Characterization and Analysis of the Skin Microbiota in Rosacea: A Case-Control Study

From BugSigDB
Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Rainer BM, Thompson KG, Antonescu C, Florea L, Mongodin EF, Bui J, Fischer AH, Pasieka HB, Garza LA, Kang S, Chien AL
Journal
American journal of clinical dermatology
Year
2020
BACKGROUND: The efficacy of antibiotics in rosacea treatment suggests a role for microorganisms in its pathophysiology. Growing concern over the adverse effects of antibiotic use presents a need for targeted antimicrobial treatment in rosacea. OBJECTIVE: We performed a case-control study to investigate the skin microbiota in patients with rosacea compared to controls matched by age, sex, and race. METHODS: Nineteen participants with rosacea, erythematotelangiectatic, papulopustular, or both, were matched to 19 rosacea-free controls. DNA was extracted from skin swabs of the nose and bilateral cheeks of participants. Sequencing of the V3V4 region of the bacterial 16S ribosomal RNA gene was performed using Illumina MiSeq and analyzed using QIIME/MetaStats 2.0 software. RESULTS: Compared with controls, skin microbiota in erythematotelangiectatic rosacea was depleted in Roseomonas mucosa (p = 0.004). Papulopustular rosacea was enriched in Campylobacter ureolyticus (p = 0.001), Corynebacterium kroppenstedtii (p = 0.008), and the oral flora Prevotella intermedia (p = 0.001). The highest relative abundance of C. kroppenstedtii was observed in patients with both erythematotelangiectatic and papulopustular rosacea (19.2%), followed by papulopustular (5.06%) and erythematotelangiectatic (1.21%) rosacea. C. kroppenstedtii was also associated with more extensive disease, with the highest relative abundance in rosacea affecting both the cheeks and nose (2.82%), followed by rosacea sparing the nose (1.93%), and controls (0.19%). CONCLUSIONS: The skin microbiota in individuals with rosacea displays changes from that of healthy skin, suggesting that further studies examining a potential role for the skin microbiota in the pathophysiology of rosacea may be warranted.

Experiment 1


Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/05/29

Curator: Madhubani Dey

Revision editor(s): WikiWorks, Rimsha, Madhubani Dey, Claregrieve1

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Nose , Skin of cheek Nasal sac,Nasus,Nose,Olfactory apparatus,Peripheral olfactory organ,Proboscis,nose,Cheek skin,Skin of cheek,skin of cheek
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Rosacea acne rosacea,Acne roscea,acne roscea,Acne, erythematosa,acne, erythematosa,rosacea,Rosacea
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
Participants with papulopustular rosacea (PPR)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants with rosacea included individuals with diagnosed papulopustular rosacea (PPR): severity of rosacea assessed using the National Rosacea Society clinical grading system ranged from mild to moderate
Group 0 sample size Number of subjects in the control (unexposed) group
19
Group 1 sample size Number of subjects in the case (exposed) group
19
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
4 weeks for topical antibiotics, corticosteroids, and other anti-inflammatory medications, within 8 weeks for systemic antibiotics, corticosteroids, and other immunosuppressive agents

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
Metastats
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Matched on Factors on which subjects have been matched on in a case-control study
age, race, sex

Alpha Diversity

Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/05/30

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, Claregrieve1

Source: Table 1

Description: Differential microbial abundance between papulopustular rosacea (PPR) patients and healthy controls

Abundance in Group 1: increased abundance in Participants with papulopustular rosacea (PPR)

NCBI Quality ControlLinks
Alloprevotella tannerae
Campylobacter ureolyticus
Corynebacterium kroppenstedtii
Gleimia europaea
Prevotella intermedia

Revision editor(s): Madhubani Dey, Claregrieve1

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/05/30

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, Claregrieve1

Source: Table 1

Description: Differential microbial abundance between papulopustular rosacea (PPR) patients and healthy controls

Abundance in Group 1: decreased abundance in Participants with papulopustular rosacea (PPR)

NCBI Quality ControlLinks
Anoxybacillus kestanbolensis
Azorhizobium doebereinerae
Cutibacterium granulosum
Dysgonomonas gadei
Providencia stuartii
Shewanella algae

Revision editor(s): Madhubani Dey, Claregrieve1

Experiment 2


Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/06/03

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, WikiWorks

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
Participants with erythematotelangiectatic rosacea
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants with rosacea included individuals with diagnosed erythematotelangiectatic rosacea (ETR): severity of rosacea assessed using the National Rosacea Society clinical grading system ranged from mild to moderate

Lab analysis

Statistical Analysis

Alpha Diversity

Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/09/29

Curated date: 2021/06/03

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, Claregrieve1

Source: Table 1

Description: Differential microbial abundance in erythematotelangiectatic rosacea (ETR) patients compared to healthy controls

Abundance in Group 1: decreased abundance in Participants with erythematotelangiectatic rosacea

NCBI Quality ControlLinks
Azorhizobium doebereinerae
Porphyromonas endodontalis
Providencia stuartii
Roseomonas mucosa
Shewanella algae
Mediterraneibacter gnavus

Revision editor(s): Madhubani Dey, Claregrieve1