Characterization and Analysis of the Skin Microbiota in Rosacea: A Case-Control Study

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

31502207

DOI Digital object identifier for electronic documents.

10.1007/s40257-019-00471-5

URI

https://pubmed.ncbi.nlm.nih.gov/31502207/

Authors

Rainer BM, Thompson KG, Antonescu C, Florea L, Mongodin EF, Bui J, Fischer AH, Pasieka HB, Garza LA, Kang S, Chien AL

Journal

American journal of clinical dermatology

Year

2020

BACKGROUND: The efficacy of antibiotics in rosacea treatment suggests a role for microorganisms in its pathophysiology. Growing concern over the adverse effects of antibiotic use presents a need for targeted antimicrobial treatment in rosacea. OBJECTIVE: We performed a case-control study to investigate the skin microbiota in patients with rosacea compared to controls matched by age, sex, and race. METHODS: Nineteen participants with rosacea, erythematotelangiectatic, papulopustular, or both, were matched to 19 rosacea-free controls. DNA was extracted from skin swabs of the nose and bilateral cheeks of participants. Sequencing of the V3V4 region of the bacterial 16S ribosomal RNA gene was performed using Illumina MiSeq and analyzed using QIIME/MetaStats 2.0 software. RESULTS: Compared with controls, skin microbiota in erythematotelangiectatic rosacea was depleted in Roseomonas mucosa (p = 0.004). Papulopustular rosacea was enriched in Campylobacter ureolyticus (p = 0.001), Corynebacterium kroppenstedtii (p = 0.008), and the oral flora Prevotella intermedia (p = 0.001). The highest relative abundance of C. kroppenstedtii was observed in patients with both erythematotelangiectatic and papulopustular rosacea (19.2%), followed by papulopustular (5.06%) and erythematotelangiectatic (1.21%) rosacea. C. kroppenstedtii was also associated with more extensive disease, with the highest relative abundance in rosacea affecting both the cheeks and nose (2.82%), followed by rosacea sparing the nose (1.93%), and controls (0.19%). CONCLUSIONS: The skin microbiota in individuals with rosacea displays changes from that of healthy skin, suggesting that further studies examining a potential role for the skin microbiota in the pathophysiology of rosacea may be warranted.

Experiment 1

Edit History Delete

Flag for review

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/05/29

Curator: Madhubani Dey

Revision editor(s): Rimsha, Madhubani Dey, Claregrieve1, WikiWorks

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Nose , Skin of cheek Nasal sac,Nasus,Nose,Olfactory apparatus,Peripheral olfactory organ,Proboscis,Cheek skin,Skin of cheek

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: rosacea acne rosacea,Acne roscea,acne roscea,Acne, erythematosa,acne, erythematosa,rosacea

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls
Group 1 name Corresponds to the case (exposed) group for case-control studies: Participants with papulopustular rosacea (PPR)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Participants with rosacea included individuals with diagnosed papulopustular rosacea (PPR): severity of rosacea assessed using the National Rosacea Society clinical grading system ranged from mild to moderate
Group 0 sample size Number of subjects in the control (unexposed) group: 19
Group 1 sample size Number of subjects in the case (exposed) group: 19
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 4 weeks for topical antibiotics, corticosteroids, and other anti-inflammatory medications, within 8 weeks for systemic antibiotics, corticosteroids, and other immunosuppressive agents

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: Metastats

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes
Matched on Factors on which subjects have been matched on in a case-control study: age, race, sex

Alpha Diversity

Richness Number of species: unchanged

Signature 1

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/05/30

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, Claregrieve1

Source: Table 1

Description: Differential microbial abundance between papulopustular rosacea (PPR) patients and healthy controls

Abundance in Group 1: increased abundance in Participants with papulopustular rosacea (PPR)

NCBI	Quality Control	Links
Alloprevotella tannerae
Campylobacter ureolyticus
Corynebacterium kroppenstedtii
Gleimia europaea
Prevotella intermedia

Revision editor(s): Madhubani Dey, Claregrieve1

Signature 2

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/05/30

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, Claregrieve1

Source: Table 1

Description: Differential microbial abundance between papulopustular rosacea (PPR) patients and healthy controls

Abundance in Group 1: decreased abundance in Participants with papulopustular rosacea (PPR)

NCBI	Quality Control	Links
Anoxybacillus kestanbolensis
Azorhizobium doebereinerae
Cutibacterium granulosum
Dysgonomonas gadei
Providencia stuartii
Shewanella algae

Revision editor(s): Madhubani Dey, Claregrieve1

Experiment 2

Edit History Delete

Flag for review

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/06/03

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, WikiWorks

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: Participants with erythematotelangiectatic rosacea
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Participants with rosacea included individuals with diagnosed erythematotelangiectatic rosacea (ETR): severity of rosacea assessed using the National Rosacea Society clinical grading system ranged from mild to moderate