Differential intestinal and oral microbiota features associated with gestational diabetes and maternal inflammation
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Xu Y, Zhang M, Zhang J, Sun Z, Ran L, Ban Y, Wang B, Hou X, Zhai S, Ren L, Wang M, Hu J
Journal
American journal of physiology. Endocrinology and metabolism
Year
2020
Keywords:
16S rRNA, gestational diabetes mellitus, intestinal microbiota, oral microbiota
Maternal microbiota is involved in many metabolic diseases. However, its role in the pathophysiology of gestational diabetes mellitus (GDM) remains unclear. In this case-control study, we performed a 16S rRNA sequencing-based microbial survey to compare the intestinal and oral microflora at third trimester during pregnancy between 30 GDM and 31 normal controls. Sequentially, a correlation-based network analysis was further performed to explore the interactions among microbiota, maternal and infant blood sugar, and inflammatory markers. Our results show that, compared with controls, the GDM cases showed significant differences in β-diversity and increased Gammaproteobacteria and Hemophilus in intestinal microbiota. Furthermore, the GDM cases showed lower α-diversity, increased Selenomonas and Bifidobacterium, and decreased Fusobacteria and Leptotrichia in oral microbiota. The ROC curve showed the area under the curve to be equal to 0.70 and 0.66 when using oral Leptotrichia or gut Hemophilus, respectively, to predict GDM status. In addition, the components and topography of microbial cooccurrence and coexclusion network were quite distinct by GDM status. In summary, intestinal and oral microorganisms in pregnant women are closely related to the status of GDM in the third trimester of pregnancy. The changes of intestinal and oral microbial features may be noninvasive biomarkers for monitoring the health management of GDM pregnancy.
Experiment 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/28
Curated date: 2021/06/09
Curator: Madhubani Dey
Revision editor(s): Madhubani Dey, Claregrieve1, WikiWorks, Peace Sandy
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Gestational diabetes diabetes in pregnancy,GDM,gestational diabetes,gestational diabetes mellitus,maternal gestational diabetes mellitus,Gestational diabetes
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Individuals with gestational diabetes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Individuals with gestational diabetes at third trimester during pregnancy
- Group 0 sample size Number of subjects in the control (unexposed) group
- 31
- Group 1 sample size Number of subjects in the case (exposed) group
- 30
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 2 weeks
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/28
Curated date: 2021/06/27
Curator: Madhubani Dey
Revision editor(s): Madhubani Dey, Claregrieve1, Davvve
Source: Figure 1 D
Description: Differential microbial abundance between individuals diagnosed with gestational diabetes and healthy controls.
Abundance in Group 1: increased abundance in Individuals with gestational diabetes
| NCBI | Quality Control | Links |
|---|---|---|
| Gammaproteobacteria | ||
| Haemophilus | ||
| Pasteurellaceae | ||
| Pasteurellales |
Revision editor(s): Madhubani Dey, Claregrieve1, Davvve
Signature 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/28
Curated date: 2021/06/27
Curator: Madhubani Dey
Revision editor(s): Madhubani Dey, Claregrieve1, Davvve
Source: Figure 1 D
Description: Differential microbial abundance between individuals diagnosed with gestational diabetes and healthy controls.
Abundance in Group 1: decreased abundance in Individuals with gestational diabetes
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Phascolarctobacterium | ||
| Rikenellaceae |
Revision editor(s): Madhubani Dey, Claregrieve1, Davvve
Experiment 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/28
Curated date: 2021/06/09
Curator: Madhubani Dey
Revision editor(s): Madhubani Dey, Claregrieve1, WikiWorks
Differences from previous experiment shown
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- Not specified
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/28
Source: Figure 2D
Description: Differential microbial abundance between individuals diagnosed with gestational diabetes and healthy controls
Abundance in Group 1: increased abundance in Individuals with gestational diabetes
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacteriaceae | ||
| Bifidobacteriales | ||
| Bifidobacterium | ||
| Selenomonas |
Revision editor(s): Madhubani Dey, Claregrieve1
Signature 2
Reviewed Marked as Reviewed by Claregrieve1 on 2022/11/28
Source: Figure 2D
Description: Differential microbial abundance between individuals diagnosed with gestational diabetes and healthy controls
Abundance in Group 1: decreased abundance in Individuals with gestational diabetes
| NCBI | Quality Control | Links |
|---|---|---|
| Fusobacteriota | ||
| Fusobacteriaceae | ||
| Fusobacteriia | ||
| Lachnoanaerobaculum | ||
| Lactobacillaceae | ||
| Leptotrichia | ||
| Leptotrichiaceae | ||
| Leuconostoc |
Revision editor(s): Madhubani Dey, Claregrieve1
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