abundance in Group 1: increased abundance in Participants with papulopustular rosacea (PPR)
NCBI | Quality Control | Links |
---|---|---|
Alloprevotella tannerae | ||
Campylobacter ureolyticus | ||
Corynebacterium kroppenstedtii | ||
Gleimia europaea | ||
Prevotella intermedia |
Revision editor(s): Madhubani Dey, Claregrieve1
Signature 2
Source: Table 1
Description: Differential microbial abundance between papulopustular rosacea (PPR) patients and healthy controls
Abundance in Group 1: decreased abundance in Participants with papulopustular rosacea (PPR)
NCBI | Quality Control | Links |
---|---|---|
Anoxybacillus kestanbolensis | ||
Azorhizobium doebereinerae | ||
Cutibacterium granulosum | ||
Dysgonomonas gadei | ||
Providencia stuartii | ||
Shewanella algae |
Revision editor(s): Madhubani Dey, Claregrieve1
Experiment 2
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Participants with erythematotelangiectatic rosacea
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants with rosacea included individuals with diagnosed erythematotelangiectatic rosacea (ETR): severity of rosacea assessed using the National Rosacea Society clinical grading system ranged from mild to moderate
Lab analysis
Statistical Analysis
Alpha Diversity
- Richness Number of species
- unchanged
Signature 1
Source: Table 1
Description: Differential microbial abundance in erythematotelangiectatic rosacea (ETR) patients compared to healthy controls
Abundance in Group 1: decreased abundance in Participants with erythematotelangiectatic rosacea
NCBI | Quality Control | Links |
---|---|---|
Azorhizobium doebereinerae | ||
Porphyromonas endodontalis | ||
Providencia stuartii | ||
Roseomonas mucosa | ||
Shewanella algae | ||
Mediterraneibacter gnavus |
Revision editor(s): Madhubani Dey, Claregrieve1