Alteration of salivary microbiome in periodontitis with or without type-2 diabetes mellitus and metformin treatment/Experiment 1
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Reviewed Marked as Reviewed by Claregrieve1 on 2022/06/22
Curated date: 2021/06/17
Curator: Madhubani Dey
Revision editor(s): WikiWorks, Madhubani Dey, Peace Sandy, Davvve
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Periodontitis chronic pericementitis,inflammation of periodontium,Pericementitides,Pericementitis,Periodontitides,periodontitis,Periodontitis (disorder),Periodontitis, NOS,periodontium inflammation,periodontosis,Periodontitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy controls group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Individuals diagnosed with Periodontitis group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Individuals with Peridontitis; Periodontal disease was assessed by determining the PD, CAL, BI, GI, and PLI, which were then measured at six sites (i.e., mesiobuccal, buccal, distobuccal, distolingual, lingual, and mesiolingual) according to the Community Periodontal Index by an experienced dentist. Periodontitis patients had a PD of ≥ 5 mm and CAL of ≥ 3 mm.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 27
- Group 1 sample size Number of subjects in the case (exposed) group
- 31
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- patients treated with antibiotics within 3 months or those that received periodontal treatment within 6 months.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
Signature 1
Reviewed Marked as Reviewed by Claregrieve1 on 2022/06/22
Source: Figure 3, text
Description: Increased abundance of bacterial species in individuals with Periodontitis as compared to healthy controls
Abundance in Group 1: increased abundance in Individuals diagnosed with Periodontitis group
| NCBI | Quality Control | Links |
|---|---|---|
| Porphyromonas endodontalis | ||
| Segatella copri | ||
| Treponema medium | ||
| Alloprevotella rava | ||
| unclassified Sutterella | ||
| Treponema amylovorum | ||
| Faecalibacterium prausnitzii |
Revision editor(s): Madhubani Dey, Claregrieve1
